Strain identifier

BacDive ID: 16298

Type strain: Yes

Species: Streptomyces ruber

Strain history: KCC A-0131 <-- Y. Okami <-- ATCC 17754 <-- HACC 143.

NCBI tax ID(s): 83378 (species)

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General

@ref: 9446

BacDive-ID: 16298

DSM-Number: 40304

keywords: spore-forming, Bacteria, 16S sequence, genome sequence, mesophilic

description: Streptomyces ruber DSM 40304 is a spore-forming, mesophilic bacterium that builds an aerial mycelium.

strain history: <- E. B. Shirling, ISP (Chainia rubra) <- M. J. Thirumlachar, HACC-143

doi: 10.13145/bacdive16298.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Streptomycetales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces ruber
  • full scientific name: Streptomyces ruber (Thirumalachar 1955) Goodfellow et al. 1986

  • synonyms

    • @ref: 20215
    • synonym: Chainia rubra

@ref: 9446

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces ruber

full scientific name: Streptomyces ruber (Thirumalachar 1955) Goodfellow et al. 1986

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19435Red brown10-14 daysISP 2
19435Red10-14 daysISP 3
19435Orange10-14 daysISP 4
19435Red brown10-14 daysISP 5
19435Orange10-14 daysISP 6
19435Red brown10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19435yesAerial MyceliumRed brownISP 2
19435yesAerial MyceliumRedISP 3
19435yesAerial MyceliumRedISP 4
19435yesAerial MyceliumRedISP 5
19435noISP 6
19435yesAerial MyceliumRedISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9446GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf
19435ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19435ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19435ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19435ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19435ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19435ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
9446positivegrowth28mesophilic
19435positiveoptimum28mesophilic

Physiology and metabolism

spore formation

  • @ref: 19435
  • spore description: Formation of spore chains: spira, spore surface: smooth
  • spore formation: yes

halophily

  • @ref: 19435
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activity
1943562968cellulose-
1943516634raffinose-
1943526546rhamnose+/-
1943528757fructose+/-
1943529864mannitol+
1943517268myo-inositol-
1943518222xylose+
1943517992sucrose+
1943522599arabinose-
1943517234glucose+

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19435-------+-++

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
94461Risk group (German classification)
194351Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces ruber gene for 16S rRNA, partial sequenceAB018203121ena83378
20218Streptomyces ruber gene for 16S rRNA, partial sequence, strain: NBRC 14600AB1846041417ena83378

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces ruber NRRL ISP-5378GCA_000725575scaffoldncbi83378
66792Streptomyces ruber NRRL B-1661GCA_000719015contigncbi83378
66792Streptomyces ruber strain NRRL B-166183378.3wgspatric83378
66792Streptomyces ruber strain NRRL ISP-537883378.4wgspatric83378

External links

@ref: 9446

culture collection no.: DSM 40304, ATCC 17754, CBS 228.65, IFO 14600, ISP 5304, JCM 3131, KCC A-0131, NBRC 14600, NCIB 10983

straininfo link

@refpassport
20218http://www.straininfo.net/strains/165240
20218http://www.straininfo.net/strains/99373
20218http://www.straininfo.net/strains/231092
20218http://www.straininfo.net/strains/99375
20218http://www.straininfo.net/strains/99376
20218http://www.straininfo.net/strains/165242
20218http://www.straininfo.net/strains/165243
20218http://www.straininfo.net/strains/333447
20218http://www.straininfo.net/strains/99378

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9446Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40304)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40304
19435Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40304.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)