Abstract
Poly(methacrylate)s labeled with perylene diimide (PDI) derivatives were synthesized. The dye moiety was selectively introduced by appropriate molecular design at (1) the chain end, (2) the center segment of the main chain, and (3) the whole chain contour. The bright fluorescence from PDI allows the direct observation of the single polymer chain with a high signal-to-noise ratio. The three-dimensional orientation of the chain segment and the conformation of the polymer chains were successfully observed at the single molecule level.
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The polymerization of poly(iBMA/PDIMA) with Mn ≳ 106 resulted in the broad molecular weight dispersion; therefore, the fractional precipitation was carried out to obtain the labeled poly(iBMA) with the narrow distribution (Mw/Mn = 1.21). A monolayer on water was prepared by spreading a mixed benzene solution of the labeled and unlabeled poly(iBMA) (Mn = 2.1 × 105), respectively, where the fraction of the labeled chain was 0.25%. The monolayer was transfered by a vertical dipping method onto a glass cover slip.
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Aoki, H., Takahashi, T., Tamai, Y. et al. Poly(methacrylate)s Labeled by Perylene Diimide: Synthesis and Applications in Single Chain Detection Studies. Polym J 41, 778–783 (2009). https://doi.org/10.1295/polymj.PJ2009099
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DOI: https://doi.org/10.1295/polymj.PJ2009099
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