Phase-resolved optical frequency domain imaging

Phase-resolved Doppler optical coherence tomography has been used to image blood flow dynamics in various tissues using both timedomain and spectral-domain optical coherence tomography techniques. In this manuscript, we present phase-resolved Doppler imaging with a highspeed optical frequency domain imaging system. We demonstrate that by correcting for spurious timing-induced phase errors, excellent flow sensitivity can be achieved, limited only by the imaging signal-to-noise ratio. Conventional and Doppler images showing flow in an Intralipid phantom and in human skin are presented. Additionally, we demonstrate the ability of phase-resolved OFDI to measure high flow rates without the deleterious effects of fringe washout. ©2005 Optical Society of America OCIS codes: (110.4500) Optical coherence tomography; (140.3600) Lasers, tunable; (170.3340) Laser Doppler velocimetry; (170.3880) Medical and biological imaging; (170.4500) Optical coherence tomography References and Links 1. D. 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Introduction
Optical coherence tomography (OCT) provides cross-sectional images of biological samples with resolution on the scale of several to tens of microns [1]. Microstructural contrast in conventional OCT results from differences in the optical scattering properties of various tissues. Additional biological or functional information can be obtained through the application of Doppler techniques to measure spatially-localized motion in the sample [2,3]. These methods, termed color Doppler OCT or optical Doppler tomography, have been applied for imaging blood flow in the skin [3][4][5][6][7], retina [8,9], and esophagus [10], among others. Simultaneous imaging of tissue microstructure and blood flow can significantly enhance the diagnostic utility of OCT. Initial Doppler OCT measurements were performed with timedomain OCT (TD-OCT) systems. Recently, it has been demonstrated that the application of frequency-domain ranging techniques [11,12] to OCT results in significantly improved sensitivity [13][14][15] and imaging speed [16,17] compared with conventional TD-OCT. Frequency-domain ranging has been implemented in OCT in two configurations, spectraldomain OCT (SD-OCT) [16,18,19] and optical frequency domain imaging (OFDI) [17,[20][21][22]. In SD-OCT, a spectrometer is used to record spectral fringes that result from the interference of a reference beam with light reflected from a sample. In OFDI, a narrowband wavelength-swept source and a single detector are used to record the same interferogram. Doppler imaging has been demonstrated recently in SD-OCT systems [8,23] but not, to our knowledge, in high-speed OFDI systems. Since OFDI is less prone to motion-induced fringe washout effects [24] associated with endoscopy and can provide a significantly larger depth range, it may become a preferred method for certain biomedical applications. Continued development of wavelength-swept laser sources promises further improvements in imaging speed [25] and resolution [26]. These advantages are compelling in several OCT applications, including Barrett's esophagus screening [27] and coronary imaging [28]. Combining OFDI with functional contrast through flow imaging may lead to an improved diagnostic capability, especially in screening for early cancer. In this manuscript, we demonstrate a high-speed phase-resolved OFDI system suitable for high-sensitivity flow measurements. A method for removing timing-induced noise that would otherwise limit flow sensitivity is presented, and the resulting system is shown to have signal-to-noise ratio (SNR) limited phase sensitivity. The capabilities of the phase-resolved OFDI system are demonstrated by imaging an Intralipid flow phantom and human dermal vessels in vivo.

Principle of Phase Resolved OCT
Phase-resolved OCT systems measure both the amplitude and phase of the light reflected from the sample as a function of depth. The amplitudes are used to generate traditional OCT structural images. Although the phases are generally random for biological samples, additional functional information can be obtained by measuring changes in phase. For example, spatially-resolved flow imaging can be accomplished by comparing the phases between successive A-lines at the same depth. A translation of the sample by distance δ during the time interval between two A-lines will induce a change in the measured phase of the reflected light given by Δφ = 2n k δ, where n is the refractive index of the sample and <k> is the average wavenumber of the OCT source (k=2π/λ). Calculating this phase difference at each depth yields spatially-resolved measurements of both the magnitude and direction of the axial (parallel to the imaging beam) flow velocity. Assuming that the imaging beam intersects the flow velocity vector at an angle β, the flow velocity is given by where τ is the time between A-lines. This method was originally demonstrated in TD-OCT [7] and later in SD-OCT [8], and is the method applied in the current work. To ensure correlation between the phase measurements of successive A-lines, the transverse displacement of the imaging beam between A-lines must be small relative to the beam size. This constraint can be met by effectively over sampling in the transverse direction. The sensitivity of the flow measurement is fundamentally limited by the phase sensitivity of the OCT system, which, in turn, is limited by the OCT system noise floor. It has been shown that the noise in the measured phase difference of a given signal, σ Δφ 2 (rad 2 ), with an SNR Note that, because calculation of the phase difference requires two measurements of phase, the noise level of phase difference measurements as given by Eq. (1) is twice that of single phase measurements. Most signals reflected from biological samples have SNRs below 50 dB, suggesting an ultimate phase difference measurement accuracy of ~3 mrad (this corresponds to a flow velocity of 0.02 mm/s at β = 80˚, n=1.3, and τ --1 = 15.6 kHz). Signals returning from depths greater than several hundred microns, where blood vessels are likely to be located, typically have SNRs below 30 dB and would yield ultimate phase accuracies of ~30 mrad. To achieve high-sensitivity flow imaging, other (less fundamental) noise sources, including interferometric instabilities, should be minimized such that phase sensitivity is SNR-limited up to an SNR of approximately 50 dB.

