Abstract
The purpose of this study is the development of a novel synthetic blocking reagent for the enzymelinked immunosorbent assay (ELISA) method. The water-soluble amphiphilic phospholipid polymer, poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-co-styrene (St)], was synthesized, and the function of the poly(MPC-co-St) as a blocking reagent was compared with conventional blocking reagents which are made of proteins such as bovine serum albumin (BSA) and casein. The poly(MPC-co-St) solution functioned at the same level as BSA solution and casein solution for preventing non-specific antibody adsorption (p>0.01). When the 1.0 wt% BSA solution and 1.0 wt% casein solution were used as a blocking reagent, the remaining activity of the immobilized antibody decreased about 50% after 20 days. On the other hand, in 0.01 wt% and 0.1 wt% poly(MPC-co-St) solutions, the activity remained 76% and 91% of the initial value, respectively. The effects of poly(MPC-co-St) on the stabilization of the immobilized antibody depended on its concentration. These results indicated that the poly(MPC-co-St) had the ability to inhibit denaturation of protein, that is, proteins in the ELISA system kept their native structure. We concluded that the water-soluble amphiphilic poly-(MPC-co-St) is an effective synthetic blocking reagent in the ELISA method.
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Sakaki, S., Iwasaki, Y., Nakabayashi, N. et al. Water-Soluble 2-Methacryloyloxyethyl Phosphorylcholine Copolymer as a Novel Synthetic Blocking Reagent in Immunoassay System. Polym J 32, 637–641 (2000). https://doi.org/10.1295/polymj.32.637
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DOI: https://doi.org/10.1295/polymj.32.637
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