Strain identifier
BacDive ID: 158793
Type strain:
Species: Companilactobacillus metriopterae
Strain Designation: Hime5-1
Strain history: S. Kawasaki; Dept. of Biosci., Tokyo Univ. of Agric., Japan; Hime5-1.
NCBI tax ID(s): 1909267 (species)
General
@ref: 67232
BacDive-ID: 158793
DSM-Number: 103730
keywords: Gram-positive, Bacteria, facultative anaerobe, 16S sequence, genome sequence, rod-shaped
description: Companilactobacillus metriopterae Hime5-1 is a facultative anaerobe, Gram-positive, rod-shaped bacterium that was isolated from gut of grasshopper Metrioptera engelhardti.
NCBI tax id
- NCBI tax id: 1909267
- Matching level: species
strain history
<- S. Kawasaki, Dept. Bio-Science, Tokyo Univ. Agriculture, Japan; Hime5-1 <- S. Kawasaki and M. Chiba |
S. Kawasaki; Dept. of Biosci., Tokyo Univ. of Agric., Japan; Hime5-1. |
doi: 10.13145/bacdive158793.20211221.6
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/firmicutes
- domain: Bacteria
- phylum: Firmicutes
- class: Bacilli
- order: Lactobacillales
- family: Lactobacillaceae
- genus: Companilactobacillus
- species: Companilactobacillus metriopterae
- full scientific name: Companilactobacillus metriopterae (Chiba et al. 2018) Zheng et al. 2020
synonyms
@ref synonym 20215 Lactobacillus metriopterae 20215 Lactobacillus terrae
@ref: 67232
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Lactobacillaceae
genus: Companilactobacillus
species: Companilactobacillus metriopterae
full scientific name: Companilactobacillus metriopterae (Chiba et al. 2018) Zheng et al. 2020
strain designation: Hime5-1
type strain: yes
Morphology
cell morphology
- @ref: 65552
- gram stain: positive
- cell length: 0.6-1.2 µm
- cell width: 0.2 µm
- cell shape: rod-shaped
- motility: no
colony morphology
- @ref: 65552
- colony size: 1-2 mm
- colony color: yellowish
- incubation period: 2 days
- medium used: MRS agar
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
65552 | MRS agar | yes | ||
67232 | MRS MEDIUM (DSMZ Medium 11) | yes | https://bacmedia.dsmz.de/medium/11 | Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
65552 | positive | growth | 15-37 | |
65552 | no | growth | 10 | psychrophilic |
65552 | no | growth | 45 | thermophilic |
67232 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 65552
- oxygen tolerance: facultative anaerobe
spore formation
- @ref: 65552
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
65552 | NaCl | positive | growth | 7.0 %(w/v) |
65552 | NaCl | no | growth | 7.5 %(w/v) |
observation
@ref | observation |
---|---|
65552 | singly, in pairs or short chains |
65552 | homofermentative |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
65552 | 16808 | 2-dehydro-D-gluconate | - | builds acid from |
65552 | 58143 | 5-dehydro-D-gluconate | - | builds acid from |
65552 | 27613 | amygdalin | +/- | builds acid from |
65552 | 18305 | arbutin | - | builds acid from |
65552 | 17057 | cellobiose | +/- | builds acid from |
65552 | 15963 | ribitol | - | builds acid from |
65552 | 17108 | D-arabinose | - | builds acid from |
65552 | 18333 | D-arabitol | - | builds acid from |
65552 | 15824 | D-fructose | + | builds acid from |
65552 | 28847 | D-fucose | - | builds acid from |
65552 | 12936 | D-galactose | +/- | builds acid from |
65552 | 17634 | D-glucose | + | builds acid from |
65552 | 62318 | D-lyxose | - | builds acid from |
65552 | 16899 | D-mannitol | - | builds acid from |
65552 | 16024 | D-mannose | + | builds acid from |
65552 | 16988 | D-ribose | + | builds acid from |
65552 | 17924 | D-sorbitol | - | builds acid from |
65552 | 16443 | D-tagatose | - | builds acid from |
65552 | 65327 | D-xylose | - | builds acid from |
65552 | 16813 | galactitol | - | builds acid from |
65552 | 17113 | erythritol | - | builds acid from |
65552 | 4853 | esculin | + | builds acid from |
65552 | 28066 | gentiobiose | +/- | builds acid from |
65552 | 24265 | gluconate | - | builds acid from |
65552 | 17234 | glucose | - | builds gas from |
65552 | 17234 | glucose | + | fermentation |
65552 | 17754 | glycerol | - | builds acid from |
65552 | 28087 | glycogen | - | builds acid from |
65552 | 17268 | myo-inositol | - | builds acid from |
65552 | 15443 | inulin | - | builds acid from |
65552 | 30849 | L-arabinose | - | builds acid from |