Phase-Resolved OFDI
Because flow is calculated from the phase difference between successive A-lines, it is essential that phase measurements be repeatable from one A-line to the next. Changes in the measured phase resulting from systematic or interferometric instabilities increase the phase noise floor of the system, reducing the ability of the system to image low flow rates. In SD-OCT, the inherent stability of the source, interferometer, and spectrometer enables highly repeatable phase measurements and, correspondingly, high-sensitivity flow imaging. In OFDI, variations in the synchronization/timing of the wavelength-swept source relative to the acquisition electronics can induce variations in the measurement of phase that degrade sensitivity. This effect is examined in the following section. Figure 1 depicts the OFDI system used in this paper [17]. The system comprises three modules: the wavelength-swept source, the interferometer, and the digital acquisition (DAQ) electronics. The source is constructed from a fiber ring-laser using a semiconductor optical amplifier (SOA) as the gain element. An intracavity rapidly-tuned narrowband optical filter is constructed using a polygon mirror [31]. Each facet of the polygon mirror generates a single sweep of the laser output as it rotates through the beam path of the telescope. The source has an output wavelength range from 1278 nm to 1388 nm, a repetition rate of 15.6 kHz, and an instantaneous linewidth of ~0.13 nm. The polarized source output is coupled to the interferometer where it is split into a reference arm and a sample arm. The sample arm light is delivered to and collected from the sample through a single fiber. An optical circulator directs the reflected light to the interferometer output coupler. The reference arm light reflects from the reference mirror, passes through a polarizer to remove any acquired variation in polarization state as a function of wavelength, and is directed to the other port of the output coupler. Resulting interference fringes are detected on both output ports. These signals are subtracted in a balanced-receiver configuration that reduces source intensity noise as well as auto-correlation noise from the sample. A portion of the reference arm light is directed to a narrowband fiber Bragg grating (FBG). A reflected optical pulse is generated when the wavelength of the source matches the FBG reflection wavelength. This pulse is detected and converted into a TTL signal with a low-to-high transition that is coincident with the optical pulse and a high-to-low transition that is electrically tunable. The DAQ board (National Instruments NI-6115, 10 MS/s) digitizes the balanced-receiver output using the high-to-low transition of the FBG-generated TTL pulse as a trigger signal. In response to the trigger signal, the DAQ board performs N analog-to-digital (A2D) conversions at its internal sample clock rate where the conversion occurs on the sample clock transitions. Although the polygon filter is driven by a signal derived from the sample clock, the phasing of the swept source output and the sample clock drifts slowly over time, causing the trigger signal to fall arbitrarily within the sample clock cycle. As a result, the delay between the trigger signal and the subsequent A2D conversion can vary by one period of the sample clock. To understand how this variation in delay affects phase-resolved measurements, consider the interference fringe resulting from a stationary mirror at depth z with reflectivity R. The resulting interference signal, S(t), is given by

Impact of source/acquisition timing variations in phase-resolved OFDI
where the laser is assumed to sweep linearly in wavenumber, i.e., k(t) = k o +αt (α = -6.1 x 10 9 m -1 s -1 for our source). Because in practice the source sweep contains higher-order terms, the detected signal is resampled in time to recover a signal given by Eq. (2) [17]. The parameter ε describes the delay between the trigger signal and the subsequent A2D conversion. This delay ranges from 0 to the sample clock period T cl ( = 0.1 μs) and is different for each A-line. The phase of the interference fringe associated with the mirror is given by φ = 2k o z+2αzε, and the range of phase differences between successive A-lines, Δφ, is given by where Δε is the change in the delay, ε, from one A-line to the next and is maximally equal to T cl . A normalized depth parameter Ζ = 2zαT cl /π has been defined where Ζ = 0 at the pathmatched depth and Ζ = 1 at the Nyquist-limited imaging depth. Equation 3 indicates that timing variations produce phase jumps that increase linearly with depth up to maximum value of π at the Nyquist-limited imaging depth (Ζ = 1). As shown previously, SNR-limited noise levels can be as low as a several mrads and therefore phase differences of the magnitude predicted by Eq. (3), if uncorrected, would severely degrade the sensitivity of the system.

