Strain identifier
BacDive ID: 158630
Type strain:
Species: Bacillus manusensis
Strain Designation: Ma50-6
Strain history: <- Sun Qinglei, Institute of Oceanology, China
NCBI tax ID(s): 2078954 (species)
version 5
General
@ref: 65353
BacDive-ID: 158630
keywords: thermophilic, spore-forming, Bacteria, 16S sequence, gram-positive, rod-shaped
description: Bacillus manusensis Ma50-6 is a spore-forming, thermophilic, gram-positive bacterium that was isolated from hydrothermal sediments .
doi: 10.13145/bacdive158630.20201210.5
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/firmicutes
- domain: Bacteria
- phylum: Firmicutes
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Bacillus
- species: Bacillus manusensis
- full scientific name: Bacillus manusensis Sun et al. 2018
@ref: 65353
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Caryophanales
family: Bacillaceae
genus: Bacillus
species: Bacillus manusensis
strain designation: Ma50-6
type strain: yes
Morphology
cell morphology
- @ref: 65353
- gram stain: positive
- cell length: 2-5 µm
- cell width: 0.4-0.6 µm
- cell shape: rod-shaped
Culture and growth conditions
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
65353 | positive | growth | 45-70 | thermophilic |
65353 | positive | optimum | 50-55 | thermophilic |
culture pH
@ref | ability | type | pH |
---|---|---|---|
65353 | positive | growth | 7-10 |
65353 | positive | optimum | 7 |
Physiology and metabolism
spore formation
- @ref: 65353
- spore description: Ellipsoidal endospores are formed centrally and usually cause the sporangia to swell
- type of spore: endospore
- spore formation: yes
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
65353 | NaCl | positive | growth | 0-8 %(w/v) |
65353 | NaCl | positive | optimum | 3 %(w/v) |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
65353 | 27613 | amygdalin | - | builds acid from |
65353 | 18305 | arbutin | - | builds acid from |
65353 | 27689 | decanoate | - | assimilation |
65353 | casein | - | hydrolysis | |
65353 | 17057 | cellobiose | - | builds acid from |
65353 | 15963 | ribitol | - | builds acid from |
65353 | 17108 | D-arabinose | - | builds acid from |
65353 | 18333 | D-arabitol | - | builds acid from |
65353 | 15824 | D-fructose | - | builds acid from |
65353 | 28847 | D-fucose | - | builds acid from |
65353 | 12936 | D-galactose | - | builds acid from |
65353 | 17634 | D-glucose | - | builds acid from |
65353 | 17634 | D-glucose | + | assimilation |
65353 | 62318 | D-lyxose | - | builds acid from |
65353 | 17306 | maltose | + | assimilation |
65353 | 16899 | D-mannitol | - | builds acid from |
65353 | 16899 | D-mannitol | + | assimilation |
65353 | 16024 | D-mannose | - | assimilation |
65353 | 16024 | D-mannose | - | builds acid from |
65353 | 16988 | D-ribose | - | builds acid from |
65353 | 17924 | D-sorbitol | - | builds acid from |
65353 | 16443 | D-tagatose | - | builds acid from |
65353 | 65327 | D-xylose | - | builds acid from |
65353 | 16813 | galactitol | - | builds acid from |
65353 | 17113 | erythritol | - | builds acid from |
65353 | 4853 | esculin | - | builds acid from |
65353 | 4853 | esculin | + | hydrolysis |
65353 | esculin ferric citrate | + | builds acid from | |
65353 | 28260 | galactose | - | builds acid from |
65353 | 28066 | gentiobiose | - | builds acid from |
65353 | 17234 | glucose | + | builds acid from |
65353 | 17754 | glycerol | - | builds acid from |
65353 | 28087 | glycogen | - | builds acid from |
65353 | 28087 | glycogen | + | builds acid from |
65353 | 17268 | myo-inositol | - | builds acid from |
65353 | 15443 | inulin | - | builds acid from |
65353 | 30849 | L-arabinose | - | assimilation |
65353 | 30849 | L-arabinose | - | builds acid from |
65353 | 18403 | L-arabitol | - | builds acid from |
65353 | 18287 | L-fucose | - | builds acid from |
65353 | 62345 | L-rhamnose | - | builds acid from |
65353 | 17266 | L-sorbose | - | builds acid from |
65353 | 65328 | L-xylose | - | builds acid from |
65353 | 17716 | lactose | - | builds acid from |
65353 | 25097 | lyxose | - | builds acid from |
65353 | 17306 | maltose | - | builds acid from |
65353 | 17306 | maltose | + | builds acid from |
