Removing Heavy Metals from Pharmaceutical Effluent by Pelargonium grandiflorum

This investigation was carried out to determine the accumulation of heavy metals in Pelargonium grandiflorum grown in chemical and toxicology laboratories’ pharmaceutical effluent and wastewater irrigated soil in the vicinity of sewage treatment plant (STP), Pharmacy Faculty, Tehran. The results revealed that wastewater was highly rich in plant nutrients and heavy metals. The wastewater irrigation significantly (P<0.05/ P<0.01) increased the contents of heavy metals in the soil and plants grown in wastewater irrigated soil. The enrichment of various metals were recorded in the order of Fe >Zn > Pb >Cu > Cd > Cr > Mn in these plants. Moreover contents of different heavy metals in the different parts of plants such as root, young and old leaves and stems showed significant (P<0.05) and positive correlation with contents of Cd (r = +82 to r = +96), Cr (r = +74 to r = +94), Cu (r = +84 to r = +98), Fe (r = +88 to r = +98), Mn ( r = +80 to r = +96), Pb (r = +74 to r = +96) and Zn (r = +88 to r = +98) in the wastewater irrigated soil. Although, the contents of Cd, Cu, Fe, Mn, Pb and Zn in soil after growing of Pelargonium grandiflorum after 60 days were recorded within the prescribed limit of WHO/FAO standards. Pelargonium (Grandiflorum) has shown ability to extract lead, Cr (III) , Cr(VI) and Cd from contaminated soils; about 35.9 %, 41.9% , 41.7% and 29.8% respectively in the root and leaves zone. To quantify the occurrence and the distribution of heavy metals and to prevent them from passing through wastewater collection and treatment systems into soil and ground water bodies represents an urgent task for applied environmental sciences in the coming years. Public acceptance of green technologies is generally higher than that of industrial processes. The responsible organizations should stimulate research to upgrade existing waste water treatment by implementing phytoremediation modules and demonstrating their reliability to the public.


INTRODUCTION
There is a lack of toxicity studies for various groups of chemicals, for example: pharmaceuticals 1- 3 , nano-particles [4][5] and industrial chemicals [6][7][8] .This has resulted in difficulties when performing risk assessments since any risk assessment relies on the availability of reliable and relevant studies.Pharmaceuticals were first identified to pose environmental risks in the 1990s, and since then the number of available monitoring and effect studies has increased steadily.0][11] Thus far, there are several examples of APIs convincingly shown to cause effects on organisms in the environment.
Pharmaceuticals are synthetic or natural chemicals that can be found in prescription medicines, overthe-counter therapeutic drugs and veterinary drugs which contain active ingredients that have been designed to have pharmacological effects and confer significant benefits to society.They include painkillers, birth control pills, tranquilizers and antidepressants 12 among many others.Waste from pharmaceuticals fall in the category of emerging toxicants and pollutants.These pollutants are currently undergoing a regularization process although the directives and legal frameworks are not set-up yet 13 .Pharmaceuticals find their way into the environment via human and animal excreta from disposal into the sewage system 14 .Their presence in water can also be attributed to pharmaceutical industry waste, hospital waste and therapeutic drugs 15 .They are not only released into the environment after use.Some are disposed during manufacture or as unused or expired drugs 16 .For phytoextraction to function, contaminants must be bioavailable (ready to be absorbed by roots).Bioavailability depends on metal solubility in soil solution.Only metal ions in soil water and in exchangeable positions are readily available for plant uptake.Some metals, such as Zn and Cd, occur primarily in exchangeable form; i.e. readily bioavailable form.Others, such as Pb, occur as precipitates; a considerably less bioavailable form.Therefore, Pb is very difficult to remove.The capacity of a soil to adsorb Pb increases with increasing pH, cation exchange capacity, organic carbon content, soil/water ratio and phosphate levels [17][18] .Plants show some ability to reduce the hazards of organic pollutants [19][20][21] , the greatest progress in phytoremediation has been made with metals [22][23][24] .Pelargonium grandiflorum is an erect straggling herbaceous shrub, usually growing to a height of 0.75 m.The glaucous stem is soft smooth and shiny.The foliage is attractive, smooth and glaucous.The dull grayish green leaves are deeply palmately lobed, usually 5 cm long and 8 cm wide.The leaf margins are coarsely toothed and some of the leaves have a brownish zonal marking.The fairly large and beautiful flowers vary from creamishwhite to pink with darker blotches on the upper two petals.Pelargonium grandiflorum belongs in the family Geraniaceae, a large cosmopolitan family of approximately 11 genera and 800 species in subtropical and temperate regions of the world.The South African genera in the Geraniaceae family are Monsonia, Sarcocaulon, Pelargonium, Erodium and Geranium.There are approximately 270 species of Pelargonium which occur in S, E and NE Africa, Asia, St Helena, Tristan da Cunha, Madagascar, Australia and New Zealand, most of which (± 219 species) occur in southern Africa.Pelargoniums are often wrongly called geraniums 25- 27 .Pelargonium grandiflorum will be horticulturally rewarding and easily adaptable to any garden situation.Before planting in the garden plenty of compost to the soil should be added.The plant pelargonium could be as part of a shrubby border or in a large rockery or even around a small pond.Due to being adapted and growing in any condition we select this plant for phytoremediation study.
The aim of the current study is to: (1) Assess the applicability of Pelargonium Grandiflorum in removing heavy metals from the contaminated soil after and (2) determine the accumulation of heavy metals in Pelargonium grandiflorum grown in chemical and toxicology laboratories' pharmaceutical effluent and wastewater irrigated soil in the vicinity of sewage treatment plant (STP), Pharmacy Faculty, Tehran.