Correction of timing-induced errors
There are several potential methods to correct the timing-induced phase jumps. Optical generation of the sample clock would improve the synchronization between the source and the DAQ board and reduced timing-induced phase jumps. To achieve this, a small portion of the source output could be directed to a periodic optical filter, generating an optical clock signal that is converted to a suitable TTL sample clock signal [20]. When the laser sweeps nonlinearly in k, the resulting sample clock is frequency chirped, and abruptly changes frequency between the end of one sweep and the beginning of the next. As such, there are potential compatibility issues between optically generated clocks and higher-speed (~100 MS/s) DAQ boards which incorporate phase-locked loop circuits on the external sample clock input. A second solution is to subtract from the measured phase-difference of each A-line pair that portion which varies linearly with depth in a manner similar to a correction technique (C) 2005 OSA 11 July 2005 / Vol. 13, No. 14 / OPTICS EXPRESS 5487 applied in phase-resolved TD-OCT systems [6]. Although straightforward and likely valid for cases in which flow is localized in a small region, this approach can distort the measured flow by subtracting linear portions of actual flow distributions.
To allow for the accurate measurement of arbitrary flow distributions, we have implemented a solution in which a separate calibration signal is used to measure timinginduced phase variations. These variations are then subtracted from the measured phase differences at all remaining depths. Figure 2(a) shows a sample arm modified to provide this calibration signal. A 1% tap coupler is used to direct light to both a stationary calibration mirror (1% port) and the sample to be imaged (99% port). The calibration mirror is positioned such that its resultant signal appears near the maximum imaging depth, which is optimal for two reasons. Firstly, the calibration mirror creates a line artifact in the image. By locating the calibration mirror near the maximum imaging depth, this artifact appears near the image edge, minimizing the degree to which it can obscure the sample image. Secondly, the magnitude of the timing-induced phase differences is maximized at large depths and can therefore be most accurately measured at these depths. Hereafter, the signal from the calibration mirror is referred to as the calibration signal and the signal from the sample is referred to as the sample signal. The amplitude of the calibration signal is adjusted to ensure that it is large enough to dominates the sample signal at large depths but not so large that it induces significant autocorrelation noise [12]. Figure 2  Corrected phase differences are calculated by subtracting a fraction of the measured phase difference of the calibration signal from the measured phase difference of the sample signal. The magnitude of the applied correction is scaled linearly with the sample signal depth as dictated by Eq. (3). In the following, the parameters Δφ i , j and Δˆ φ i , j are used to describe the directly measured phase difference and calculated corrected phase difference, respectively, at depth index i between A-lines j and (j-1). If the calibration signal is located at depth index m, the corrected phase difference at depth index i is calculated as The first term is the measured phase difference at depth index i and the second term is the applied correction, scaled according to the sample signal depth by the multiplicative factor (i/m). Appropriate phase unwrapping is performed on all measurements. Finally, bulk motion artifacts can be removed by subtracting the median phase difference from each A-line pair [6].

