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  • Biomedical Optical Spectroscopy and Diagnostics / Therapeutic Laser Applications
  • OSA Trends in Optics and Photonics (Optica Publishing Group, 1998),
  • paper BMC5
  • https://doi.org/10.1364/BOSD.1998.BMC5

A Comparative Study of One-Photon and Two-Photon Induced Fluorescence in Artery Tissue

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Abstract

Two-photon induced fluorescence (TPF) offers several well known advantages over conventional, one-photon fluorescence. [1,2]. Advantages include a deeper penetration depth into tissue by the longer wavelength photons, increased background rejection due to the separation of the excitation and signal wavelengths, higher spatial resolution, and less photobleaching and tissue damage. Furthermore, TPF follows different selection rules than conventional fluorescence, thus producing excitation of electronic states that may not be seen with single photon excitation. These benefits make TPF a promising technique for medical applications in which spectroscopic evaluation of tissue is desired.

© 1998 Optical Society of America

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