Knowledge Network Node
Mycosystema. 2014, (02) Core

Rapid identification of protoplast‐regenerated monokaryotic isolates of Auricularia auricula‐judae based on an allele InDel markerChinese Full Text

FAN Xiu‐Zhi;ZHOU Yan;BIAN Yin‐Bing;Institute of Applied Mycology, Huazhong Agricultural University;

Abstract: An insertion‐deletion length polymorphism（InDel） marker was designed based on the two alleles of glyceraldehyde‐ 3‐ phosphate dehydrogenase gene isolated from Auricularia auricula‐judae strain Au916. The protoplast regenerated strains from Au916 were examined to identify the monokaryons based on the InDel marker. Fluorescence microscopic examination and paired test were also conducted to verify the results of InDel detection. The results showed that the dikaryons and two different types of monokaryons from parent strain were separated from each other by the PCR patterns due to presence or absence of the InDel. Different genotype monokaryons could mate with each other to form clamp connection for producing fruiting body. In conclusion, the method based on the allele’s InDel could quickly and accurately identify the monokaryon from protoplast regenerated strains of A. auricula‐judae. Based on large‐scale sequencing of the fungal genomes, identification and application of InDel markers in monokaryon identification will become more universal.
Keywords：

Auricularia auricula‐judae; allele; insertion‐deletion length polymorphism; protoplast regeneration; monokaryon; GAPDH; fluorescence microscopic examination;

  • DOI：

    10.13346/j.mycosystema.130045

  • Series：

  • Subject：

  • Classification Code：

    S646.6

  • Mobile Reading
    Read on your phone instantly
    Step 1

    Scan QR Codes

    "Mobile CNKI-CNKI Express" App

    Step 2

    Open“CNKI Express”

    and click the scan icon in the upper left corner of the homepage.

    Step 3

    Scan QR Codes

    Read this article on your phone.

  • HTML
  • CAJ Download
  • PDF Download

Download the mobile appuse the app to scan this coderead the article.

Tips: Please download CAJViewer to view CAJ format full text.

Download: 296 Page: 273-279 Pagecount: 7 Size: 1020K

Related Literature
  • Similar Article
  • Reader Recommendation
  • Associated Author