Journal of Pathogen Biology. 2014, (02) Core

2010 strain of echovirus 20 isolated from Kunming in 2010Chinese Full Text

PAN Yue;SHAO Cong-wen;JI Ma;ZHU Yan-ju;ZHANG Yun-kun;MA Zhong-fei;LIU Jian-sheng;MA Shao-hui;Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Yunnan Key Laboratory for Research ＆ Development of Vaccines against Severe Infectious Diseases;

Abstract: Objective To analyze the genetic characteristics of the VP1gene of Echovirus 20isolated from the fecal specimens of a child infected with hepatitis A virus in Kunming in 2010. Methods The KM/EV20/2010strain was isolated from fecal specimens using human KMB17diploid cells,and its serotype was identified via a neutralization test using a WHO panel of sera for enterovirus typing and sera with a single strain of enterovirus.Viral RNA was extracted and then the VP1gene was amplified using RT-PCR and sequenced.The sequence of the VP1gene was compared to that of other strains of Echovirus 20in GenBank using Mega4.0software. Results The isolate was identified as an ECHO 20 serotype of enterovirus and the isolate was designated KM/EV20/2010.Its complete VP1gene had 867bp.There were no missing and inserted nucleotides.The strain’s nucleotides had similarity of 79.5%-91.7%to those of the Echovirus 20strains isolated from Shandong Province,China and its amino acids had similarity of 98.9%-99.6%to those of the Echovirus 20strains isolated from Shandong Province.Phylogenetic analysis based on the complete VP1sequence revealed that the complete VP1sequence of KM/EV20/2010was clustered in a distinct group with strains 94CF1666and 01352, which were respectively isolated from France in 1998and Shandong,China in 2001. Conclusion KM/EV20/2010is a strain of Echovirus 20.This strain can be isolated and grown in human KMB17diploid cells.

Keywords：

ECHO virus 20; VP1gene; sequence analysis;

DOI：
10.13350/j.cjpb.140201
Series：
Subject：
Classification Code：
S852.65

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Analysis of the complete VP1 nucleotide sequence of the KM/EV20/2010 strain of echovirus 20 isolated from Kunming in 2010Chinese Full Text

PAN Yue;SHAO Cong-wen;JI Ma;ZHU Yan-ju;ZHANG Yun-kun;MA Zhong-fei;LIU Jian-sheng;MA Shao-hui;Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Yunnan Key Laboratory for Research ＆ Development of Vaccines against Severe Infectious Diseases;

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