Authors propose a new technique that combines the laser induced fluorescence (LIF) and particle tracking velocimetry (PTV). We developed new hollow microcapsule tracers by doping temperature sensitive fluorescent dye. This method does not need to dissolve the fluorescent dye to the working fluid and quantitative measurement would be possible regardless of the conditions of working fluid. The hollow microcapsules show large temperature sensitivity of -1.7%/℃ and large oxygen sensitivity. In the present study, we demonstrated the visualization of oxygen distribution in a micro channel using the functional hollow microcapsules.