Strain identifier

BacDive ID: 134233

Type strain: Yes

Species: Companilactobacillus crustorum

Strain history: CIP <- 2007, CCUG

NCBI tax ID(s): 392416 (species)

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General

@ref: 32030

BacDive-ID: 134233

keywords: genome sequence, 16S sequence, Bacteria, facultative aerobe, Gram-positive, rod-shaped

description: Companilactobacillus crustorum CCUG 53174 is a facultative aerobe, Gram-positive, rod-shaped bacterium that was isolated from Sourdough.

NCBI tax id

  • NCBI tax id: 392416
  • Matching level: species

strain history

@refhistory
416722007, CCUG
67770LMG 23699 <-- I. Scheirlinck R-27957.
118794CIP <- 2007, CCUG

doi: 10.13145/bacdive134233.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Companilactobacillus
  • species: Companilactobacillus crustorum
  • full scientific name: Companilactobacillus crustorum (Scheirlinck et al. 2007) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus crustorum

@ref: 32030

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus crustorum

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
32030positive8.5 µm0.75 µmrod-shapedno
118794positiverod-shapedno

colony morphology

  • @ref: 59818
  • incubation period: 1 day

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41672MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
118794CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
32030positivegrowth15-45
32030positiveoptimum30mesophilic
41672positivegrowth37mesophilic
59818positivegrowth30mesophilic
67770positivegrowth30mesophilic
118794positivegrowth10-37
118794nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
32030facultative aerobe
59818aerobe
59818anaerobe
118794facultative anaerobe

spore formation

  • @ref: 32030
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
32030NaClpositivegrowth5.0-7.0 %
32030NaClpositiveoptimum6 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3203017057cellobiose+carbon source
3203028757fructose+carbon source
3203028260galactose+carbon source
3203017234glucose+carbon source
3203017716lactose+carbon source
3203017306maltose+carbon source
3203037684mannose+carbon source
32030506227N-acetylglucosamine+carbon source
3203026546rhamnose+carbon source
3203017814salicin+carbon source
3203027082trehalose+carbon source
11879417632nitrate-reduction
11879416301nitrite-reduction
11879417632nitrate+respiration

metabolite tests

  • @ref: 118794
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
118794oxidase-
118794alcohol dehydrogenase-1.1.1.1
118794catalase-1.11.1.6
118794lysine decarboxylase-4.1.1.18
118794ornithine decarboxylase-4.1.1.17
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118794-+---+----++---+++--

Isolation, sampling and environmental information

isolation

@refsample typesampling dategeographic locationcountryorigin.countrycontinent
59818Sourdough2004-06-08KalkenBelgiumBELEurope
67770Wheat sourdough
118794Food, Wheat sourdoughOost VlaanderenBelgiumBELEurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Fermented

taxonmaps

  • @ref: 69479
  • File name: preview.99_1676.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_954;97_1113;98_1324;99_1676&stattab=map
  • Last taxonomy: Companilactobacillus
  • 16S sequence: AM285450
  • Sequence Identity:
  • Total samples: 4649
  • soil counts: 475
  • aquatic counts: 523
  • animal counts: 3246
  • plant counts: 405

Safety information

risk assessment

  • @ref: 118794
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
32030Lactobacillus crustorum 16S rRNA gene, type strain LMG 23699TAM2854501519nuccore392416
67770Lactobacillus crustorum JCM 15951 gene for 16S ribosomal RNA, partial sequenceLC4808121505ena392416

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Companilactobacillus crustorum LMG 23699GCA_001438825scaffoldncbi392416
66792Companilactobacillus crustorum NBRC 107159GCA_007992035contigncbi392416
66792Lactobacillus crustorum JCM 159511423737.3wgspatric1423737
66792Lactobacillus crustorum strain LMG 23699392416.3wgspatric392416
66792Lactobacillus crustorum strain NBRC 107159392416.14wgspatric392416
66792Lactobacillus crustorum LMG 236992663763277draftimg392416
66792Lactobacillus crustorum JCM 159512695420765draftimg1423737
67770Companilactobacillus crustorum JCM 15951GCA_001434585contigncbi1423737

GC content

@refGC-contentmethod
3203035.5
6777035-36high performance liquid chromatography (HPLC)
6777035genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno98.474yes
gram-positiveyes95.538yes
anaerobicno82.619no
aerobicno96.294no
halophileyes92.127yes
spore-formingno96.808yes
glucose-utilyes92.814yes
motileno94.033no
thermophileno99.816no
glucose-fermentyes89.014no

External links

@ref: 32030

culture collection no.: CCUG 53174, CIP 109613, LMG 23699, JCM 15951, NBRC 107159

straininfo link

  • @ref: 91827
  • straininfo: 133809

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Biotechnology33360296Lemon juice and apple juice used as source of citrate and malate, respectively, enhance the formation of buttery aroma compounds and/or organic acids during Type 2 and Type 3 sourdough productions performed with Companilactobacillus crustorum LMG 23699.Comasio A, Van Kerrebroeck S, De Vuyst LInt J Food Microbiol10.1016/j.ijfoodmicro.2020.1090202020Bread/analysis/*microbiology, Citric Acid/*metabolism, Citrus/chemistry, Fermentation, Flour/microbiology, *Food Microbiology, Fruit/chemistry, *Fruit and Vegetable Juices, Lactobacillus/*metabolism, Malates/metabolism, Malus/chemistry, Odorants/analysisMetabolism
Phylogeny33502298Companilactobacillus pabuli sp. nov., a lactic acid bacterium isolated from animal feed.Jung JY, Kang HK, Jin HM, Han SS, Kwon YC, Eun JJ, Kim SC, Seo MJ, Ryu BG, Chung EJInt J Syst Evol Microbiol10.1099/ijsem.0.0046702021

Reference

@idauthorstitledoi/urlID_cross_referencepubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
32030Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172828128776041
41672Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7376
59818Curators of the CCUGhttps://www.ccug.se/strain?id=53174Culture Collection University of Gothenburg (CCUG) (CCUG 53174)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
91827Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID133809.1
118794Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109613Collection of Institut Pasteur (CIP 109613)