Strain identifier

BacDive ID: 6830

Type strain: Yes

Species: Leuconostoc gelidum

Strain Designation: SML9

Strain history: CIP <- 1989, NCFB <- 1988, Shaw: strain SMl9, 1a21

NCBI tax ID(s): 927691 (strain), 1244 (species)

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General

@ref: 2230

BacDive-ID: 6830

DSM-Number: 5578

keywords: Bacteria, 16S sequence, genome sequence, anaerobe, mesophilic

description: Leuconostoc gelidum SML9 is an anaerobe, mesophilic bacterium that was isolated from vakuum-packaged beef.

NCBI tax id

NCBI tax idMatching level
1244species
927691strain

strain history

<- NCFB <- B.G. Shaw, SML9
NRIC 1778 <-- NCFB 2775.

doi: 10.13145/bacdive6830.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Leuconostocaceae
  • genus: Leuconostoc
  • species: Leuconostoc gelidum
  • full scientific name: Leuconostoc gelidum Shaw and Harding 1989

@ref: 2230

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Leuconostocaceae

genus: Leuconostoc

species: Leuconostoc gelidum subsp. gelidum

full scientific name: Leuconostoc gelidum subsp. gelidum (Shaw and Harding 1989) Rahkila et al. 2014

strain designation: SML9

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2230COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://bacmedia.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
2230MRS MEDIUM (DSMZ Medium 11)yeshttps://bacmedia.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
36378MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)

culture temp

@refgrowthtypetemperaturerange
2230positivegrowth25mesophilic
36378positivegrowth25mesophilic
67770positivegrowth25mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 2230
  • oxygen tolerance: anaerobe

murein

  • @ref: 2230
  • murein short key: A11.05
  • type: A3alpha L-Lys-L-Ala2

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose+builds acid from17057
68371salicin-builds acid from17814
68371esculin+builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
2230----+++----+++-----------+-+--+++--+----+---------

Isolation, sampling and environmental information

isolation

@refsample type
2230vakuum-packaged beef
67770Vacuum-packed meats

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Bottled, canned, packed food
#Engineered#Food production#Meat

Safety information

risk assessment

  • @ref: 2230
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Leuconostoc gelidum gene for 16S rRNA, strain:NRIC 1778AB0229211445ena927691
20218Leuconostoc gelidum DNA, 16S-23S rRNA intergenic spacer region, strain: NRIC 1778AB290439380ena927691
2230Leuconostoc gelidum 16S ribosomal RNA gene, partial sequenceAF1754021505ena927691
67770Leuconostoc gelidum subsp. gelidum gene for 16S ribosomal RNA, partial sequence, strain: JCM 9697LC0962211489ena1607839

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Leuconostoc gelidum subsp. gelidum KCTC 3527 KCTC 3527GCA_000166715contigncbi927691
66792Leuconostoc gelidum KCTC 3527927691.3wgspatric927691
66792Leuconostoc gelidum gelidum KCTC 3527651324068draftimg927691

GC content

@refGC-contentmethod
223037.0
6777037thermal denaturation, midpoint method (Tm)

External links

@ref: 2230

culture collection no.: DSM 5578, ATCC 49366, NCFB 2775, NCIMB 12897, JCM 9697, CCM 4189, CCUG 30198, CCUG 32249, CCUG 32696, CECT 4026, CIP 103318, KCTC 3527, LMG 18297, LMG 9850, NRIC 1778, VTT E-98972

straininfo link

@refpassport
20218http://www.straininfo.net/strains/152419
20218http://www.straininfo.net/strains/14635
20218http://www.straininfo.net/strains/14639
20218http://www.straininfo.net/strains/210939

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11034505Leuconostoc kimchii sp. nov., a new species from kimchi.Kim J, Chun J, Han HUInt J Syst Evol Microbiol10.1099/00207713-50-5-19152000Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Korea, Leuconostoc/chemistry/*classification/*genetics/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vegetables/*microbiologyGenetics
Enzymology17229480Characterization of a Leuconostoc gelidum bacteriophage from pork.Greer GG, Dilts BD, Ackermann HWInt J Food Microbiol10.1016/j.ijfoodmicro.2006.09.0212007Adipose Tissue/microbiology, Aerobiosis, Anaerobiosis, Animals, Bacteriophages/*isolation & purification, Colony Count, Microbial, Consumer Product Safety, Food Contamination/*analysis, Food Microbiology, Food Packaging/*methods, Food Preservation/*methods, Humans, Leuconostoc/*isolation & purification, Meat/*microbiology, Swine, Time Factors, VacuumPhylogeny
Pathogenicity17846581Effect of Leuconostoc spp. on the formation of Streptococcus mutans biofilm.Kang MS, Kang IC, Kim SM, Lee HC, Oh JSJ Microbiol25702007Biofilms/drug effects/*growth & development, Chromatography, Thin Layer, Dextrans/chemistry/metabolism/pharmacology, Leuconostoc/*metabolism, Streptococcus mutans/drug effects/*growth & developmentMetabolism
Genetics21131494Genome Sequence of Leuconostoc gelidum KCTC 3527, Isolated from Kimchi.Kim DS, Choi SH, Kim DW, Kim RN, Nam SH, Kang A, Kim A, Park HSJ Bacteriol10.1128/JB.01371-102010DNA, Bacterial/*chemistry/*genetics, Fermentation, *Genome, Bacterial, Korea, Leuconostoc/*genetics, Molecular Sequence Data, Sequence Analysis, DNA, Vegetables/*microbiologyTranscriptome
Phylogeny21705441Leuconostoc miyukkimchii sp. nov., isolated from brown algae (Undaria pinnatifida) kimchi.Lee SH, Park MS, Jung JY, Jeon COInt J Syst Evol Microbiol10.1099/ijs.0.032367-02011Anaerobiosis, Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Food Microbiology, Hydrogen-Ion Concentration, Korea, Leuconostoc/*classification/genetics/*isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Temperature, Undaria/*microbiologyBiotechnology
Phylogeny24431060Reclassification of Leuconostoc gasicomitatum as Leuconostoc gelidum subsp. gasicomitatum comb. nov., description of Leuconostoc gelidum subsp. aenigmaticum subsp. nov., designation of Leuconostoc gelidum subsp. gelidum subsp. nov. and emended description of Leuconostoc gelidum.Rahkila R, De Bruyne K, Johansson P, Vandamme P, Bjorkroth JInt J Syst Evol Microbiol10.1099/ijs.0.058263-02014Animals, Chickens, DNA, Bacterial/genetics, Genes, Bacterial, Leuconostoc/*classification/genetics/isolation & purification, Meat/*microbiology, Molecular Sequence Data, Multilocus Sequence Typing, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Ribotyping, Sequence Analysis, DNA, Swine, TurkeysGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2230Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5578)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5578
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36378Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15103
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid