Biological characteristics of a new Coxsackievirus B3 strain isolated from fecal specimens from a child with encephalitisChinese Full Text
LI Hua;YANG Hui-juan;WANG Dong-bao;KE Hua-xin;WANG Xi;SHEN Dong;WANG Qing-ling;MA Shao-hui;Institute of Medical Biology,Peking Union Medical College,Chinese Academy of Medical Science,Yunnan Province Key Laboratory for Development of Vaccines against Major Infectious Diseases;
Abstract: Objectives To analyze the biological characteristics of a human coxsackievirus B3strain（KMA103-09）isolated from fecal specimens from a child with encephalitis and to investigate its role in causing encephalitis. MethodsThe Coxsackievirus B3strain KMA103-09was propagated in KMB17cells,RD cells,VERO cells,Hep-2cells,Hela cells,and MRC-5cells.Cell growth was observed,and the 50%cell culture infective dose of the KMA103-09strain was detected in the various cell lines at different times after infection.A given amount of KMA103-09was injected intracerebrally in suckling mice and adult mice.The pathogenicity of the virus was observed and levels of serum IL-2,IL-4,IL-6,IFN-r,TNF,IL-17A,and IL-10were detected using a Cytokine Kit. Results The CVB3strain KMA103-09adapted as it grew and it had a significant cytopathic effect on the six cell lines.Hela cells were most susceptible to the KMA103-09strain and had the highest infection titer,8.62lgCCID50/ml,42hours post-infection.MRC-5cells were the least susceptible and had lowest infection titer,5.50lgCCID50/ml,120hours post-infection.The virus infected the one-day-old suckling mice and caused death.Pathological changes were found in the brain,heart,and/or lung tissue.Suckling mice had serum IL-6,IL-10,and TNF levels of 4.71pg/ml,21.08pg/ml,and 36.79pg/ml.There were statistically significant（P＜0.05）differences in these levels in suckling mice.The other four cytokines were not expressed.However,no pathogenicity was noted in adult mice and pathology of the brain,heart,and/or lung tissue revealed no obvious abnormalities. Conclusion Hela cells are the optimal cell line for growth of the Coxsackievirus B3strain KMA103-09.The KMA103-09strain is pathogenic in suckling mice but not in adult mice.
- DOI：
10.13350/j.cjpb.140402
- Series：
- Subject：
- Classification Code：
R512.3
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