Strain identifier

BacDive ID: 12795

Type strain: No

Species: Pseudomonas aeruginosa

Strain Designation: PAO1

Strain history: <- M. Schobert, Inst. Microbiology, TU Braunschweig, Germany; PAO1 <- S. Lory, Univ. of Washington, Seattle, USA <- ? <- <- P. Rountree

NCBI tax ID(s): 287 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8364

BacDive-ID: 12795

DSM-Number: 19880

keywords: human pathogen, mesophilic, Bacteria

description: Pseudomonas aeruginosa PAO1 is a mesophilic human pathogen that was isolated from human, infected wound.

NCBI tax id

  • NCBI tax id: 287
  • Matching level: species

strain history: <- M. Schobert, Inst. Microbiology, TU Braunschweig, Germany; PAO1 <- S. Lory, Univ. of Washington, Seattle, USA <- ? <- <- P. Rountree

doi: 10.13145/bacdive12795.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas aeruginosa
  • full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacterium aeruginosum

@ref: 8364

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas aeruginosa

full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900

strain designation: PAO1

type strain: no

Culture and growth conditions

culture medium

  • @ref: 8364
  • name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)
  • growth: yes
  • link: https://bacmedia.dsmz.de/medium/381
  • composition: Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water

culture temp

  • @ref: 8364
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836916199urea+hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase+3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
8364---++-+-+--++-+++++-+
8364---++-+-+--++-+++++-+

Isolation, sampling and environmental information

isolation

  • @ref: 8364
  • sample type: human, infected wound

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host#Human
#Host Body-Site#Other#Wound

Safety information

risk assessment

  • @ref: 8364
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

External links

@ref: 8364

culture collection no.: DSM 19880

literature

  • topic: Pathogenicity
  • Pubmed-ID: 34989245
  • title: Characterization and in vitro testing of newly isolated lytic bacteriophages for the biocontrol of Pseudomonas aeruginosa.
  • authors: Harada LK, Silva EC, Rossi FP, Cieza B, Oliveira TJ, Pereira C, Tomazetto G, Silva BB, Squina FM, Vila MM, Setubal JC, Ha T, da Silva AM, Balcao VM
  • journal: Future Microbiol
  • DOI: 10.2217/fmb-2021-0027
  • year: 2022
  • mesh: *Bacteriophages/genetics, Host Specificity, In Vitro Techniques, Myoviridae/genetics, *Pseudomonas aeruginosa

Reference

@idauthorscataloguedoi/urltitle
8364Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19880)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19880
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68369Automatically annotated from API 20NE