Strain identifier
BacDive ID: 2731
Type strain: 
Species: Acetivibrio thermocellus
Strain Designation: JW 20
Strain history: <- A. Nolte, Universität Göttingen, Institut für Mikrobiologie, Germany; JW 20 <- J. Wiegel
NCBI tax ID(s): 1515 (species)
General
@ref: 1603
BacDive-ID: 2731
DSM-Number: 4150
keywords: alcohol production, thermophilic, anaerobe, Bacteria, genome sequence
description: Acetivibrio thermocellus JW 20 is an anaerobe, thermophilic bacterium that produces alcohol and was isolated from cotton.
NCBI tax id
- NCBI tax id: 1515
- Matching level: species
strain history: <- A. Nolte, Universität Göttingen, Institut für Mikrobiologie, Germany; JW 20 <- J. Wiegel
doi: 10.13145/bacdive2731.20220920.7
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Oscillospiraceae
- genus: Acetivibrio
- species: Acetivibrio thermocellus
- full scientific name: Acetivibrio thermocellus (Viljoen et al. 1926) Tindall 2019
synonyms
@ref synonym 20215 Ruminiclostridium thermocellum 20215 Clostridium thermocellum 20215 Hungateiclostridium thermocellum
@ref: 1603
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Oscillospiraceae
genus: Acetivibrio
species: Acetivibrio thermocellus
full scientific name: Acetivibrio thermocellus (Viljoen et al. 1926) Tindall 2019
strain designation: JW 20
type strain: no
Culture and growth conditions
culture medium
- @ref: 1603
- name: ACETIVIBRIO MEDIUM (DSMZ Medium 122)
- growth: yes
- link: https://bacmedia.dsmz.de/medium/122
- composition: Name: ACETIVIBRIO MEDIUM (DSMZ Medium 122) Composition: Cellulose 10.0 g/l Na2-ß-glycerophosphate x 5 H2O 6.0 g/l K2HPO4 5.5 g/l Cellobiose 5.0 g/l Yeast extract 4.5 g/l MgCl2 x 6 H2O 2.6 g/l KH2PO4 1.43 g/l (NH4)2SO4 1.3 g/l L-Glutathione 0.25 g/l CaCl2 x 2 H2O 0.13 g/l FeSO4 x 7 H2O 0.0011 g/l Sodium resazurin 0.0005 g/l Distilled water
culture temp
- @ref: 1603
- growth: positive
- type: growth
- temperature: 60
- range: thermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 1603
- oxygen tolerance: anaerobe
compound production
- @ref: 1603
- compound: ethanol
Isolation, sampling and environmental information
isolation
- @ref: 1603
- sample type: cotton
- geographic location: Louisiana
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Plants | #Shrub (Scrub) |
| #Engineered | #Industrial | #Engineered product |
| #Host Body Product | #Plant | #Cotton (other fibres) |
Safety information
risk assessment
- @ref: 1603
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
- @ref: 66792
- description: Clostridium thermocellum JW20
- accession: 492476.4
- assembly level: wgs
- database: patric
- NCBI tax ID: 492476
GC content
- @ref: 1603
- GC-content: 40.0
External links
@ref: 1603
culture collection no.: DSM 4150, ATCC 31549
straininfo link
| @ref | passport |
|---|---|
| 20218 | http://www.straininfo.net/strains/158666 |
| 20218 | http://www.straininfo.net/strains/108293 |
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 9212442 | Clostridium thermocellum JW20 (ATCC 31549) is a coculture with Thermoanaerobacter ethanolicus. | Erbeznik M, Jones CR, Dawson KA, Strobel HJ | Appl Environ Microbiol | 10.1128/aem.63.7.2949-2951.1997 | 1997 | Bacteria, Anaerobic/*genetics/*isolation & purification, Clostridium/*genetics/*isolation & purification, Coculture Techniques, Gram-Positive Asporogenous Rods, Irregular/*genetics/*isolation & purification, Molecular Sequence Data, Oligonucleotide Probes/genetics, Polymerase Chain Reaction/*methods, RNA, Ribosomal, 16S/*genetics, Sensitivity and Specificity, Soil Microbiology | Enzymology |
| 16347527 | Characterization of Clostridium thermocellum JW20. | Freier D, Mothershed CP, Wiegel J | Appl Environ Microbiol | 10.1128/aem.54.1.204-211.1988 | 1988 |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1603 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4150) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-4150 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) |