Journal of Clinical Emergency. 2014, (06)

Protective effects of lipoic acid in rats with myocardial injury induced by acute paraquat intoxicationChinese Full Text

XIANG Xiaowei;HAN Jiyuan;Department of ICU,Hubei Medical College Affiliated Dongfeng General Hospital;Department of Emergency,Union Hospital,Tongji Medical College,Huazhong University Sience and Technology;

Abstract: Objective:To observe what mechanism lipoic acid had on myocardial injury caused by paraquat intake.Method:126mal Sprage-Dawley rats were randomly divided into 4groups:normal control one（NS,6rats）,paraquat poisoning one（PQ,24rats）,paraquat+reduced glutathione treatment group one（GSH,24rats）,and paraquat+lipoic acid treatment group one（LA,72）.For group LA,we subdivided it into three sub-groups,30mg/kg（24rats）,60mg/kg（24rats）,100mg/kg（24rats）.0.9% normal saline,50mg/kg paraquat was given to NS and PQ groups by gavage respectively.50mg/kg paraquat was given to GSH group,200mg/kg GSH by intraperitoneal injection half an hour later,repeat injection GSH for another 5days.50mg/kg paraqua was given to LA groups,then 30mg/kg,60mg/kg,100mg/kg LA for three subgroups respectively half an hour later,and repeat injection LA for another 5days.Each group 6rats killed randomly,got myocardium samples and abdominal aorta blood at same spot at 1st,3rd,5th,7th day after the model was built successfully,then observe the histological changes through microscope with HE staining and determine the levels of NF-κB,NOS,NO,AST、LDH、CK、CKMB levels.Result:①Histological result show that myocardial cell were in edema,hemorrhage,blob-shaped focal necrosis,broadened organizing interval,and was widely swelled,degenerated in PQ group’s group was less heavily than PQ groups at the same time points.Group LA was the least heavily in these four groups.②In group LA,the levels of NF-κB,NOS and NO was more lower than PQ and GSH group at the same time points（P＜0.01）.For groups LA,the levels of NF-κB,NOS and NO was higher than NS group（P＜0.05）,and with the increasing of LA injection,the activity of these biomarkers were decreasing（P＜0.05）.③For the levels of AST,LDH,CK,CKMB,it was lower in group LA than PQ and GSH at different time points（P＜0.01）,but higher than NS groups（P＜0.05）.With the increasing of LA injection,the activity of these markers were decreasing（P＜0.05）.Conclusion:Lipoic acid can reduce myocardial tissue damage caused by acute paraquat poisoning,that may be directly regulate the levels of NF-κB、NOS and NO,and then affect the contents of free radicals and inflammation,then reduce myocardial edema,hemorrhage and necrosis,and improve myocardial cell metabolism.

Keywords：

lipoic acid; paraquat; myocardial injury; NF-κB; NOS; NO; myocardial enzymes;

DOI：
10.13201/j.issn.1009-5918.2014.06.010
Series：
Subject：
Classification Code：
R595.4

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Protective effects of lipoic acid in rats with myocardial injury induced by acute paraquat intoxicationChinese Full Text

XIANG Xiaowei;HAN Jiyuan;Department of ICU,Hubei Medical College Affiliated Dongfeng General Hospital;Department of Emergency,Union Hospital,Tongji Medical College,Huazhong University Sience and Technology;

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