Profile of Liver Function in Mice after High Dose of Psidium guajava Linn . Extract Treatment

Psidium guajava Linn. is a cultivated and herbal medicines from tropical and subtropical areas that extensively consume around the world as a food and folk medicine. Recent study showed that extract of P. guajava increased the number platelets in thrombocytopenia mice model. The purpose of this research is to know the profile of liver function after high dose treatment of P. guajava extract in mice. The test of acute toxicity was based on up and down procedure of OECD 425. The 7 female mice was divided into 3 groups. The first group was taken as a control with distillated water treatment, the second group as a test group with 2000 mg/kg b.w of P. guajava. extract and the third group as a test group with 5000 mg/kg b.w of P. guajava. extract treatment. Blood of all mice was collected to analyze biochemical test, including ALT, AST and Total Bilirubin. We found that the observation of biochemical tests by statistical approach is no differences in levels of ALT, AST and Total Bilirubin from control and both treatment groups (pe” 0.05). The extract of P. guajava is safe for liver function.

The utilization of herbal plants as medicinal product and supplements has growth tremendously over the past three decades.Many researches have adjusted drug discovery, an principle group of complementary and alternative medicine (CAM) therapy, that is from herbal remedy or botanical sources 1,2 .P. guajava is a typically paramount plant that found in tropical and subtropical countries.People worldwide make guava plants as traditional treatment from the fruits, leas, barks, or roots as a source.P. guajava has the potential pharmacologic activities including antioxidant, anti-inflammatory, antiallergy, antimicrobial, antigenotoxic, antiplasmodial, antispasmodic, antidiabetic, cytotoxic, and cardioactive, effects that are used in the autochthonous system of medicine for the remedy or therapy of miscellaneous human ailments, such as wound, sores, bowels problems, and cholera. 3,4revious study showed that the hepatoprotective effect of P. guajava extract by histopathology of the liver and the phospholipid complex analysis in paracetamol-induced hepatotoxicity animal model.The research showed activity of P. guajava extract better than the plain extract. 5ur recent studies showed that P. guajava extract increased the megakaryocytes amount through enhancement of stem cell factor (SCF) protein expression and followed by generating an increase of platelet count on mice model.However, it still needs to know the toxicity of the high dose before proceed to human clinical trial. 6,7oxicology is a part of pharmacology that study the side effects of chemical or drugs in biological system.The purpose of toxicological screening for the new potential drug discovery and the addition of the therapeutic potential of living sources is very essential.The Food and Drug Administration (FDA) states that it is important to find new molecules from the natural sources, such as plants, for pharmacological activity and toxicity potential in animal models. 8his recent investigation will make an observation on the liver.It already well known that liver is the immense, most composite organ in the gastrointestinal tract and has critical function for metabolism include drug metabolisms.The liver incorporate with three systems, which are the biochemical hepatocytic, major hepatic and the reticuloendothelial system. 9Again, within this research, we would like to know profile of liver function in mice after high dose of P. guajava extract treatment.

Collection of P. guajava
The ripe fruits of P. guajava were collected from Dukuh Waluh Village, Purwokerto, Central Java, Indonesia.The sample was substantiated by Laboratorium Sentral Jatinangor, Universitas Padjadjaran.

Preparation of Plant Extract
P. guajava Linn.extract were made according to a previously published method. 6,7riefly, guava fruits washed rigorously, sliced thinly, then macerated with 96% ethanol solvent for 24 hours and stirring occasionally.Then do the filtering using a funnel buncher.The filtral produced from the filtration was concentrated using a rotary evaporator with a temperature of 80 0 C to obtain the result of a concentrated extract and then suspended by using distilled water as needed.The concentrate of guava extract is then stored at 4 0 C for use in the research process.

Animals
Female Swiss Webster (20-30 g) was procured from laboratory of Pharmacology and Therapy, Universitas Padjadjaran.The animals were acclimatized housed for 1 week under animal laboratory conditions.The mice were fed with standardized chow and clean water for drink ad libitum was provided.The study has got the ethical clearance from Health Research Ethics Committee (No. 1104/UN6.C.10/PN/2017), Faculty of Medicine, Universitas Padjadjaran.

Dose of Acute Toxicity Study
Oral acute toxicity test performed as previously describe. 10Briefly, mice were divided into three groups that administered orally to different groups at the high doses of both 2000 and 5000 mg/kg b.w.The sign of toxic symptoms and mortality were observed from all subject for 14 days.The first group was designated as control group and received distilled water, the second group as treatment group and received dose 2000 mg/kg b.w of P. guajava extract (0,2 ml/kg b.w, p.o), and the third group as treatment group and received dose 5000 mg/kg b.w of P. guajava extract (0,2 ml/kg b.w, p.o).All treatments were administered just once and the subject was observed for 14 days after treatment.

