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Progress in Modern Biomedicine. 2014, (18)

Effect of EGCG on the Apoptosis and CUGBP1 Protein Expression of Human Lung Adenocarcinoma A549 Cells induced by LPSChinese Full Text

WU Qi;ZHANG Jing;CAO Liu-xia;LIU Xiao-ping;CHEN Chen;BIAN Jing-jing;Department of Histology and Embryology,Qingdao University of Medical College;Heze Medical College;

Abstract: Objective: To study the effects of epigallocatechin-3-gallate（EGCG） on the apoptosis and CUGBP1 expression of human lung adenocarcinoma A549 cells stimulated by lipopolysaccharide（LPS）. Methods: The proliferation and apoptosis of human lung adenocarcinoma A549 cells stimulated by LPS and EGCG were detected by MTT assay and flow cytometry respectively. The CUGBP1 protein expression of A549 cells stimulated by LPS and EGCG were examined by immunocytochemistry（ICC）. Results: LPS markedly stimulated the proliferation of A549 cells and increased CUGBP1 protein expression in the nucleus and cytoplasm, compared with normal control（P＜0.01）; EGCG inhibited the proliferation and obviously induced the apoptosis of A549 cells treated with LPS; EGCG also markedly antagonized the CUGBP1 protein expression in nucleus of A549 cells incubated with LPS（P＜0.01）; Quantitative analysis for the CUGBP1 protein level showed that at 4h or 24h, EGCG significantly inhibited the CUGBP1 expression of A549 cells stimulated by LPS. The relative expression amount of CUGBP1 protein stimulated by EGCG and LPS at 24 h（1210.565 ±3.46） was significantly higher than that at 4h（67.344±3.68）（t=927.164, P＜0.001）. Conclusion: EGCG can reverse the promotional effects of LPS on the A549 cell proliferation and CUGBP1 expression and induce the apoptosis.
Keywords：

LPS; Lung adenocarcinoma cell A549; Proliferation; CUGBP1; Epigallocatechin-3-galate（EGCG）; Cell apoptosis;

  • DOI：

    10.13241/j.cnki.pmb.2014.18.009

  • Series：

  • Subject：

  • Classification Code：

    R734.2

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