Title
Conformational rearrangements enable iterative backbone 𝑁-methylation in RiPP biosynthesis
Published Date
2021-07-30
Authors
Group
Freeman Lab University of Minnesota
Author Contact
Freeman, Michael F. (mffreema@umn.edu)
Type
Dataset
Experimental Data
Simulation Data
Abstract
The data deposited here are raw files, R code and mass spec, associated with the results presented in the paper, "Conformational rearrangements enable iterative backbone N-methylation in RiPP biosynthesis." These data have been made publicly available in keeping with the journal's data availability policy.
Description
R Code:
Supplementary Data 1. Code for running kinetics simulations
File name: Supplementary Data 1.R
Mass Spectrometry .raw files:
Supplementary Figure 4a-c. Mass spectrometric analysis of split borosin coexpressions.
File name: 20200110_FSM1167_SonA+SonM_24.5min.raw
Supplementary Figure 4d-j. Mass spectrometric analysis of split borosin coexpressions.
File name: 20200909_KC1063_StrA+StrM_15min_redalk_18pm4.raw
Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and
compared to kinetic model simulations.
File names:
20200220_FSM1178_kineticmodeling_t=2.raw
20200220_FSM1179_kineticmodeling_t=2.raw
20200220_FSM1180_kineticmodeling_t=8.raw
20200220_FSM1181_kineticmodeling_t=8.raw
20200220_FSM1182_kineticmodeling_t=20.raw
20200220_FSM1183_kineticmodeling_t=20.raw
20200220_FSM1184_kineticmodeling_t=40.raw
20200220_FSM1185_kineticmodeling_t=40.raw
20200220_FSM1186_kineticmodeling_t=60.raw
20200220_FSM1187_kineticmodeling_t=60.raw
Supplementary Figure 25a. Mass spectrometric analysis of SonM mutant in vitro
reactions.
File name: 20190701_kc1007_His-SonA_Y93F.raw
Supplementary Figure 25b. Mass spectrometric analysis of SonM mutant in vitro
reactions.
File name: 20190515_fsm1155_his-SonA-sonMT-R67K.raw
Supplementary Figure 25c. Mass spectrometric analysis of SonM mutant in vitro
reactions.
File name: 20190515_fsm1154_his-sonA_sonMTR67A.raw
Supplementary Figure 25d. Mass spectrometric analysis of SonM mutant in vitro
reactions.
File name: 20190701_kc1008_His-SonA_Y58F.raw
Supplementary Figure 25e. Mass spectrometric analysis of SonM mutant in vitro
reactions.
File name: 20190515_fsm1156_his-sonA-SonMT-Y71F.raw
Supplementary Figure 25f. Mass spectrometric analysis of SonM mutant in vitro
reactions.
File name: 20190701_kc1009_His-SonA_DBLMUT.raw
Funding information
Sponsorship:
National Institutes of Health (R35 GM133475 to M.F.F.) and the University of Minnesota along with the BioTechnology Institute (M.F.F., M.H.E., W.A.H.)
Referenced by
Miller, F. S., Crone, K. K., Jensen, M. R., Shaw, S., Harcombe, W. R., Elias, M. H., & Freeman, M. F. (2021). Conformational rearrangements enable iterative backbone N-methylation in RiPP biosynthesis. Nature communications, 12(1), 1-14.
License
CC0 1.0 Universal
Suggested Citation
Miller, Fredarla S.; Crone, Kathryn K.; Jensen, Matthew R.; Shaw, Sudipta; Harcombe, William R.; Elias, Mikael H.; Freeman, Michael F..
(2021). Conformational rearrangements enable iterative backbone 𝑁-methylation in RiPP biosynthesis.
Retrieved from the Data Repository for the University of Minnesota,
https://doi.org/10.13020/y8ry-gm18.