Strain identifier

BacDive ID: 132446

Type strain: Yes

Species: Nesterenkonia aurantiaca

Strain Designation: CK5

Strain history: CIP <- 2013, A. Poli, CNR ICB, Pozzuoli, Italy: strain CK5

NCBI tax ID(s): 1436010 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 24235

BacDive-ID: 132446

DSM-Number: 27373

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-positive, coccus-shaped, colony-forming

description: Nesterenkonia aurantiaca CK5 is an aerobe, Gram-positive, coccus-shaped bacterium that forms circular colonies and was isolated from soil.

NCBI tax id

  • NCBI tax id: 1436010
  • Matching level: species

strain history

@refhistory
24235<- A. Poli, Istituto di Chimica Biomolecolare, Pozzuoli, Napoli; CK5
67770A. Poli; Natl. Res. Counc. (C.N.R.), Inst. of Biomol. Chem. (I.C.B.), Italy; CK5.
116553CIP <- 2013, A. Poli, CNR ICB, Pozzuoli, Italy: strain CK5

doi: 10.13145/bacdive132446.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Nesterenkonia
  • species: Nesterenkonia aurantiaca
  • full scientific name: Nesterenkonia aurantiaca Finore et al. 2016

@ref: 24235

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Pseudomonadales

family: Micrococcaceae

genus: Nesterenkonia

species: Nesterenkonia aurantiaca

full scientific name: Nesterenkonia aurantiaca Finore et al. 2016

strain designation: CK5

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell shapemotilityconfidence
43734positive1.5 µmcoccus-shapedno
69480no93.906
69480positive100
116553positivecoccus-shapedno

colony morphology

  • @ref: 43734
  • colony size: 1 mm
  • colony color: Orange
  • colony shape: circular
  • incubation period: 2 days
  • medium used: Solid medium A

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43734Solid medium Ayes
24235BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
24235HORIKOSHI-1 MEDIUM (DSMZ Medium 1081)yeshttps://mediadive.dsmz.de/medium/1081Name: HORIKOSHI-1 MEDIUM (DSMZ Medium 1081) Composition: Agar 15.0 g/l Glucose 10.0 g/l Na2CO3 10.0 g/l Yeast extract 5.0 g/l Polypeptone 5.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.2 g/l Distilled water
116553CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
43734positivegrowth4-30
43734positiveoptimum25mesophilic
24235positivegrowth25mesophilic
67770positivegrowth25mesophilic

culture pH

@refabilitytypepHPH range
43734positivegrowth6.5-10alkaliphile
43734positiveoptimum9

Physiology and metabolism

oxygen tolerance

  • @ref: 43734
  • oxygen tolerance: aerobe

spore formation

@reftype of sporespore formationconfidence
43734endosporeno
69481no100
69480no99.995

halophily

@refsaltgrowthtested relationconcentrationhalophily level
43734NaClpositivegrowth0.003-20 %(w/v)
43734NaClpositiveoptimum2 %(w/v)slightly halophilic

observation

  • @ref: 67770
  • observation: quinones: MK-7, MK-8

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4373416024D-mannose-assimilation
4373415740formate-assimilation
4373429806fumarate-assimilation
4373424265gluconate-assimilation
4373430849L-arabinose-assimilation
4373416634raffinose-assimilation
4373415824D-fructose-builds acid from
4373417634D-glucose-builds acid from
4373465327D-xylose-builds acid from
4373428260galactose-builds acid from
4373417716lactose-builds acid from
4373417992sucrose-builds acid from
4373427082trehalose-builds acid from
4373417561L-cysteine-energy source
4373415971L-histidine-energy source
4373417191L-isoleucine-energy source
4373418019L-lysine-energy source
4373416643L-methionine-energy source
4373417115L-serine-energy source
4373416414L-valine-energy source
4373485146carboxymethylcellulose-hydrolysis
43734casein-hydrolysis
437345291gelatin-hydrolysis
43734606565hippurate-hydrolysis
4373428044phenylalanine-hydrolysis
4373428017starch-hydrolysis
4373453426tween 80-hydrolysis
4373416199urea-hydrolysis
4373417561L-cysteine-nitrogen source
4373415971L-histidine-nitrogen source
4373417191L-isoleucine-nitrogen source
4373418019L-lysine-nitrogen source
4373416643L-methionine-nitrogen source
4373417115L-serine-nitrogen source
4373416414L-valine-nitrogen source
4373417632nitrate-reduction
4373416301nitrite-reduction
4373430089acetate+assimilation
4373416947citrate+assimilation
4373415792malonate+assimilation
4373417272propionate+assimilation
4373430031succinate+assimilation
4373415824D-fructose+carbon source
4373417634D-glucose+carbon source
4373465327D-xylose+carbon source
4373428260galactose+carbon source
4373417716lactose+carbon source
4373417992sucrose+carbon source
4373427082trehalose+carbon source
4373415824D-fructose+energy source
4373417634D-glucose+energy source
4373465327D-xylose+energy source
4373428260galactose+energy source
4373416977L-alanine+energy source
4373417716lactose+energy source
4373417992sucrose+energy source
4373427082trehalose+energy source
4373417309pectin+hydrolysis
4373437166xylan+hydrolysis
4373416977L-alanine+nitrogen source

