Strain identifier

BacDive ID: 157974

Type strain: Yes

Species: Prosthecomicrobium hirschii

Strain Designation: 16

Strain history: <- J. T. Staley, Univ. of Washington, Seattle, USA; 16

NCBI tax ID(s): 665126 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 64547

BacDive-ID: 157974

DSM-Number: 16181

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, motile

description: Prosthecomicrobium hirschii 16 is a mesophilic, motile bacterium that was isolated from Muskrat Pond,.

NCBI tax id

  • NCBI tax id: 665126
  • Matching level: species

strain history

  • @ref: 64547
  • history: <- J. T. Staley, Univ. of Washington, Seattle, USA; 16

doi: 10.13145/bacdive157974.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Hyphomicrobiaceae
  • genus: Prosthecomicrobium
  • species: Prosthecomicrobium hirschii
  • full scientific name: Prosthecomicrobium hirschii Staley 1984
  • synonyms

    • @ref: 20215
    • synonym: Prosthecodimorpha hirschii

@ref: 64547

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Hyphomicrobiaceae

genus: Prosthecomicrobium

species: Prosthecomicrobium hirschii

full scientific name: Prosthecomicrobium hirschii Staley 1984

strain designation: 16

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes95.774
6948099.997negative

Culture and growth conditions

culture medium

  • @ref: 64547
  • name: MMB MEDIUM (DSMZ Medium 1417)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1417
  • composition: Name: MMB MEDIUM (DSMZ Medium 1417) Composition: Agar 15.0 g/l Glucose 1.0 g/l MgSO4 x 7 H2O 0.565714 g/l Ammonium Sulfate 0.25 g/l Nitrilotriacetic acid 0.190476 g/l Peptone 0.15 g/l Yeast extract 0.15 g/l CaCl2 x 2 H2O 0.063619 g/l ZnSO4 x 7 H2O 0.00104762 g/l FeSO4 x 7 H2O 0.00047619 g/l EDTA 0.000238095 g/l Ammonium Molybdate 0.00017619 g/l MnSO4 x 7 H2O 0.000146667 g/l Thiamine HCl 5e-05 g/l Riboflavin 5e-05 g/l D-Calcium pantothenate 5e-05 g/l CuSO4 x 5 H2O 3.80952e-05 g/l Co(NO3)2 x 6 H2O 2.38095e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l Na2B4O7 x 10 H2O 1.71429e-05 g/l Vitamin B12 1e-06 g/l Distilled water

culture temp

  • @ref: 64547
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69480no99.979
69481no94

Isolation, sampling and environmental information

isolation

  • @ref: 64547
  • sample type: Muskrat (Botany) Pond,
  • geographic location: Chapel Hill, North Carolina
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Pond (small)
#Host#Mammals#Rodentia (Other)

taxonmaps

  • @ref: 69479
  • File name: preview.99_9635.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_4675;97_5666;98_7120;99_9635&stattab=map
  • Last taxonomy: Prosthecomicrobium hirschii subclade
  • 16S sequence: HM037994
  • Sequence Identity:
  • Total samples: 4904
  • soil counts: 439
  • aquatic counts: 4069
  • animal counts: 192
  • plant counts: 204

Safety information

risk assessment

  • @ref: 64547
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 64547
  • description: Prosthecomicrobium hirschii ATCC:27832 16S ribosomal RNA gene, partial sequence
  • accession: HM037994
  • length: 1463
  • database: ena
  • NCBI tax ID: 665126

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Prosthecomicrobium hirschii 16GCA_001305515scaffoldncbi665126
66792Prosthecomicrobium hirschii strain 16665126.4wgspatric665126
66792Prosthecomicrobium hirschii ATCC 278322502376840draftimg665126
66792Prosthecomicrobium hirschii 162648501921draftimg665126

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno94no
motileyes84.584no
flagellatedno70.53no
gram-positiveno98.428no
anaerobicno95.175no
aerobicyes83.079no
halophileno94.449no
spore-formingno94.582no
glucose-utilyes90.945no
thermophileno95.303yes
glucose-fermentno90.122no

External links

@ref: 64547

culture collection no.: DSM 16181, ATCC 27832

straininfo link

  • @ref: 110823
  • straininfo: 39204

literature

  • topic: Genetics
  • Pubmed-ID: 26586892
  • title: Draft Genome Sequence of Prosthecomicrobium hirschii ATCC 27832T.
  • authors: Daniel JJ, Givan SA, Brun YV, Brown PJ
  • journal: Genome Announc
  • DOI: 10.1128/genomeA.01355-15
  • year: 2015

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
64547Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-16181Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16181)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
110823Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID39204.1