Measured phase sensitivity
The phase sensitivity of the system is given by the noise level of the corrected phase differences. Ideally, the correction procedure described by Eq. (4) reduces the impact of timing-induced phase jumps to negligible levels, leaving only the fundamental SNR-limited noise. To test this, a stationary mirror was used to generate a sample signal and corrected phase differences were calculated according to Eq. (4). The measured phase (φ), phase difference (Δφ), and corrected phase difference ( Δˆ φ ) of a sample signal at depth Ζ = 0.54 are plotted in Fig. 3(a)-(c), respectively. A depth of Ζ =1 corresponds to 2.6 mm in these measurements. The imperfect synchronization between the source and sample clock can be seen in the slow drift of the measured phase ( Fig. 3(a)). The large jumps in phase occur when the acquisition delay switches by one clock cycle. The magnitude of these phase jumps (see scale bar on right of Fig. 3(b)) is in agreement with the prediction of Eq. (3) for a signal at this depth. Figure 3(c) shows that the large phase jumps have been eliminated in the corrected phase difference. Additionally, the baseline noise level has been reduced by 7 dB due to the correction of smaller phase jumps resulting from variations in acquisition delay that are less than one clock cycle. To confirm the effectiveness of the correction method, the noise on the corrected phase differences was measured and compared to the SNR-limited noise predicted by Eqs. (1) and (4) as where Χ s is the SNR of the sample signal located at depth Ζ s and Χ c is the SNR of the calibration signal located at depth Ζ c . Phase difference measurements were performed at sample signal depths of Ζ s = 0.07 (0.2 mm), 0.54 (1.4 mm), and 0.84 (2.2 mm). In all measurements, the calibration signal was located at depth Ζ c = 0.96 (2.50 mm) and had an SNR Χ c ~ 31 dB. The sample signal SNR, Χ s , at each depth was adjusted from 10 dB to 50 dB through the use of a variable neutral-density filter located in the sample arm. Figure 4 plots the measured noise (calculated from 500 measurements) as a function of the sample signal SNR for two of the measured depths. The measurements show excellent agreement with the predicted noise level given by Eq. (5). Measurements at the intermediate depth of Ζ s = 0.54 (not shown) also show excellent agreement with predictions. This agreement indicates that the proposed correction method is able to reduce timing-induced phase noise to negligible levels for this range of SNRs. As indicated by Eq. (5), both the noise in the sample signal (first term) and the noise in the calibration signal (second term) contribute to the noise in the corrected signal, with the calibration signal contribution scaling with the sample signal depth, Ζ s . At the large depth shown in Fig 4(b), the calibration signal noise dominates when the sample signal SNR exceeds ~35 dB. Assuming that, in practice, the SNR of sample signals from large depths is limited below 30 dB, and that from shallow depths is limited below 50 dB, one can see from Fig. 4 that the resulting noise over this range is sample signal SNR-limited. In the event of higher sample signal SNRs, the calibration signal SNR can be increased to reduce its noise contribution.

Images
As a test of the phase-resolved OFDI system and correction method described above, pulsatile flow of 0.25% Intralipid in an 800 μm tube surrounded by stationary Intralipid of the same concentration was imaged. The angle between the tube and imaging beam was set at 80˚. Figure 5 shows the resulting structural and flow images. The images were acquired at an Aline rate of 15.6 kHz and are 3 mm in width and 2.6 mm in depth (in air). Each frame contains 2000 A-lines (displacement of 1.5 μm per A-line) yielding a frame rate of 7.8 frames per second. A moving median filter of size (20 x 3) pixels (30 x 25 μm) was applied to the corrected phase (flow) image. The flow image clearly differentiates the region of flow inside the tube from the surrounding stationary Intralipid. The phase difference and corresponding flow velocity (assuming an index of refraction of n = 1.32) are given in the colorbar. As mentioned previously, OFDI does not suffer from fringe washout due to sample motion. As such, phase-resolved OFDI is well-suited to measure high flow rates that induce phase shifts greater than 2π. To demonstrate this, the imaging beam was positioned at the center of the tube shown in Fig. 5 and A-lines were recorded without scanning the beam while an increased pulsatile flow rate was induced. Figure 6(a) shows the resulting M-mode image. Phase is mapped to a color-scale and the image coordinates are depth index (vertical axis) and time (horizontal axis). The increased flow rate results in measured phase differences that exceed 2π and produce phase wrapping artifacts. Because OFDI does not suffer from fringe washout effects, no SNR penalty is incurred as a result of these large phase shifts. The apparent depth, however, is displaced from the actual depth due to the large Doppler shift [24], an artifact that does not directly impact the ability of OFDI to measure large flow rates. Figure 6(b) shows the phase image unwrapped to remove discontinuities of 2π, yielding the depth-resolved timevarying flow distribution in the tube. Note the change of color-scale between Fig. 6(a) and 6(b). Figure 6(c) shows an unwrapped flow profile acquired at the time T as marked in the phase images. The images shown in Figure 6 are constructed by averaging over 100 consecutive A-lines. Figure 7 shows cross-sectional structural and flow images obtained from a human nailbed in vivo. The image size is the same as in Fig. 5. Two blood vessels (circled), not observed in the structural image, can be clearly seen in Fig. 7(b).

Conclusion
We have presented what we believe to be the first implementation of high-speed phaseresolved OFDI. The origin of timing-induced phase errors was explained and a method for removing them was demonstrated. The phase sensitivity was shown to be SNR-limited at several depths up to an SNR of 50 dB. Structural and flow images of a phantom of Intralipid flowing through a tube and blood flow in human skin, each acquired at an A-line rate of 15.6 kHz, were presented. The ability of phase-resolved OFDI to measure high flow rates in which phase differences significantly exceed 2π without fringe-washout effects was demonstrated.
Flow imaging through phase-resolved OFDI has the potential to enhance the diagnostic capabilities and the clinical impact of future OFDI-based systems.