65353 | 29864 | mannitol | + | builds acid from |
65353 | 37684 | mannose | - | builds acid from |
65353 | 6731 | melezitose | - | builds acid from |
65353 | 28053 | melibiose | - | builds acid from |
65353 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
65353 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
65353 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
65353 | 506227 | N-acetylglucosamine | - | builds acid from |
65353 | 17632 | nitrate | - | reduction |
65353 | 64681 | phenyl beta-D-glucuronide | - | hydrolysis |
65353 | potassium 2-dehydro-D-gluconate | - | builds acid from | |
65353 | potassium 5-dehydro-D-gluconate | + | builds acid from | |
65353 | 32032 | potassium gluconate | - | builds acid from |
65353 | 32032 | potassium gluconate | + | assimilation |
65353 | 16634 | raffinose | - | builds acid from |
65353 | 17814 | salicin | - | builds acid from |
65353 | 30911 | sorbitol | - | builds acid from |
65353 | 28017 | starch | - | builds acid from |
65353 | 28017 | starch | - | hydrolysis |
65353 | 17992 | sucrose | - | builds acid from |
65353 | 17992 | sucrose | + | builds acid from |
65353 | 27082 | trehalose | - | builds acid from |
65353 | 27082 | trehalose | + | builds acid from |
65353 | 27897 | tryptophan | - | energy source |
65353 | 32528 | turanose | - | builds acid from |
65353 | 53424 | tween 20 | + | hydrolysis |
65353 | 53423 | tween 40 | - | hydrolysis |
65353 | 53425 | tween 60 | - | hydrolysis |
65353 | 53426 | tween 80 | - | hydrolysis |
65353 | 17151 | xylitol | - | builds acid from |
65353 | 18222 | xylose | - | builds acid from |
metabolite production
- @ref: 65353
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 65353
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
65353 | tryptophan deaminase | - | 4.1.99.1 |
65353 | catalase | + | 1.11.1.6 |
65353 | esterase (C 4) | + | |
65353 | esterase Lipase (C 8) | + | |
65353 | leucine arylamidase | + | 3.4.11.1 |
65353 | chymotrypsin | + | 3.4.4.5 |
65353 | alpha-glucosidase | + | 3.2.1.20 |
65353 | lipase (C 14) | - | |
65353 | valine arylamidase | - | |
65353 | alpha-galactosidase | - | 3.2.1.22 |
65353 | beta-galactosidase | - | 3.2.1.23 |
65353 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
65353 | alpha-mannosidase | - | 3.2.1.24 |
65353 | alpha-fucosidase | - | 3.2.1.51 |
65353 | cytochrome oxidase | - | 1.9.3.1 |
65353 | urease | - | 3.5.1.5 |
Isolation, sampling and environmental information
isolation
- @ref: 65353
- sample type: hydrothermal sediments (depth of 1851m)
- sampling date: 2015-06
- geographic location: Manus Basin
- country: Pacific Ocean
- latitude: 3.73
- longitude: 151.68
- enrichment culture: marine agar
- enrichment culture composition: (l-1): peptone, 5.0 g; yeast extract, 1.0 g; Fe(III) citrate, 0.1 g; NaCl, 19.45 g; MgCl2, 5.98 g; Na2SO4, 3.24 g; CaCl2, 1.8 g; KCl, 0.55 g; Na2CO3, 0.16 g; KBr, 0.08 g; SrCl2, 0.034 g; H3BO3, 0.022 g; Na2SiO3, 0.004 g; NaF, 0.0024 g; (NH4)NO3, 0.0016 g; Na2HPO4, 0.008 g; agar 15.0 g.
- enrichment culture duration: 3 days
- enrichment culture temperature: 50
- isolation procedure: 1.0 g of the sediment sample was suspended with 3 mm sterile seawater, and a 100 µl suspension was spread on marine agar plates. Incubation for 3 days at 50°C under aerobic conditions.
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Hydrothermal vent |
#Environmental | #Aquatic | #Sediment |
Sequence information
16S sequences
- @ref: 65353
- description: 16S rRNA gene sequence
- accession: MF582328
- database: nuccore
GC content
- @ref: 65353
- GC-content: 47.6
- method: high performance liquid chromatography (HPLC)
External links
@ref: 65353
culture collection no.: KCTC 33882, CCTCC AB 2017019
Reference
@id | authors | title | doi/url | journal |
---|---|---|---|---|
20215 | D.Gleim, M.Kracht, N.Weiss et. al. | Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since | http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.html | |
65353 | Qing-lei Sun, Chao Yu, Zhen-dong Luan, Chao Lian, Yong-hua Hu, Li Sun | Description of Bacillus kexueae sp. nov. and Bacillus manusensis sp. nov., isolated from hydrothermal sediments | 10.1099/ijsem.0.002594 | IJSEM 68: 829-834 2018 |