In this study, we made a device that can perform cell manipulation of oocytes and reagent injection to a single cell. This two-dimensional chip composed of two functions which is; (1) loading oocytes one by one and (2) reagent injection by electrically-induced bubble on a chip. When gene transfer is performed, a pulse voltage is applied to the substrate fabricated on a chip, and the electric field is concentrated to the holes which is composed of dielectric material, and hence the fine electrically-induced bubbles were generated. When the bubbles are collapsed and a pore formation is successfully provided to the target together with the transportation of reagent which performs gene transfer. All chips are fabricated by photolithography targeting the achievement of a high-precision, high-throughput and stable injection system.