MATERIALS AND METHOD
Effluent samples were collected between 1 April and October 5th, 2015.Sample collection containers (1 L, amber glass) were washed in hot water, rinsed three times with distilled water, rinsed three times with acetone, and then baked in a heated oven at 250ºC for a minimum of four hours.A 24-hour composite sample (500 mL of effluent) was collected by WWTP operators from each WWTP, using their own equipment, and 100 2 mL of a solution containing 5.0 g/L of Na2EDTA and 25 mg/L of ascorbic acid was added at the time of collection.The samples were shipped overnight on wet ice, and stored at 4°C until extraction.Because of the large number of sampling sites and chemical analytes, it was logistically too difficult and expensive to collect and analyze field blanks as well as duplicates from each location.Field blanks were collected from 20% of the sampling sites, with the field blanks being prepared from laboratory distilled water that was transferred into sampling containers and preserved at the time of collection.
Duplicates were collected and analyzed for 10% of the sample sites.

Waste water Effluent
Effluents from five educational and research laboratories in pharmaceutical sciences branch, Azad university in Tehran, including, Food Science and Technology research (Effluent 1&2), Toxicology (Effluent 3,4), Analytical chemistry(Effluent 5) were used in this study.Effluent 1 and Effluent 2 were from the same laboratory but were collected on separate occasions with a 3 week time interval.Although Effluent 1 and Effluent 2 come from the same WWTP, they were treated as 2 different effluents due to the variability of their characteristics.This difference is attributed to the significant experiments which occurred following the first sampling event.After collection, the effluent was immediately transported to the research laboratory for analysis.Physico-chemical parameters such as pH, Electrical Conductivity, Total Solids, Total Dissolved Solids, Total hardness, Chloride, Sulphate, Dissolved oxygen, , Calcium, Sodium, Cadmium, Lead, Zinc, Copper, Chrome, Manganese ,Iron and Potassium were analyzed as per the standard methods 28 .
The initial concentration of heavy metals/ metalloid in the plants and effluents were analyzed before introduction into the studied soil samples.After 10days of treatment up to 60 days in every ten days , final concentration of heavy metals/metalloid in effluent samples and plants were analyzed using Atomic Absorption Spectroscopy .The samples were analyzed by an Atomic Absorption Spectrophotometer Model AA-6200 (Shimadzu, Japan) using an air-acetylene flame for heavy metals and using at least five standard solutions for each metal.All necessary precautions were taken to avoid any possible contamination of the sample as per the AOAC guidelines [29][30][31] .The efficiency of plant in accumulating heavy metals in their areal parts such as leaf, stem and also root were calculated using bio-concentration factor.

