Strain identifier

BacDive ID: 10473

Type strain: Yes

Species: Neisseria meningitidis

Strain Designation: 21, M 1027

Strain history: CIP <- 1973, ATCC <- S.E. Branham, Maryland, USA: strain M 1027 <- C. Miller, Chicago, USA: strain 21

NCBI tax ID(s): 487 (species)

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General

@ref: 3758

BacDive-ID: 10473

DSM-Number: 10036

keywords: microaerophile, Bacteria, 16S sequence, genome sequence, mesophilic

description: Neisseria meningitidis 21 is a microaerophile, mesophilic Proteobacterium that was isolated from spinal fluid from fatal case of meningitis.

NCBI tax id

  • NCBI tax id: 487
  • Matching level: species

strain history

<- ATCC <- S. E. Branham, M 1027 <- C. Miller, 21
CIP 73.10 <-- ATCC 13077 <-- S. E. Branham; USA; M 1027 <-- C. Miller; USA; No. 21.

doi: 10.13145/bacdive10473.20211221.6

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/proteobacteria
  • domain: Bacteria
  • phylum: Proteobacteria
  • class: Betaproteobacteria
  • order: Neisseriales
  • family: Neisseriaceae
  • genus: Neisseria
  • species: Neisseria meningitidis
  • full scientific name: Neisseria meningitidis (Albrecht and Ghon 1901) Murray 1929 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Micrococcus meningitidis

@ref: 3758

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Neisseriales

family: Neisseriaceae

genus: Neisseria

species: Neisseria meningitidis

full scientific name: Neisseria meningitidis (Albrecht and Ghon 1901) Murray 1929

strain designation: 21, M 1027

type strain: yes

Morphology

colony morphology

  • @ref: 3758
  • type of hemolysis: gamma
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3758COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://bacmedia.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
37616MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and NeisseriayesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)

culture temp

@refgrowthtypetemperaturerange
3758positivegrowth37mesophilic
37616positivegrowth37mesophilic
44624positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 44624
  • oxygen tolerance: microaerophile

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837727897tryptophan-energy source
6837716199urea-hydrolysis
6837718257ornithine-degradation
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
6836935581indoleno

metabolite tests

@refChebi-IDmetaboliteindole test
6837735581indole-
6836935581indole-

enzymes

@refvalueactivityec
3758catalase+1.11.1.6
3758cytochrome-c oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377beta-galactosidase-3.2.1.23
68377alkaline phosphatase-3.1.3.1
68377lipase-
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
3758------+/--------------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
3758-+-+-------+-
3758-+-+-------+-
3758-+-+/--------+-
3758-+---------+/--
3758-+-+-------+-
3758-+/--+/-+/-------+-
3758+/--+/-+/-------+--
3758+---------+/---
3758+-+/--------+--
3758+-+-------+--
3758+-+-------+--
3758+-+-------+--
3758-+-+-------+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling date
3758spinal fluid from fatal case of meningitisChicagoUSAUSANorth America41.8818-87.6232
44624Human cerebrospinal fluid,fatal meningitisChicagoUSAUSANorth America1937
67770Spinal fluid from fatal case of meningitisChicago, ILUSAUSANorth America

isolation source categories

Cat1Cat2Cat3
#Infection#Disease#Meningitis
#Host Body Product#Fluids#Cerebrospinal fluid

Safety information

risk assessment

  • @ref: 3758
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Neisseria meningitidis 16S rRNA gene, partialAJ247244457ena487
3758N.meningitideis gene for 16S rRNAX74900716ena487

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Neisseria meningitidis NCTC10025GCA_900638555completencbi487
66792Neisseria meningitidis strain NCTC10025487.2792completepatric487

External links

@ref: 3758

culture collection no.: DSM 10036, ATCC 13077, NCTC 10025, CCUG 3269, CIP 73.10, JCM 33544

straininfo link

@refpassport
20218http://www.straininfo.net/strains/161789
20218http://www.straininfo.net/strains/96711
20218http://www.straininfo.net/strains/96714

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3758Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10036)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10036
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37616Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10662
44624Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 3269)https://www.ccug.se/strain?id=3269
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/