Influence of Crocin on Growth and Expression of Survivin and Livin in C6 Glioma CellsChinese Full Text
DONG Qiu-feng;YAN Zhi-feng;YANG Xin;HUO Jun-li;CHEN Xiao-yan;LI Juan;ZHEN Hai-ning;FEI Zhou;Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University;
Abstract: Objective: To study the effect of crocin on growth and expression of inhibitors of apoptosis protein（IAPs） survivin and livin in rat C6 glioma cells. Methods: The C6 glioma cells were cultured in vitro, treated with different concentration of crocin, and observed on different time points. The growth activity of C6 cells was investigated using cell growth curve drawn according to methyl thiazolyl tetrazolium（MTT）-based colorimetric assay. The changes of C6 cells morphology were observed under phase contrast microscope and using Hoechst fluorescent staining. The protein expression level of survivin and livin was analyzed using Western blot. Results: The growth of C6 cells was significantly suppressed in vitro by corcin, at 48 hours after treatment with culture solution including 2, 4 and 8 mg/ml of crocin, the optical density（OD） values of C6 cells were 0.732±0.013, 0.421±0.010 and 0.289±0.017 respectively, as well as the growth inhibition rates of C6 cells were 26.8±0.01%, 58.0±0.02% and 71.1±0.02% respectively, there were significant differences comparing the cell growth inhibition rates of experimental groups with both 4 mg/ml and 8 mg/ml of crocin with that of negative control group（P＜0.05 for both）. The changes of typical apoptotic morphology were presented in C6 cells of experimental groups. In addition, the Western blot analysis showed decreased protein expression of survivin and livin in C6 cells of experimental groups. Conclusion: Corcin can inhibit obviously the growth of C6 cells in vitro, and its inhibition effect is associated with inducing C6 cells apoptosis and down-regulating expression of IAPs surivin and livin in C6 cells.
- DOI：
10.13241/j.cnki.pmb.2014.16.011
- Series：
- Subject：
- Classification Code：
R739.41
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