Strain identifier

BacDive ID: 4505

Type strain: No

Species: Escherichia coli

Strain Designation: K12 KLF48/KL159, KLF48/KL159

Strain history: <- Osaka University <- CGSC <- K. Brooks Low

NCBI tax ID(s): 562 (species)

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General

@ref: 1766

BacDive-ID: 4505

DSM-Number: 4858

keywords: mesophilic, Bacteria

description: Escherichia coli K12 KLF48/KL159 is a mesophilic Proteobacterium of the family Enterobacteriaceae.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history: <- Osaka University <- CGSC <- K. Brooks Low

doi: 10.13145/bacdive4505.20211221.6

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/proteobacteria
  • domain: Bacteria
  • phylum: Proteobacteria
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 1766

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919

strain designation: K12 KLF48/KL159, KLF48/KL159

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1766SUPPLEMENTED (ARGININE) M9 MEDIUM (DSMZ Medium 450)yeshttps://bacmedia.dsmz.de/medium/450Name: SUPPLEMENTED (ARGININE) M9 MEDIUM (DSMZ Medium 450) Composition: Na2HPO4 6.0 g/l KH2PO4 3.0 g/l Glucose 2.0 g/l NH4Cl 1.0 g/l NaCl 0.5 g/l Thiamine-HCl x 2 H2O 0.373301 g/l MgSO4 0.12037 g/l Proline 0.02 g/l Arginine 0.02 g/l CaCl2 0.011098 g/l Distilled water
1766LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)yeshttps://bacmedia.dsmz.de/medium/381Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water

culture temp

  • @ref: 1766
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole+

enzymes

@refvalueactivityec
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARA
1766+-+-----+--++-++---+

Safety information

risk assessment

  • @ref: 1766
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

External links

@ref: 1766

culture collection no.: DSM 4858, CGSC 4302

straininfo link

@refpassport
20218http://www.straininfo.net/strains/159003
20218http://www.straininfo.net/strains/159004
20218http://www.straininfo.net/strains/159006

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1766Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4858)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4858
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274