Refractive index of dark-adapted bacteriorhodopsin and tris(hydroxymethyl)aminomethane buffer between 390 and 880 nm

Zsuzsanna Heiner; Károly Osvay

doi:10.1364/AO.48.004610

Applied Optics
Vol. 48,
Issue 23,
pp. 4610-4615
(2009)
•https://doi.org/10.1364/AO.48.004610

Refractive index of dark-adapted bacteriorhodopsin and tris(hydroxymethyl)aminomethane buffer between 390 and 880 nm

Zsuzsanna Heiner and Károly Osvay

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Zsuzsanna Heiner and Károly Osvay, "Refractive index of dark-adapted bacteriorhodopsin and tris(hydroxymethyl)aminomethane buffer between 390 and 880 nm," Appl. Opt. 48, 4610-4615 (2009)

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Abstract

The refractivity of wild-type bacteriorhodopsin ( ${bR}_{WT}$ ) suspended in tris(hydroxymethyl)aminomethane (TRIS) buffer has been measured in the spectral range of $390 - 840 nm$ by the method of angle of minimal deviation with the use of a hollow glass prism. The refractive indices of pure ${bR}_{WT}$ as well as of TRIS buffer have been determined from the concentration dependent refraction values. Sellmeier-type dispersion equations have been fitted for both the TRIS buffer and pure ${bR}_{WT}$ .

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TRIS ( $10 mM$ , pH 7.0)	$A_{1} (10^{5})$	$A_{2} (10^{9})$	$λ_{1} [nm]$	$λ_{2} [nm]$
One-term Sellmeier	7.363698414	–	101.0293566	–
Two-term Sellmeier	7.757363056	1.17425171286	98.6571625	1040.2402896

bR Suspension	$A_{0} (10^{11})$	$λ_{0} [nm]$	$A_{1} (10^{4})$	$A_{2} (10^{8})$	$λ_{1} [nm]$	$λ_{2} [nm] (10^{3})$
Schwers form	6.838	559.8099	2.40692062	8.1543917	58.928371	1445.266549
Linear approx.	6.838	559.8099	2.42382645	7.5111726	58.540103	1012.51599

TRIS ( $10 mM$ , pH 7.0)	$A_{1} (10^{5})$	$A_{2} (10^{9})$	$λ_{1} [nm]$	$λ_{2} [nm]$
One-term Sellmeier	7.363698414	–	101.0293566	–
Two-term Sellmeier	7.757363056	1.17425171286	98.6571625	1040.2402896

bR Suspension	$A_{0} (10^{11})$	$λ_{0} [nm]$	$A_{1} (10^{4})$	$A_{2} (10^{8})$	$λ_{1} [nm]$	$λ_{2} [nm] (10^{3})$
Schwers form	6.838	559.8099	2.40692062	8.1543917	58.928371	1445.266549
Linear approx.	6.838	559.8099	2.42382645	7.5111726	58.540103	1012.51599

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Equations (6)

Applied Optics