This paper describes sequential formation of asymmetric lipid vesicle, which applies pulse jet flow to an asymmetric planar lipid bilayer with multiple lipid components formed by split and contact of droplets. Asymmetric planar lipid bilayers with multiple lipid components were formed by contacting various types of lipid monolayers using movable wells on a revolving table. We successfully produced two types of the asymmetric lipid vesicles containing red or green fluorescent lipids on the outer leaflet with our single device using the proposed method. The asymmetricity of the generated vesicles was evaluated by the distribution of the fluorescence intensities. The proposed device will be applicable for generating artificial cell models with multiple asymmetric lipid components.