Estimation of Heavy Metals in Effluents
The heavy metals/metalloid in the effluent samples such as Chrome, Zinc, Copper, lead, cadmium, Manganese and Iron were analyzed before and after treatment by AAS after digestion of plant materials by AOAC method [31][32] .The plant samples were washed in deionized water dried (24 hrs at 80°C) immediately to stabilize the tissue and stop enzymatic reactions.After drying, samples were ground to pass a 1.0mm screen using the appropriate Wiley Mill.After grinding, the sample were thoroughly mixed and a 5-to 8-g aliquot withdrawn for analyses and storage 33 .Weighed 0.5 to 1.0 g of dried (80°C) plant material that has been ground (0.5 to 1.0 mm) and thoroughly homogenized and place in a tall-form beaker or digestion tube.Added 5.0 ml concentrated HNO 3 (65 %) and cover beaker with watch glass or place a funnel in the mouth of digestion tube and allow to stand overnight or until frothing subsides.Place covered beaker on hot plate or digestion tube into block digester and heat at 125°C for 1 hour.Removed the digestion tube and allowed cooling.Added 1 to 2 ml 30% H 2 O 2 and digest at the same temperature.Repeated heating and 30% H 2 O 2 additions until digest is clear.Add additional HNO 3 as needed to maintain a wet digest.After sample digest is clear, removed watch glass and lowered temperature to 80°C.Continued heating until near dryness.Added dilute HNO 3 (10%), and deionized water to dissolve digest residue and bring sample to final volume 34 .

Total dissolved solids (TDS)
The total solid concentration in waste effluent represents the colloidal form and dissolved species.The probable reason for the fluctuation of value of total solid and subsequent the value of dissolved solids due to content collision of these colloidal particles.The rate of collision of aggregated process is also influenced by PH of these effluents 34 .

Chemical oxygen demand (COD)
The chemical oxygen demand test (COD) determines, the oxygen required for chemical oxidation of organic matter with the help of strong chemical oxidant.The COD is a test which is used to measure pollution of domestic and industrial waste.The waste is measure in terms of equality of oxygen required for oxidation of organic matter to produce CO2 and water.It is a fact that all organic compounds with a few exceptions can be oxidizing agents under the acidic condition.COD test is useful in pinpointing toxic condition and presence of biological resistant substances.For COD determination samples were preserved using H2SO4 and processed for COD determination after the entire sampling operation was complete [34][35] .

Biochemical oxygen demand (BOD)
For BOD, 5 samples were immediately processed after Collection for the determination of initial oxygen and incubated at 20 °C for 5 days for the determination of BOD5 [34][35][36] .

Chlorides
Chlorides are generally present in natural water.The presence of chloride in the natural water can be attributed to dissolution of salts deposits discharged of effluent from chemical industries, oil well operations, sewage discharge of effluent from chemical industries, etc 35 .

Sulphates
Sulphate in one of the major cation occurring in natural water.Sulphate being a stable, highly oxidized, soluble form of sulphur and which is generally present in natural surface and ground waters.Sulphate itself has never been a limiting factor in aquatic systems.The normal levels of sulphate are more than adequate to meet plants need [35][36][37][38] .

Bioconcentration Factor
The bio concentration factor is a measure of bioaccumulation of heavy metals.It can be calculated by dividing the trace element concentration in plant tissues (ppm) at harvest by initial concentration of the element in the external nutrient solution (ppm) [38][39] .

Statistical Analysis
For testing statistical significance, student's t-test (SPSS-20) was used.Independent sample t-test was used for finding the mean difference of each parameter control with plants.