65552 | 18403 | L-arabitol | - | builds acid from |
65552 | 18287 | L-fucose | - | builds acid from |
65552 | 62345 | L-rhamnose | - | builds acid from |
65552 | 17266 | L-sorbose | - | builds acid from |
65552 | 65328 | L-xylose | - | builds acid from |
65552 | 17716 | lactose | - | builds acid from |
65552 | 17306 | maltose | - | builds acid from |
65552 | 6731 | melezitose | - | builds acid from |
65552 | 28053 | melibiose | - | builds acid from |
65552 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
65552 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
65552 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
65552 | 506227 | N-acetylglucosamine | + | builds acid from |
65552 | 17632 | nitrate | - | reduction |
65552 | 16634 | raffinose | - | builds acid from |
65552 | 17814 | salicin | +/- | builds acid from |
65552 | 28017 | starch | - | builds acid from |
65552 | 17992 | sucrose | - | builds acid from |
65552 | 27082 | trehalose | +/- | builds acid from |
65552 | 32528 | turanose | - | builds acid from |
65552 | 17151 | xylitol | - | builds acid from |
enzymes
@ref | value | activity | ec |
---|---|---|---|
65552 | catalase | - | 1.11.1.6 |
65552 | arginine dihydrolase | - | 3.5.3.6 |
65552 | alkaline phosphatase | + | 3.1.3.1 |
65552 | proline-arylamidase | + | 3.4.11.5 |
65552 | phenylalanine arylamidase | + | |
65552 | tyrosine arylamidase | + | |
65552 | alanine arylamidase | + | 3.4.11.2 |
65552 | glycin arylamidase | + |
fatty acid profile
fatty acids
@ref fatty acid percentage 65552 C16:0 17.8 65552 C18:0 3.8 65552 C19:0 iso 2.3 65552 C18:1ω9c 52.1 65552 C16:1ω6c / C16:1ω7c 1 65552 unknown 18.846 / C19:1ω6c / C19:0 cycl 11.9 65552 C18:1ω6c / C18:1ω7c 10.3 - type of FA analysis: whole cell analysis
- incubation medium: MRS
- incubation temperature: 37
- incubation time: 2
- incubation_oxygen: anaerobic
- library/peak naming table: TSBA6
- system: MIS MIDI
- cutoff value:
Isolation, sampling and environmental information
isolation
@ref | sample type | sampling date | geographic location | country | origin.country | continent | latitude | longitude | enrichment culture | host species |
---|---|---|---|---|---|---|---|---|---|---|
65552 | gut of grasshopper Metrioptera engelhardti | 2015-09 | Nonomi plateau, Nagano Prefecture | Japan | JPN | Asia | 37 | 138.567 | MRS agar | |
67770 | Grasshopper, Metrioptera engelhardti (Japanese common name: himegisu) | mountainous areas of the Nonomi plateau in Nagano Pref. | Japan | JPN | Asia | Metrioptera engelhardti |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Arthropoda | #Insecta |
#Host Body-Site | #Gastrointestinal tract |
Sequence information
16S sequences
- @ref: 65552
- description: 16S rRNA gene sequence
- accession: LC190736
- database: nuccore
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Companilactobacillus metriopterae JCM 31635 | GCA_004117915 | contig | ncbi | 1909267 |
66792 | Lactobacillus metriopterae strain JCM 31635 | 1909267.3 | wgs | patric | 1909267 |
66792 | Companilactobacillus metriopterae JCM 31635 | 2881902429 | draft | img | 1909267 |
67770 | Companilactobacillus metriopterae strain JCM 31635, whole genome shotgun sequencing project | RSTD00000000 | ncbi | 1909267 |
GC content
@ref | GC-content | method |
---|---|---|
65552 | 37.3 | high performance liquid chromatography (HPLC) |
67770 | 32.8 | genome sequence analysis |
67770 | 37-37.6 | high performance liquid chromatography (HPLC) |
External links
@ref: 67232
culture collection no.: JCM 31635, DSM 103730, Hime 5-1
Reference
@id | authors | title | doi/url | journal | pubmed | catalogue |
---|---|---|---|---|---|---|
20215 | D.Gleim, M.Kracht, N.Weiss et. al. | Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since | http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.html | |||
65552 | Makoto Chiba, Takanori Itabashi, Koujiro Hirai, Mitsuo Sakamoto, Moriya Ohkuma, Taichiro Ishige and Shinji Kawasaki | Lactobacillus metriopterae sp. nov., a novel lactic acid bacterium isolated from the gut of grasshopper Metrioptera engelhardti | 10.1099/ijsem.0.002694 | IJSEM 68: 1484-1489 2018 | 29543150 | |
67232 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-103730 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103730) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ |