Abstract
Determination of ruscogenin in five Ruscus taxa in Turkey was performed by ultra performance liquid chromatography (UPLC). Chromatographic separation and determination of ruscogenin in herb and rhizome samples were accomplished on an Acquity UPLCTM BEH C18 (50 mm × 2.1 mm, i.d., 1.7 μm) column system using an isocratic elution with a mobile phase consisting of acetonitrile and water (90:10) (v/v) at a constant flow rate of 0.3 mL min−1. Chromatographic calibration graph of ruscogenin in the concentration range of 22–55 μg mL−1 was obtained by using the linear relationship between the concentration and the peak area measured at a wavelength of 200 nm. The method was validated and successfully applied to the quantitative analysis of the real samples containing ruscogenin of Ruscus species. The ruscogenin amount in the hydrolyzed herb of Ruscus aculeatus was higher than in the hydrolyzed rhizomes of Ruscus aculeatus. In the rhizomes of R. colchicus, R. hypoglossum and R. hypophyllum, the ruscogenin amount was higher than in the herbs.
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Acknowledgments
We are grateful to the Central Research Laboratory directorship of the Faculty of Pharmacy, Ankara University for their support during our experiments and also thank Prof. Dr. Yoshihiro Mimaki (Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan) who kindly provided us with ruscogenin and Dr. Erdal Dinç who kindly carried out the method validation.
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Application of Separation Techniques in Turkey
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Güvenç, A., Şatır, E. & Coşkun, M. Determination of Ruscogenin in Turkish Ruscus L. Species by UPLC. Chroma 66 (Suppl 1), 141–145 (2007). https://doi.org/10.1365/s10337-007-0351-2
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DOI: https://doi.org/10.1365/s10337-007-0351-2