Strain identifier

BacDive ID: 5360

Type strain: Yes

Species: Enterococcus ureasiticus

Strain Designation: CCRI-16986, EN-67

Strain history: <- A. Maheux, Centre de recherche en infectiologie, Québec, Canada; EN-67 <- P. Cantin, Ministère du Développement durable, de l'Environnement et des Parcs du Québec, Canada

NCBI tax ID(s): 903984 (species)

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General

@ref: 16798

BacDive-ID: 5360

DSM-Number: 23328

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic

description: Enterococcus ureasiticus CCRI-16986 is a microaerophile, mesophilic bacterium that was isolated from well water.

NCBI tax id

  • NCBI tax id: 903984
  • Matching level: species

strain history

  • @ref: 16798
  • history: <- A. Maheux, Centre de recherche en infectiologie, Québec, Canada; EN-67 <- P. Cantin, Ministère du Développement durable, de l'Environnement et des Parcs du Québec, Canada

doi: 10.13145/bacdive5360.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus ureasiticus
  • full scientific name: Enterococcus ureasiticus Sistek et al. 2012

@ref: 16798

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus ureasiticus

full scientific name: Enterococcus ureasiticus Sistek et al. 2012

strain designation: CCRI-16986, EN-67

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no97.335
69480100positive

colony morphology

  • @ref: 16798
  • type of hemolysis: alpha
  • hemolysis ability: 1
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16798TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
16798COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
16798positivegrowth37mesophilic
61897positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16798microaerophile
61897microaerophile

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.987

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838129016arginine-hydrolysis
6838116988D-ribose+builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose+builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838116443D-tagatose+builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
16798catalase-1.11.1.6
16798cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
16798-+----++/--+++/-+---+--+++---+-++++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
16798well waterSte-Marie de BeauceCanadaCANNorth America
61897Well waterQuebec,Ste-Maire-de-BeuceCanadaCANNorth America2005-05-19

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic
#Engineered#Built environment#Water reservoir (Aquarium/pool)

Safety information

risk assessment

  • @ref: 16798
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16798
  • description: Enterococcus ureasiticus strain CCRI-16986 16S ribosomal RNA gene, partial sequence
  • accession: GU457264
  • length: 1290
  • database: ena
  • NCBI tax ID: 903984

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus ureasiticus DSM 23328GCA_001730285contigncbi903984
66792Enterococcus ureasiticus strain DSM 23328903984.3wgspatric903984
66792Enterococcus ureasiticus DSM 233282916555789draftimg903984

GC content

  • @ref: 16798
  • GC-content: 41.7
  • method: fluorimetric

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes89no
motileno94.328no
flagellatedno96.28no
gram-positiveyes90.949no
anaerobicno92.923yes
halophileyes77.706no
spore-formingno83.131no
thermophileno99.878yes
glucose-utilyes87.344no
aerobicno94.429yes
glucose-fermentyes85.418no

External links

@ref: 16798

culture collection no.: DSM 23328, CCUG 59304, LMG 26304

straininfo link

  • @ref: 74850
  • straininfo: 376038

literature

  • topic: Phylogeny
  • Pubmed-ID: 21788227
  • title: Enterococcus ureasiticus sp. nov. and Enterococcus quebecensis sp. nov., isolated from water.
  • authors: Sistek V, Maheux AF, Boissinot M, Bernard KA, Cantin P, Cleenwerck I, De Vos P, Bergeron MG
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.029033-0
  • year: 2011
  • mesh: Bacterial Proteins/genetics, DNA, Bacterial/genetics, Enterococcus/*classification/genetics/*isolation & purification, Fresh Water/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
16798Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23328)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23328
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
61897Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 59304)https://www.ccug.se/strain?id=59304
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68381Automatically annotated from API rID32STR
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
74850Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID376038.1StrainInfo: A central database for resolving microbial strain identifiers