SYNTHESIS, CHARACTERIZATION AND ANTI-MICROBIAL ACTIVITY OF 4(8-HYDROXYQUINOLIDEN-5-YL)-2-(SUBSTITUTED PHENYL) OXAZOL-5-ONE.

Various Schiff bases (3a-e) were prepared by condensation reaction of 8hydroxy-5-quinoline carboxaldehyde 1 and diffrent substituted hippuric acids (2a-e). The schiff Bases (3a-e) were then reacted with acetic anhydride in sodium acetate and yielded 4-(8-hydroxy quiolinden-5-yl)-2-substi-tuted phenyl oxazolone. All the oxazolone derivatives were characterized by elemental analysis, spectral studies and antimicrobial activities, One of the oxazolone (4b) was then treated wiht transition metal salts and yield the metal chelates. All the metal chelates of 4b were characterized by metal; ligand (M:L) ratio, magnetic moment, reflectance spectra and anti-microbial activities.

carboxaldehyde (0.01 mole) and substituted' huppuric acid (2a-e) (0.01 mole) in THF (50 ml) was added by triethylamine (1 ml).The mixture was kept aside with stirring for 5 hrs. at room temperature. The resultant mixture poured in cold water and acidified with dil. HCI. The greenish yellow salt was filtered off, washed with THF and then with H20 and air dried. All the Schiff bases are directly used for the synthesis of oxazolones i.e. 4a-e.

Synthesis of 4-(8-hydroxy quinoliden-5-yl)-2-phenyloxazol-5aid (4a-e):
To a solution of Schiff Base derivative (30-e) in ethanol and acetic anhydride at stoichiometric ratio and few ml of HAc was refluxed for 12 hours. The resultant mixture was added into cold water and the precipitates were obtained by neutralization of the mixture by dilute alkali, The product was then filtered off, washed by water and air dried. The details about all the (4a-e) derivatives are given in Table-1.

Synthesis of metal Chelates of 4b
To solution of 4b (0.02 mole) in formic acid water mixture metal acetate solution (0.01 mole) in water was added dropwise under stirring at room temperature. The mixture was then added by solid sodium acetate till the completion of precipitates of metal chelate. It was then filtered, washed by water

INTRODUCTION
Perusal of the literature reveals that the oxazolone derivatives are found to be active parmacological agents [1][2][3][4][5][6][7] . Most of these oxazolone rings contain phenyle rings. Hitherto there is no report been found in literature about the oxazolone derivative containing well known chelating agent say 8-hydroxy quiniline (i.e. oxine). If this compound is introduced into such oxazolone derivative it may change the biological activity upto some extent. With this point of. view, the work containing schiff base based oxazolone derivatives has been undertaken. Hence the present communication deals with the synthesis, characterization and antimcrobial activities of (8-hydroxy quinoliden-5-yl)-2-aryl axazol-5-one. The chelating properties of one of the derivative (i.e. 4b) is also summarized. The research work is shown in Scheme -1.

Materials
All the chemical pertaining to this research work have been obtained from Alza chemicals, Nadiad.

Synthesis of Schiff Bases
To a well mechanical stirred and cooled suspension of 8-hydroxy-5-quinoline and air dried. The detailed analysis of metal chelates of 4b are summarized in Table -2.

Measurements
Elemental analysis of all the compound were performed on C, H, N and O elemental analyzer. IR specttra were recorded in KBr pellets on parkin Elmer IR spectrohotometer and H' NMR Spectra in CDCI3 or DMSO were scanned on FTNMR spectrophotometer. Metal contents of all the metal chelates were estimated by known method 8 . Magnetic suscetibility measurement of all the meta chelates were carried out at room temerature by the guoy method, mercury tetra thiocynato cobalt (II) HG[Co(NCS)4] was used as a calibrant. The diffuse reflectance attachment MgO was employed as the reference compound.
The antifungal activity of all the copounds and chelates was carried out by the method repored earlier 9 . The plant pathogens listed in Table 3 have been selected for the study. One of the significant difference to e expected is the presence of more briadened band in the region of 3300-35-cm -1 of metal chelate as the oxygen of the O-H ligand has formed

RESULTS AND DISCUSSIONS
All the oxazolone derivatives are in the form of amorphous yellow to dark brown powder. The