Biochemical Examinations
The mice were sacrificed on the fifteen day after treatment by intracutaneuos injection ketamine 5% and the 1 mL blood from cardiac puncture was collected.The blood proceed further with centrifugation to collect the serum.The activities of serum aspartate transaminase (AST), alanine aminotransferase (ALT) and the extent of total bilirubin serum were analyzed with Siemens Dimension Clinical Chemistry System.

Statistical analysis
Data were determined and analyzed using One-Way Anova.The data was parametric and have normal distribution and homogeneous variants.If necessity was not matched, the test would be replaced with alternative non-parametric; Kruskal-Wallis.Furthermore, the data analyzed with Mann Whitney test to know the differences from each group.The differences of data was significant if P < 0.05.

RESULTS
The purpose of this study is to know profile of liver function in mice after high dose of P. guajava extract treatment with analyzing serum from liver enzymes and total bilirubin.Oral acute   1 and 2. We proceed the data with statistical analysis to confirm whether there were a statistically difference or not.We first analyzed the data using the Shapiro-Wilk test and the results were not normally distributed (p < 0.05).
Because the data were not normally distributed, we used a non-parametric test (Kruskal Wallis Test).The result of the Kruskal Wallis test was shown to be no significant, table 1; ALT (p = 0.029); AST (p = 0.029) and table 2; Total Bilirubin (p = 0.657).Our present data in table 1 and 2 showed no significantly difference among control and treatment groups.
Furthermore, we determined whether the differences between the ALT, AST and Total Bilirubin results from each group were statistically significant or not using Mann Whitney test.The result of the Mann Whitney test showed in Table 3.It indicates that there were no significant difference among group 1 and 2; group 1 and 3 and group 2 and 3 for all examination of biochemical test included ALT, AST and Total Bilirubin (p > 0.05).Finally, our observation indicated that high dose of ethanol extract from P. guajava fruits did not exhibit any remarkable alteration of the hepatic function in the healthy mice.

DISCUSSION
The fruits, leaves, barks and roots of P. guajava extract contain alkaloid, flavonoids, tannins and saponin 3 .These phytochemical are known to have several biologic roles in plants, include have pharmacological actions in human such as protect the cells from toxic agent. 11Various studies have showed that extract of P. guajava is safe for liver.Within this study, we also found that ethanol extract of P. guajava fruits is safe for liver function based on the observation of biochemical tests and followed by statistical analysis.
Examination of biochemical test of doses from both 2000 mg/kg b.w and 5000 mg/kg b.w of ethanol extract of P. guajava fruit did not affect ALT, AST and total bilirubin extents from normal mice blood.Other previous study suggested that the -augmenting effect on the hepatocellular function of the extract could be achieve by the action of the varied extract contents, especially the presence of flavonoid which have antioxidative properties that could be as a result of its radical-scavenging activity. 12,13Saponins from extract have a function for depressed the level of blood cholesterol, which may effects in decreasing the metabolic problem in the liver. 14Furthermore, triterpenoid have hepatoprotective effects to significantly block the LPS (lipopolysaccharide) and (d-galactosamine) d-Ga1N-convinced increases in both serum alanine aminotransferase and aspartate aminotransferase extents, exhibiting the nuclear condensation, and improvement others sign for protective effect of the liver. 15,16his could be due to liver damage does not occurs after high dose of P. guajava extract fruits treatment.Therefore, the activities of most enzymes normally detectable in blood remain normal in healthy animal subjects. 13Moreover, one of major liver functions is the biochemical hepatocyte system, which is responsible for the vast majority of all metabolic activities in the body, including drug metabolism as a part of xenobiotic metabolism. 9he limitations in this study we only focus on liver function test since the liver is the main organ in drug metabolism.It is necessary to check the function of other organs such as kidney and brain.

CONCLUSION
The animal models are induced by extract of P. guajava in doses 2000 and 5000 mg/kg b.w with oral feeding which were observed for 14 days did not showed significantly liver function tests (LFTs).It can be concluded that extract of P. guajava is safe.
Published by Oriental Scientific Publishing Company © 2018This is an Open Access article licensed under a Creative Commons license: Attribution 4.0 International (CC-BY).