RESULTS
Chemical extraction of the soil profile before adding specified amounts of heavy metals is shown in the table 1.Data is averages of the effluent profiles.
The minimum and maximum values of the total solid concentration ranged between 1988-2927 The averaged values ranged between 2044-2356 mg/L for the effluent are well within the maximum permissible limits of 3500 mg/L and according to standards are from U.S. Environmental Protection Agency, the dissolved solids concentration is classified as follows: fresh, 0-1,000 mg/L; slightly saline, 1,000-3,000 mg/L; moderately saline, 3,000-10,000 mg/L; very saline, 10,000-35,000 mg/L; and briny, more than 35,000 mg/L 37- 39 .
As compared to BOD, COD was very high which is normal for effluent of such pharmaceutical laboratories.The minimum and maximum values ranged between 1039-8022 and the averaged values ranged between 1955-3029 mg/L for the studied effluent.In the present study the values of sulphate for untreated effluent was 720 mg/l which was within the permissible limits of 1000 mg/ L according to the WHO standards 38,39 .
This investigation was carried out to determine the accumulation of heavy metals in Pelargonium grandiflorum grown in chemical and toxicology laboratories' pharmaceutical effluent and wastewater irrigated soil in the vicinity of sewage treatment plant (STP), Pharmacy Faculty, Tehran.Results in figures 1,2 and 3 showed significant differences in Cr (III) , Cr(VI) and Cd up taking by different parts of plant.The best results for uptake of Nickel, cadmium and chrome was in the soil with  to r = +96), Pb (r = +74 to r = +96) and Zn (r = +88 to r = +98) in the wastewater irrigated soil.Although, the contents of Cd, Cu, Fe, Mn, Pb and Zn in soil after growing of Pelargonium grandiflorum after 60 days were recorded within the prescribed limit of WHO/FAO standards.
At the end of the test period (60 days ) , the plants were taken out of the soil and separated into their main parts; stems, leaves and roots.Each part of the plant was dried and the moisture content was determined.The contaminant was extracted from each part of the plant by using wet digestion method.Lead content in leaves and root in comparison of contaminated soil by pharmaceutical effluent is shown in figure 4. Pelargonium (Grandiflorum) has shown ability to extract lead, Cr (III) , Cr(VI) and Cd from contaminated soils; about 35.9 %, 41.9% , 41.7% and 29.8% respectively in the root and leaves zone.

