Strain identifier

BacDive ID: 141039

Type strain: Yes

Species: Paralkalibacillus indicireducens

Strain Designation: Bps-1

Strain history: T. Tamura and I. Yumoto; AIST, Japan; Bps-1.

NCBI tax ID(s): 1920268 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 8.1 (current version)

General

@ref: 43919

BacDive-ID: 141039

keywords: 16S sequence, Bacteria, facultative anaerobe, halophilic, spore-forming, Gram-positive, motile, rod-shaped, colony-forming

description: Paralkalibacillus indicireducens Bps-1 is a facultative anaerobe, halophilic, spore-forming bacterium that forms circular colonies and was isolated from indigo fermentation liquor used for dyeing, produced from sukumo obtained from craft centre in Data City, Hokkaido, Japan.

NCBI tax id

  • NCBI tax id: 1920268
  • Matching level: species

strain history

  • @ref: 67770
  • history: T. Tamura and I. Yumoto; AIST, Japan; Bps-1.

doi: 10.13145/bacdive141039.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Paralkalibacillus
  • species: Paralkalibacillus indicireducens
  • full scientific name: Paralkalibacillus indicireducens Hirota et al. 2017

@ref: 43919

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Paralkalibacillus

species: Paralkalibacillus indicireducens

strain designation: Bps-1

type strain: yes

Morphology

cell morphology

  • @ref: 43919
  • gram stain: positive
  • cell length: 1.1-2.0 µm
  • cell width: 0.5-0.8 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 43919
  • colony size: 0.5-2.0 mm
  • colony color: creamy-white
  • colony shape: circular
  • incubation period: 3 days
  • medium used: PYG-2 medium

Culture and growth conditions

culture medium

  • @ref: 43919
  • name: PYG-2 medium
  • growth: yes
  • composition: 8 g/L peptone, 3 g/L yeast extract, 10 g/L D-glucose, 1 ml/L metal mixture, 10 g/L Na2CO3, pH 10

culture temp

@refgrowthtypetemperaturerange
43919positivegrowth18-40
43919positiveoptimum33mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
43919positiveoptimum9-10alkaliphile
43919positivegrowth8-11

Physiology and metabolism

oxygen tolerance

  • @ref: 43919
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 43919
  • spore description: spherical endospores at the terminal position in the not swollen sporangia
  • type of spore: endospore
  • spore formation: yes

halophily

@refhalophily levelsaltgrowthtested relationconcentration
43919halophilicNaClpositivegrowth0-10 %(w/v)
43919NaClpositiveoptimum7 %(w/v)

observation

@refobservation
43919quinone MK-7
67770quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4391927613amygdalin-builds acid from
4391918305arbutin-builds acid from
4391917057cellobiose-builds acid from
4391917108D-arabinose-builds acid from
4391918333D-arabitol-builds acid from
4391928847D-fucose-builds acid from
4391912936D-galactose-builds acid from
4391917634D-glucose-builds acid from
4391916024D-mannose-builds acid from
4391916443D-tagatose-builds acid from
4391965327D-xylose-builds acid from
4391916813galactitol-builds acid from
4391917113erythritol-builds acid from
4391928066gentiobiose-builds acid from
4391924265gluconate-builds acid from
4391917268myo-inositol-builds acid from
4391915443inulin-builds acid from
4391930849L-arabinose-builds acid from
4391918403L-arabitol-builds acid from
4391917266L-sorbose-builds acid from
4391965328L-xylose-builds acid from
4391917716lactose-builds acid from
4391917306maltose-builds acid from
4391929864mannitol-builds acid from
43919320061methyl alpha-D-glucopyranoside-builds acid from
4391926546rhamnose-builds acid from
4391930911sorbitol-builds acid from
4391928017starch-builds acid from
4391927082trehalose-builds acid from
4391917151xylitol-builds acid from
43919casein-hydrolysis
4391962968cellulose-hydrolysis
439195291gelatin-hydrolysis
4391928017starch-hydrolysis
4391953424tween 20-hydrolysis
4391953423tween 40-hydrolysis
4391953425tween 60-hydrolysis
4391953426tween 80-hydrolysis
4391937166xylan-hydrolysis
4391917632nitrate-reduction
43919168082-dehydro-D-gluconate+builds acid from
43919581435-dehydro-D-gluconate+builds acid from
4391915824D-fructose+builds acid from
4391962318D-lyxose+builds acid from
4391916988D-ribose+builds acid from
4391916443D-tagatose+builds acid from
4391928053melibiose+builds acid from
4391974863methyl beta-D-xylopyranoside+builds acid from
4391916634raffinose+builds acid from
4391917992sucrose+builds acid from
4391932528turanose+builds acid from

