Strain identifier

BacDive ID: 6562

Type strain: Yes

Species: Limosilactobacillus pontis

Strain history: CIP <- 1994, P. Stolz, Inst. für Lebensmitteltechnol., Germany: strain LTH 2587 <- Hohenheim Univ., Germany

NCBI tax ID(s): 1423794 (strain), 35787 (species)

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General

@ref: 3288

BacDive-ID: 6562

DSM-Number: 8475

keywords: anaerobe, Bacteria, 16S sequence, genome sequence, mesophilic

description: Lactobacillus pontis DSM 8475 is an anaerobe, mesophilic bacterium that was isolated from rye sourdough.

strain history: <- P. Stolz <- G. Böcker, LTH 2587

doi: 10.13145/bacdive6562.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/firmicutes
  • domain: Bacteria
  • phylum: Firmicutes
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Limosilactobacillus
  • species: Limosilactobacillus pontis
  • full scientific name: Limosilactobacillus pontis (Vogel et al. 1994) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus pontis

@ref: 3288

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus pontis

full scientific name: Lactobacillus pontis Vogel et al. 1994

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3288LACTOBACILLUS MEDIUM III (DSMZ Medium 638)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium638.pdf
38168MEDIUM 42 - for Lactobacillus pontisyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp broth (55.000 g);Lactobacillus pontis solution - M0209 (18.000 ml)

culture temp

@refgrowthtypetemperaturerange
3288positivegrowth30mesophilic
38168positivegrowth30mesophilic
51530positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 51530
  • oxygen tolerance: anaerobe

murein

  • @ref: 3288
  • murein short key: A21.04
  • type: A4ß L-Orn-D-Asp

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
3288-----+----------------------+--+------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
3288rye sourdoughGermany, StuttgartGermanyDEUEurope
51530Sour doughStuttgartGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Food
#Host#Plants#Herbaceous plants (Grass,Crops)

Safety information

risk assessment

  • @ref: 3288
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus pontis gene for 16S rRNA, partial sequence, strain: JCM 11051AB289267731ena35787
20218Lactobacillus pontis 16S rRNA gene, type strain LMG 14187AJ4220321480ena35787
3288L.pontis (LTH 2587) 16S rRNA geneX763291570ena35787

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus pontis LP475GCA_009428965completencbi35787
66792Lactobacillus pontis DSM 8475GCA_001435345scaffoldncbi1423794
66792Lactobacillus pontis UMB0683GCA_002940945contigncbi35787
66792Lactobacillus pontis DSM 84751423794.3wgspatric1423794
66792Lactobacillus pontis strain LP47535787.22completepatric35787
66792Lactobacillus pontis strain UMB068335787.3wgspatric35787

GC content

  • @ref: 3288
  • GC-content: 53
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 3288

culture collection no.: DSM 8475, ATCC 51518, JCM 11051, LMG 14187, CCUG 33456, LTH 2587

straininfo link

@refpassport
20218http://www.straininfo.net/strains/211417
20218http://www.straininfo.net/strains/35952
20218http://www.straininfo.net/strains/160995
20218http://www.straininfo.net/strains/35951

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3288Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8475)https://www.dsmz.de/collection/catalogue/details/culture/DSM-8475
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38168Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16118
51530Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 33456)https://www.ccug.se/strain?id=33456
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)