General

@ref: 42395

BacDive-ID: 139787

DSM-Number: 102139

keywords: 16S sequence, Bacteria, thermophilic

description: Rubrobacter spartanus HPK2-2 is a thermophilic bacterium that was isolated from soil adjacent to a volcano caldera.

NCBI tax id

• NCBI tax id: 1933902
• Matching level: species

strain history

• @ref: 42395
• history: <- J. Norman, Plant Biology, Michigan State University; HPK2-2

doi: 10.13145/bacdive139787.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/actinomycetota
• domain: Bacteria
• phylum: Actinomycetota
• class: Rubrobacteria
• order: Rubrobacterales
• family: Rubrobacteraceae
• genus: Rubrobacter
• species: Rubrobacter spartanus
• full scientific name: Rubrobacter spartanus Norman et al. 2017

@ref: 42395

domain: Bacteria

phylum: Actinobacteria

class: Rubrobacteria

order: Rubrobacterales

family: Rubrobacteraceae

genus: Rubrobacter

species: Rubrobacter spartanus

full scientific name: Rubrobacter spartanus Norman et al. 2017

strain designation: HPK2-2

type strain: yes

Culture and growth conditions

culture medium

• @ref: 42395
• name: RUBROBACTER CARBOXYDOVORANS MEDIUM (DSMZ Medium 1606)
• growth: yes
• link: https://mediadive.dsmz.de/medium/1606
• composition: Name: RUBROBACTER CARBOXYDOVORANS MEDIUM (DSMZ Medium 1606) Composition: Agar 15.0 g/l Na2HPO4 x 2 H2O 4.5 g/l KH2PO4 1.5 g/l Tryptone 1.0 g/l Yeast extract 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.01 g/l CaCl2 x 2 H2O 0.01 g/l Ferric ammonium citrate 0.005 g/l H3BO3 0.0009 g/l CoCl2 x 6 H2O 0.0006 g/l ZnSO4 x 7 H2O 0.0003 g/l MnCl2 x 4 H2O 9e-05 g/l Na2MoO4 x 2 H2O 9e-05 g/l NiCl2 x 6 H2O 6e-05 g/l CuCl2 x 2 H2O 3e-05 g/l Distilled water

culture temp

• @ref: 42395
• growth: positive
• type: growth
• temperature: 50
• range: thermophilic

Isolation, sampling and environmental information

isolation

• @ref: 42395
• sample type: soil adjacent to a volcano caldera
• geographic location: Hawaii Volcanoes National Park
• country: USA
• origin.country: USA
• continent: North America

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_87588.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_408;96_36352;97_46201;98_60527;99_87588&stattab=map
• Last taxonomy: Rubrobacter spartanus subclade
• 16S sequence: KY441442
• Sequence Identity:
• Total samples: 33
• soil counts: 19
• aquatic counts: 3
• animal counts: 7
• plant counts: 4

Safety information

risk assessment

• @ref: 42395
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 42395
• description: Rubrobacter spartanus 16S ribosomal RNA gene, partial sequence
• accession: KY441442
• length: 1395
• database: ena
• NCBI tax ID: 1933902

External links

@ref: 42395

culture collection no.: DSM 102139, LMG 29988

straininfo link

• @ref: 96230
• straininfo: 400147

literature

• topic: Phylogeny
• Pubmed-ID: 28875901
• title: Rubrobacter spartanus sp. nov., a moderately thermophilic oligotrophic bacterium isolated from volcanic soil.
• authors: Norman JS, King GM, Friesen ML
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijsem.0.002175
• year: 2017
• mesh: Actinobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hawaii, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry, Volcanic Eruptions
• topic2: Transcriptome

Reference