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.
4373417076streptomycinyesyes25 µg (disc)
4373428368novobiocinyesyes30 µg (disc)
4373427902tetracyclineyesyes30 µg (disc)
4373471321fusidateyesyes10 µg (disc)
437346472lincomycinyesyes15 µg (disc)
4373428001vancomycinyesyes30 µg (disc)
4373417698chloramphenicolyesyes10 µg (disc)
437347660nystatinyesyes100 Unit (disc)
4373428669bacitracinyesyes10 Unit (disc)
4373417334penicillinyesyes2 Unit (disc)

metabolite production

@refChebi-IDmetaboliteproduction
4373415688acetoinno
4373435581indoleno

metabolite tests

  • @ref: 43734
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
43734catalase+1.11.1.6
43734cytochrome oxidase-1.9.3.1
43734urease-3.5.1.5
43734lipase (C 14)+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeisolation date
24235soilCape King (73° 05' S 166° 40'E)AntarcticaATAAustralia and Oceania-73.0833166.667
43734Soil samplesCape KingAntarcticaATAAustralia and Oceania-73.083166.666
67770Soil from Cape KingAntarcticaATAAntarctica
116553Environment, Soil, lakeCape king lakeAntarcticaATAAntarctica2006-06-08

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_2896.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_1530;97_1826;98_2239;99_2896&stattab=map
  • Last taxonomy: Nesterenkonia
  • 16S sequence: HG795012
  • Sequence Identity:
  • Total samples: 3970
  • soil counts: 791
  • aquatic counts: 600
  • animal counts: 2130
  • plant counts: 449

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
242351Risk group (German classification)
1165531Risk group (French classification)

Sequence information

16S sequences

  • @ref: 24235
  • description: Nesterenkonia sp. DSM 27373 partial 16S rRNA gene, strain DSM 27373, isolate CK5
  • accession: HG795012
  • length: 1481
  • database: ena
  • NCBI tax ID: 1436010

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Nesterenkonia aurantiaca strain DSM 273731436010.5wgspatric1436010
66792Nesterenkonia aurantiaca DSM 273732802428805draftimg1436010
67770Nesterenkonia aurantiaca DSM 27373GCA_004364585scaffoldncbi1436010

GC content

@refGC-contentmethod
4373464.8
2423564.8fluorimetric
6777064.8thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno91.347yes
gram-positiveyes94.567no
anaerobicno99.435no
halophileyes83.153no
spore-formingno93.549yes
thermophileno94.299yes
glucose-utilyes91.185no
aerobicyes96.857yes
flagellatedno97.125yes
glucose-fermentno88.218no

External links

@ref: 24235

culture collection no.: DSM 27373, JCM 19723, CIP 110620

straininfo link

  • @ref: 90784
  • straininfo: 406146

literature

  • topic: Phylogeny
  • Pubmed-ID: 26813578
  • title: Nesterenkonia aurantiaca sp. nov., an alkaliphilic actinobacterium isolated from Antarctica.
  • authors: Finore I, Orlando P, Di Donato P, Leone L, Nicolaus B, Poli A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000917
  • year: 2016

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24235Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-27373Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27373)
43734Ilaria Finore, Pierangelo Orlando, Paola Di Donato, Luigi Leone, Barbara Nicolaus, Annarita PoliNesterenkonia aurantiaca sp. nov., an alkaliphilic actinobacterium isolated from Antarctica10.1099/ijsem.0.000917IJSEM 66: 1554-1560 201626813578
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
90784Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID406146.1
116553Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110620Collection of Institut Pasteur (CIP 110620)