In this study, the influence of vibration stimulation to cultured undifferentiated human iPS (induced Pluripotent Stem) cells was investigated. Three-dimensional micro vibration stage was used to stimulate the cultured cells. This stage can excite three-dimensional vibration with total amplitude up to around 30 urn in each (x, y and z) direction. The diameter of mountable culture dish onto the stage is 35 mm. For the experiment, human iPS cells co-cultured with SNL 76/7 cells as feeder cells are provided. By using the stage, the cells were stimulated with horizontal or vertical vibration as a mechanical stimulation. Frequency of the vibration is set at 10 Hz, and total amplitude it is set at 30 um. As a result, there is no significant difference in proliferation rate between stimulated cases and statically cultured case. However, the result suggests that the horizontal vibration stimulation might accelerate proliferation of undifferentiated human iPS cells.