Diversity Analysis by Seed Traits and ISSR Marker of Xanthium sibiricum Patrin Germplasm in Shandong ProvinceChinese Full Text
ZHAO Wei-hua;NIE Yuan-dong;HAN Fen-xia;DUN Bao-qing;WANG Zhi;LI Gui-ying;National Key Facility of Crop Gene Resources and Genetic Improvement / Biomass Energy Research Center / Institute of Crop Sciences,Chinese Academy of Agricultural Sciences;Plant Biotechnology Research Center,School of Agriculture and Biology,Shanghai Jiao Tong University;
Abstract: Twenty-nine Xanthium sibiricum Patrin germplasm resources from different ecological regions in Shandong province were analyzed for the genetic diversity of seed traits and ISSR molecular markers in order to provide a basis for germpalsm research and genetic improvement. The results showed that the average kernel rate was 32. 20%,and coefficient of variation was 26. 66%,diversity index was 1. 94. The average oil content was 30. 03%,and coefficient of variation was 20. 85%,diversity index was 1. 94. The average hundred grain weight was 9. 95g and coefficient of variation was 33. 46%,diversity index is 1. 96. 134 polymorphic loci were detected through 21 ISSR primers amplification with 6. 38 loci per primer and the ratio of polymorphic loci was 98. 53%. Different geographic and ecological group of Xanthium showed different genetic diversity. The highest genetic diversity was found in Luzhong mountain area,located in the junction of Jiaodong peninsula and inland,where gene exchanges took place easily. The lowest gene diversity was found in Lakeside lowland area,where it was blocked by Thai-Yi Mountain and Nansi Lake,the exchange of gene was limited. 29 Xanthium sibiricum resources in Shandong province plus one each from Hebei province and Shanxi province,clustering analysis,the results showed that the Siberian cocklebur resources in Shandong province and neighboring provinces had certain genetic exchange,but had own characteristic,and evolved relatively independent genetic resources system.
- DOI：
10.13430/j.cnki.jpgr.2014.03.031
- Series：
- Subject：
- Classification Code：
S567.219
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