Archive BacDiveID:10.13145/bacdive8479.20201210.5

Strain identifier

BacDive ID: 8479

Type strain:

Species: Mycobacterium murale

Strain Designation: 220-96, MA 112/96

Strain history: CIP <- 1999, CCUG <- M. Salkinoja-Salonen, Helsinki, Finland <- M. A. Andersson

NCBI tax ID(s): 182220 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 11686

BacDive-ID: 8479

DSM-Number: 44340

keywords: aerobe, Bacteria, 16S sequence, genome sequence, mesophilic

description: Mycobacterium murale 220-96 is an aerobe, mesophilic bacterium that was isolated from water-damaged indoor building material.

strain history: <- R. Vuorio; MA 112/96 <- M. Andersson

doi: 10.13145/bacdive8479.20201210.5

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/actinobacteria
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Mycobacteriales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium murale
full scientific name: Mycobacterium murale Vuorio et al. 1999
synonyms
- @ref: 20215
- synonym: Mycolicibacterium murale

@ref: 11686

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium murale

full scientific name: Mycobacterium murale Vuorio et al. 1999

strain designation: 220-96, MA 112/96

type strain: yes

Morphology

colony morphology

@ref	colony color	incubation period	medium used
20046	Golden yellow (1004)	10-14 days	5006
20046		10-14 days	MB7H10
20046		10-14 days	MB7H11
54907		2-4 days

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
11686	MYCOBACTERIUM MEDIUM (DSMZ Medium 219)	yes	https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium219.pdf
11686	PEPTONE MEAT EXTRACT GLYCEROL AGAR (DSMZ Medium 250)	yes	https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium250.pdf
20046	MB7H10	yes		Name: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
20046	MB7H11	yes		Name: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
20046	5006	yes		Medium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
36255	MEDIUM 55 - for Mycobacterium	yes

culture temp

@ref	growth	type	temperature	range
20046	positive	optimum	37	mesophilic
11686	positive	growth	37	mesophilic
36255	positive	growth	22	psychrophilic
54907	positive	growth	30	mesophilic

Physiology and metabolism

oxygen tolerance

@ref: 54907
oxygen tolerance: aerobe

fatty acid profile

fatty acids

@ref	fatty acid	percentage	ECL
54907	C14:0	4.3	14
54907	C16:0	19.3	16
54907	C18:0	0.6	18
54907	C16:1 ω7c	2.6	15.819
54907	C16:1 ω7c/C15:0 ISO 2OH	10.8	15.85
54907	C16:1 ω9c	1.3	15.774
54907	C17:1 ω7c	16.1	16.818
54907	C17:1 ω8c	0.4	16.792
54907	C18:1 ω9c	28.2	17.769
54907	C18:2 ω6,9c/C18:0 ANTE	0.7	17.724
54907	C20:0 alcohol (C20:0 2OH alc)	11.4	18.835
54907	TBSA 10Me18:0	4.3	18.392

type of FA analysis: whole cell analysis
method/protocol: CCUG

API coryne

@ref	NIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG
20046	+	-	-	-	+	+	-	-	-	+	+	+	-	-	+	+	+	+	-

API zym

@ref	Alkaline phosphatase	Esterase	Esterase Lipase	Lipase	Leucine arylamidase	Valine arylamidase	Cystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
20046	-	+	+	-	-	-	-	-	-	-	-	-	+	+	-	+	-	-	-

Isolation, sampling and environmental information

isolation

@ref	sample type	country	origin.country	continent
11686	water-damaged indoor building material	Finland	FIN	Europe
54907	Water-damaged indoor building material	Finland	FIN	Europe

isolation source categories

Cat1: #Engineered
Cat2: #Built environment
Cat3: #Indoor

Safety information

risk assessment

@ref	biosafety level	biosafety level comment
11686	1	Risk group (German classification)
20046	1	German classification

Sequence information

16S sequences

@ref	description	accession	length	database	NCBI tax ID
20218	Mycobacterium murale 16S ribosomal RNA gene, partial sequence	HM770863	1472	ena	182220
20218	Mycobacterium murale strain CIP 105980 16S ribosomal RNA gene, partial sequence	AF547950	540	ena	182220
20218	Mycobacterium murale gene for 16S ribosomal RNA, partial sequence	AB537171	1459	ena	182220

Genome sequences

@ref: 66792
description: Mycolicibacterium murale JCM 13392
accession: GCA_010722995
assembly level: contig
database: ncbi
NCBI tax ID: 182220

GC content

@ref: 11686
GC-content: 72.9

External links

@ref: 11686

culture collection no.: CCUG 39728, JCM 13392, DSM 44340, HAMBI 2320

straininfo link

@ref	passport
20218	http://www.straininfo.net/strains/153040
20218	http://www.straininfo.net/strains/169501

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
11686	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44340)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-44340
20046	Wink, J.		http://www.dsmz.de/microorganisms/wink_pdf/DSM44340.pdf	Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215	D.Gleim, M.Kracht, N.Weiss et. al.		http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.html	Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
36255	Curators of the CIP		https://brclims.pasteur.fr/brcWeb/souche/detail/1/18059
54907	Curators of the CCUG	Culture Collection University of Gothenburg (CCUG) (CCUG 39728)	https://www.ccug.se/strain?id=39728
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)

General

Name and taxonomic classification

LPSN

synonyms

Morphology

colony morphology

Culture and growth conditions

culture medium

culture temp

Physiology and metabolism

oxygen tolerance

fatty acid profile

fatty acids

API coryne

API zym

Isolation, sampling and environmental information

isolation

isolation source categories

Safety information

risk assessment

Sequence information

16S sequences

Genome sequences

GC content

External links

straininfo link

Reference