DISCUSSION
Kavitha et al. studied the physicochemical analysis of pharmaceutical industrial effluent and treatment plant's efficiency and found the variation in wastewater characteristics from the inlet point to the outlet point of septic tanks 40 .They observed reduction in the following parameters: TSS 4,300-94 mg/l, TDS 2,846-1,308 mg/l, COD 7,280-9.9mg/l, BOD 4,132-6.6 mg/l, chlorides 1,000-300 mg/ l, sulphates 500-300 mg/l and pH between 7.43 and 7.14.Das et al. studied the control of pharmaceutical effluent parameters through bioremediation 1 .They collected the samples from nine different points situated in the industry and observed the range of sulphates 44-1,527 mg/l, TDS 484-1,452 mg/l, total suspended solids 24-84 mg/l and COD 1,257.9-1,542.9mg/l.Madukasi et al. characterized the pharmaceutical wastewater and observed the concentration in mg/l for total suspended solids 425 ± 2.3, total dissolved solids 1,600 ± 1.1, total nitrogen 533.7,BOD 146.7 ± 0.3, Zn 0.056, iron 2.1, Mn 0.605, Cu 0.022, acetic acid 422.7, propionic acid 201.3 and butyric acid 304.5 42 .A suspended growth photobioreactor employing the wild strain of purple nonsulphur photosynthetic bacterium Rhodobacter sphaeroides was utilized to treat the wastewater.The strain was found to be effective in ameliorating hazardous pollutants found in wastewater with over 80 % COD reduction.The strain shows the potential to improve the treatment process and may also be harvested and find use as SCP after further investigation 42 .
Gome and Upadhyay utilized ozone for treatment of pharmaceutical wastewater, which required 32.73 mg/l ozone under acidic condition, whereas under alkaline conditions 30 mg/l ozone was needed 43 .They reported that ozonation can improve biodegradability of wastewater at alkaline pH and higher treatment time favoured the enhanced biodegradability of wastewater.Farhadi et al. used electrocoagulation, photoelectrocoagulation, peroxi-electrocoagulation and peroxi-photoelectrocoagulation processes for the removal of COD from pharmaceutical wastewater originating from Osvah Pharmaceutical Company 44 .To´th et al. used distillation and membrane filtration process for treatment of PIWW, which contains high chemical oxygen demand and adsorbable organically bound halogens (AOX) 45 .The distillation was capable of reducing volatile chemical oxygen demand (VOC-COD) and AOX, while the membrane filtration process was beneficial for the treatment of the bottom product of rectification to concentrate the non-volatile pollutants, reducing the COD values close to the emission limits 45 .In 1988 Mayabhate et al. studied and reported the physicochemical and biological treatment of pharmaceutical wastewater 46 .For physicochemical study, they used ferrous sulphate, ferric chloride and alum as coagulants and for biological treatment activated sludge process was used in an oxidation ditch.Sirtori et al. in 2009, reported that pharmaceutical industrial wastewater contained nalidixic acid (an antibiotic pertaining to the quinolone group), which cannot be easily biodegraded 47 .The biodegradability of nalidixic acid was achieved by the chemical oxidation process followed by biological treatment.Chemical oxidation (photoFenton) enhances the biodegradability, followed by biological treatment using immobilized biomass reactor (IBR).The combined efficiency of treatment was over 95 %, of which 33 % corresponded to the solar photochemical process and 62 % to the biological process.
Phytoremediation of wastewater is an emerging low-cost technique for removal of hazardous metal ions from industrial wastewater and is still in an experimental stage.Heavy metals such as cadmium and lead are not easily absorbed by microorganisms.In such case, phytoremediation proves a better treatment tool for bio-treatment because natural plants or transgenic plants are able to bioaccumulate these toxins 48 .Aquatic plants have excellent capacity to reduce the level of toxic metals, BOD and total solids from the wastewater.Billore et al. in 2001 carried out the treatment of industrial effluent with the help of plants Typha latipholia and Phragmitis karka 49 .This treatment eventually led to COD, BOD, total solids and phosphorus content reduction 49 .Some researchers also reported the phytoremediation of phenol from industrial wastewater by peroxidases of tomato hairy root cultures [50][51] .(Gonza´lez et al. 2006).

CONCLUSION
The present investigation shows that the Pelargonium grandiflorum plant is effective and inexpensive adsorbent for the removal of Pb, Cd, Cr(III), and Cr (VI) from contaminated soil by heavy metals.Pelargonium grandiflorum will be horticulturally rewarding in every garden.Pelargoniums are a versatile group of plants and are easily adaptable to any garden situation.Planting this pelargonium as part of a shrubby border or in a large rockery or even around every small pond.It could be use to line pathways or as an edging along the front of our flower bed that contains taller shrubs.Light pruning after flowering will neaten the bush and not only enjoy its beauty but also it could be used for treating contaminated soil and rescue the environment .This research conduct adsorption of heavy metals by its leaves and root and proved that this friendly method should gain more attention and research interest for the removal of heavy metals from contaminated soil due to its surface area, adsorption capacity and plenty abundant in nature must be followed seriously.Also, this research suggests more investigations by other genera and families of costeffective plants.
To quantify the occurrence and the distribution of heavy metals and to prevent them from passing through wastewater collection and treatment systems into soil and ground water bodies represents an urgent task for applied environmental sciences in the coming years.Public acceptance of green technologies is generally higher than that of industrial processes.The responsible organizations should stimulate research to upgrade existing waste water treatment by implementing phytoremediation modules and demonstrating their reliability to the public.

Table 1 : Heavy Metal concentrations in studied effluent Pharmaceutical Laboratories
* = Standard Error