Preliminary Research on Resistance to Virus of Chenopodium amaranticolor NDR1Chinese Full Text
GONG Qian-yuan;ZHANG Chao;WU Hua-nian;LI Wei-min;ZHANG Yong-qiang;Biotechnology Research Institute,Chinese Academy of Agricultural Sciences;
Abstract: Ca NDR1 a and Ca NDR1 b,2 Arabidopsis NDR1 homologous genes were cloned from Chenopodium amaranticolor to confirm their subcellular location,gene expresion,and analyze their resiutance to virus infection,thus providing a technological basis for transgenic disease resistance breeding.According to the previous results of transcriptome sequencing,Ca NDR1 a and Ca NDR1 b were cloned by RT-PCR.Then their gene expression level after virus infection was analyzed through Real-time PCR.The plant transient expression vectors were constructed,and Ca NDR1 a and Ca NDR1 b were found locating on cell membrane.The transgenic tobacco were obtained.T1plant’s resistance to tobacco mosaic virus（TMV） and cucumber mosaic virus（CMV） by enzyme-linked immunosorbent assay（ELISA）.Bio-informatic analysis revealed that Ca NDR1 a and Ca NDR1 b were sharing a striking similarity with Arabidopsis NDR1.Ca NDR1 a and Ca NDR1 b were up-regulated remarkably after Chenopodium amaranticolor inoculated by TMV and CMV.Ca NDR1 a and Ca NDR1 b were located in the cell membrane,which was not affected by virus infection.ELISA result showed that the resistance of part transgenic lines to TMV and CMV was reinforced.This study indicated that Ca NDR1 a and Ca NDR1 b were functional orthologs of Arabidopsis NDR1 and played a role in plant endogenous immune response.
- DOI：
10.13304/j.nykjdb.2014.059
- Series：
- Subject：
- Classification Code：
Q943.2
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