enzymes

@refvalueactivityec
43919catalase+1.11.1.6
43919cytochrome oxidase+1.9.3.1
43919urease+3.5.1.5
43919esterase (C 4)+
43919esterase Lipase (C 8)+
43919leucine arylamidase+3.4.11.1
43919chymotrypsin+3.4.4.5
43919acid phosphatase+3.1.3.2
43919naphthol-AS-BI-phosphohydrolase+
439196-phospho-beta-galactosidase+3.2.1.85
43919alkaline phosphatase-3.1.3.1
43919lipase (C 14)-
43919valine arylamidase-
43919cystine arylamidase-3.4.11.3
43919trypsin-3.4.21.4
43919alpha-galactosidase-3.2.1.22
43919beta-glucuronidase-3.2.1.31
43919alpha-glucosidase-3.2.1.20
43919beta-glucosidase-3.2.1.21
43919N-acetyl-beta-glucosaminidase-3.2.1.52
43919alpha-mannosidase-3.2.1.24
43919alpha-fucosidase-3.2.1.51

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
43919indigo fermentation liquor used for dyeing, produced from sukumo (composted Polygonum indigo leaves) obtained from craft centre in Data City, Hokkaido, JapanData City, HokkaidoJapanJPNAsia42.4708140.875sukumo/extract medium15 ml sukumo hydrolysate, 2 g indigo carmine, 15 g gellan gum in 885 ml distilled water, pH 102 days27
67770Indigo fermentation liquor from a craft centre in Data CityHokkaidoJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Biodegradation#Composting
#Environmental#Microbial community
#Host#Plants#Herbaceous plants (Grass,Crops)

taxonmaps

  • @ref: 69479
  • File name: preview.99_182849.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_67440;97_87626;98_119113;99_182849&stattab=map
  • Last taxonomy: Paralkalibacillus indicireducens subclade
  • 16S sequence: LC197841
  • Sequence Identity:
  • Total samples: 187
  • soil counts: 78
  • aquatic counts: 28
  • animal counts: 53
  • plant counts: 28

Sequence information

16S sequences

  • @ref: 43919
  • description: 16S rRNA gene sequence
  • accession: LC197841
  • database: nuccore

GC content

  • @ref: 67770
  • GC-content: 40.3
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43919

culture collection no.: JCM 31808, NCIMB 15080

literature

  • topic: Phylogeny
  • Pubmed-ID: 28905696
  • title: Paralkalibacillus indicireducens gen., nov., sp. nov., an indigo-reducing obligate alkaliphile isolated from indigo fermentation liquor used for dyeing.
  • authors: Hirota K, Nishita M, Matsuyama H, Yumoto I
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002248
  • year: 2017
  • mesh: Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Coloring Agents, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Fermentation, *Indigo Carmine, Japan, Nucleic Acid Hybridization, *Phylogeny, Polygonum, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43919Kikue Hirota, Masatoshi Nishita, Hidetoshi Matsuyama, Isao YumotoParalkalibacillus indicireducens gen., nov., sp. nov., an indigo-reducing obligate alkaliphile isolated from indigo fermentation liquor used for dyeing10.1099/ijsem.0.002248IJSEM 67: 4050-4056 201728905696
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/