Abstract
Two-photon induced fluorescence (TPF) offers several well known advantages over conventional, one-photon fluorescence. [1,2]. Advantages include a deeper penetration depth into tissue by the longer wavelength photons, increased background rejection due to the separation of the excitation and signal wavelengths, higher spatial resolution, and less photobleaching and tissue damage. Furthermore, TPF follows different selection rules than conventional fluorescence, thus producing excitation of electronic states that may not be seen with single photon excitation. These benefits make TPF a promising technique for medical applications in which spectroscopic evaluation of tissue is desired.
© 1998 Optical Society of America
PDF ArticleMore Like This
Wei Zheng, Dong Li, Yicong Wu, and Jianan Y. Qu
BTuF38 Biomedical Optics (BIOMED) 2008
Peter T. C. So, Barry R. Masters, Enrico Gratton, and Irene E. Kochevar
JMA3 Advances in Optical Imaging and Photon Migration (BIOMED) 1998
Barry R. Masters, Peter T. C. So, William Mantulin, and Enrico Gratton
JMA4 Advances in Optical Imaging and Photon Migration (BIOMED) 1998