Influence of Osmotic Pretreatments and Drying Methods on Water Activity of Dried Pear

The aim of this study was to evaluate the impact of pretreatments viz glucose, sucrose, glycerol and drying techniques (sun drying and cabinet drying at 50°C and 60≤C) on water activity of dried pear slices. The concentration of all the osmotic agents was regular (60%). The impact of drying techniques and pretreatments were found measurably critical (p≤0.05). The lowest water activity (aw) was found in cabinet dried pear at 60°C and highest in sun dried pear slices. Samples treated with glycerol reached the lower water activity values than treated with glucose and sucrose.

Pear fruit (PyruscommunisL.)belongs to the family Rosaceae, subfamily Maloideaeor Spiraeoideaeand genus PyrusL 1 .Due to its low caloric and high nutritive value, it is much appreciated among the consumers.Pear fruit is rich in both macronutrients and micronutrients 2 .
Most fruits and vegetables have a definite harvesting time and a limited shelf-life.Harvested fruits deteriorate quickly due to microbial and biochemical activities.However, different techniques are used to extend their shelflife by a few weeks, one year or more 3 .The common processing techniques of pears are, to preserve in syrup, to convert in purees to be used in nectars, yogurts, canningand drying 4 .
Osmotic dehydration is an efficient form of moisture elimination from solid food, inflicting no change in phase of water 5 .In fruits about 50% of their original weight is reduced when subjected to osmotic treatments before drying 6 .It is often carried out as a pre-processing step before foods are subjected to further processing such as air drying 7 , vacuum drying 8 , freezing 9 , freeze drying 10 , sun drying, pasteurisation or acidification and coating by edible surface layers 11 .Besides,osmotic dehydration is used to treat fresh produce to improve sensory, functional and even nutritional properties.
This technique is based on the immersion of fruits in a hypertonic solution.This hypertonic solution affords a higher osmotic pressure and a lower water activity 6 .Water activity a w is a better indication of food perishibility.It is the term that relates to potential of a microbe to grow in aselected surroundings.Removal of water by drying or concentration will lessen its susceptibility to microbial assault 12 .During the osmotic dehydration, three types of mass transfers in countercurrent flux can occur 6 : water flux from the product to the solution; solute transference, from the solution to the product, and natural solutes (organic, mineral sugars, acid, vitamins, etc.) flux, from the fruit to the solution resulting in the removal of water which eventually leads to lower water activity.
Osmotic dehydration of pear can be a useful technique to preserve and obtain new processed products of interest to the consumer.The aim of this study was to evaluate the effect of osmotic dehydration on water activity of pear slices for shelf life extension.

MATERIAL AND METHODS
Two varieties of pear (vicar of winkfield and Bartlett) were selected for the study.Fruits were sorted, washed with water and peeled manually.Both the varieties were sliced to 10mm thickness by power operated slicer and immersed in glucose, sucrose and glycerol solutions for 12 hrs.Following osmotic treatments were applied before drying.T1 control (plain water) T2 pear slices were immersed in 60% Glucose + 0.5% KMS T3 pear slices were immersed in 60% Glucose + 0.5 % Ascorbic acid T4 pear slices were immersed in 60% Glucose + 0.25 % KMS +0.25% Ascorbic acid T5 pear slices were immersed in 60% sucrose + 0.5% KMS T6 pear slices were immersed in 60% sucrose + 0.5% Ascorbic acid T7 pear slices were immersed in 60% sucrose + 0.25 % KMS +0.25% Ascorbic acid T8 pear slices were immersed in 60% Glycerol + 0.5% KMS T9 pear slices were immersed in 60% Glycerol + 0.5% Ascorbic acid T10 pear slices were immersed in 60% Glycerol + 0.25 % KMS +0.25% Ascorbic acid Sun Drying Pretreated samples of both the varieties from all the treatments were spread on alumunium trays (75 x 45 x 6cm) and placed under direct sunlight during day time between 9 am to 5 pm.Samples were given regular turnings to achieve uniform drying.On each day the samples were collected in the evening and kept in perforated and closed polyethylene bags and spread out again on the following day.Samples were dried till the predetermined moisture level of 10-12 per cent was achieved.

Mechanical Drying
Pretreated Pear varieties were loaded on aluminum trays (75 x 45 x 6cm) as described in sun drying.The loaded trays were kept in cabinet drier and drying was conducted at two temperatures levels i.e 50 o C and 60 o C till the predetermined moisture level of 10-12 per cent was achieved.Following drying all the treated samples were packed in polypropylene boxes.Water activity (a w ) was analysed by using water activity meter.

RESULTS AND DISCUSSION
As it is widely known that the stability and safety of foods enhance if the wateractivity of the product decreases.The water activity of foods affects the multiplication, metabolic activity, resistance and survival of the organism present 13 .The reduction of a w to about 0.93 might be enough to suppress the growth of most pathogenic bacteria 14 .Most mould and yeast strains are inhibited between a w of 0.88 and 0.80, even though a few osmophilic yeast lines can nevertheless develop right down to a w of 0.6 15 .
The results from the present study showed that the mechanical drying produced the lowest a w compared to sun drying.Among both the varieties, the lowest wateractivity of0.367was recorded in Bartlett in T8 (Glycerol + KMS and dried in cabinet drier at 60°C) and highest of 0.519was recorded in vicar of winkfield in T1 (control and sundried sample).Effect of drying methods and pretreatments on the water activity values were statistically significant (pd"0.05).
As evident from the Fig 1 and Fig 2 all the treatments resulted in reduction in a w as compared to fresh fruit.The osmotic pretreatments were more efficient in reducing a w as a large amount of water was removed at some stage in osmosis and incorporation of sugars occurred.Similar results were reported in osmotically dehydrated mango 16 .
Fruits treated with glycerol reached lower wateractivity values than those treated with glucose and sucrose.This can be due to glycerol, a polyhydric alcohol, decreasedthe a w by means of hydrogen binding with it 17 .The results were in agreement with the findings of Ayub 18 who reported a decrease in a w of persimmon slices.Panwar et al 19 reported the similar results in aonla segments.

CONCLUSION
It is concluded that the osmotic dehydration is an effective way of lowering the wateractivity of pear slices thus have the extended shelf life even without refrigeration.All the pretreatments resulted in lower water activity values however pear slices treated with glycerol reached the lowest wateractivity values irrespective of the varieties.Further mechanical drying resulted in lower water activity as compared to sundrying.