Seasonal Variation of Zooplankton Population with Reference to Water Quality of Iril River in Imphal

The zooplankton population of Iril river of Imphal valley of Manipur was investigated with reference to water quality. The fish biodiversity potential of the river remain intake despite the suburban exposure of the river. Since plankton play a great food chain role for fish community, knowing the population of zooplankton as secondary resource is needed. Deterioration of water quality in urban area remain, in most cases, a basic feature. The present investigation endeavour to establish the influence, if any, of water quality of a river in sub-urban settings to the zooplankton population. Five sites were selected stretching from upstream before the river enter the urban area to the downstream as the river exit the urban area. Since the river is in suburb and for some special physical features the Irilriver maintain a significant volume and there seems to be dilution effect and pollution of the river remain a lesser concern. Nevertheless, the study establishespossible influences of the change in water quality to plankton population.


INTRODUCTION
The potential of rivers as good habitats for ichthyo-fauna seems degraded with onslaught of anthropogenic interference impairing the quality and quantity of river water.The Manipur Central Valley districts are endowed with many wetlands which are often connected with rivers and as such the rivers pose important source of fish resources.The recent changes in water quality impair the potential of the rivers to a significant degree.The Iril river, which is very important for water supply of the Imphal city also cater for the protein food need of the people around with its fish resources.Moreover ecologically the ichthyo-faunalbiodiversity of the river is significant.While most of the other rivers in the Imphal city have lost such inevitable characteristics, the Irilriver still maintain a part of its pristinefeatures.However the onslaught of urban exposures impacts the river and the biotic components.The study on zooplankton population with reference to water quality was carried out.The results may reveal an in-depth inference for better conservation of the river while it is not too late.The river is connected with five very important major wetlands in the valley indirectly and knowledge of its ecological health is a good approach towardsholistic means for overall aquatic ecosystem health in the beautiful valley of Manipur.
Discharge of waste and surface run off causes deleterious effect in floraand fauna and other aquatic organisms (Sahet al.,2000).Water qualities of rivers have beendeteriorated due to disposal of garbage, religious offerings, sewage, recreationaland constructionalactivities inthe catchment areas (Singhet al.,2012).The problem of anthropogenic environmental distortion continuously affects rivers (NanditaChakrabortyet al.,1995).
There is indication that the headwaters to mouth, the physical features vary significantly within a lotic water system and present a continuous gradient of physical variations, which evolves association within biota and other a-biotic features (Pathani and Upadhyay, 2006).Thus the biota of an aquatic ecosystem directly reflects the conditions existing in the environment (Bhattet al., 1984).
Monitoring of zooplankton communities is needed to allow us to predictively model the ecosystem (Deborah and Robert, 2009).The zooplankton are known not only to form an integral part of the lotic community but also contribute significantly to the biological productivity of the fresh water ecosystem (Makarewicz & Likens, 1979).Zooplankton populations can expand in rivers by growth of the suspended organisms (Talling & Rzoska,1967) or by the hatchingof resting eggs in river sediments (Moghraby,1977).However zooplanktons are being transported as river inundates the floodplains because the numerous small lakes loss zooplanktons to the flow ( James and William, 1988 ).

Site description
Manipur lies in the extreme part of the North-East India, a sub-Himalayan hilly state, stretching from 23o50´N -25o41´N and 93o 02´ E -94o48´E.The total geographical area is 22, 327 sq.km of which only about 10% is the enchanting central valley and the rest is surrounding ranges of hills of altitude 800 m to 3000 m above the mean sea level.Thus the central valley comprises 2238 sq.km of which wetlands occupies about 524.51 sq.km.Irilriveris one of the major tributaries of Manipur river system in the central valley district of Manipur.It originates from Lakhamei village of Senapati district near the border with Nagaland with some tributaries originating from Ukhrul district and ultimately flows through the valley to meet Imphalriver in the southern suburb of Imphal city.Five sites were selected starting from the point where the river enter the greater Imphal city area and ending at the conjoining point with Imphal river as the river exit the greater Imphal area to be a part of Manipur river system.The extent of length of the river in the study area is about 20 kilometers.

Methodology
The five sampling sitescomprises two in the upstream to Imphal city, one at Imphal city area and two in the downstream.Analyses were carried out in monthly interval in the year 2012.Studies on seasonal zooplankton population is based on Standard methods of APHA (1989), Adoni (1985), Lackey (1938), Edmondson (1974)and Needham and Needham (1966),.Nylon-bolting net(mesh size; 60-80 µm) was used for collecting zooplankton.Analysis of physico-chemical parameters were done based on standard methods of APHA( 1989) and Trivedy and Goel(1984).For the statistical calculation including ANOVA(Analysis of variance) methods of Parker (1973), Trivedi, Goel and Trisal (1987) and Kothari (2004) were used in computing the analysis.

RESULTS
The results of the studiesare logically classified into two sets namely pre-urban exposure sites and post-urban exposure sites.Means of the sites for the respective category is taken and the results are reproduced as Table 1,2and 3.This categorization presents a vivid account if urban exposure of the river gives impact to water quality and zooplankton population.
The temperature of water for pre-urban sites ranges from 19.98 ± 0.21 0 C in January to 25.42 ± 0.02 0 C in July and for post urban sites it ranges from 20.36 ± 0.19 0 C in January to 25.61±0.16 0C in July.
The mean of P H value increases in the post urban exposure sites in the months ranging from February to May and Septemberto November.There is,however,no significant co-relation of P H with zooplankton population.The value of P H ranges from 7.30± 0.10 in January to 8.42 ± 0.07 in August.In the post-urban sites it ranges from 7.23 ± 0.04in January to 8.56 ± 0.15 in September.There is slight increase in the value of P H with the increase in river volume due to seasonal flood.
The conductivityvalue ranges from 65.00 ± 2.36µ Siemens/ cm² inJanuary to 185.00 ± 2.36 µSiemens/cm² in June for pre-urbansites.It ranges from 71.11±6.94µ Siemens/ cm² in January to 237.78 ± 15.03 µ Siemens/ cm² in June for the posturban sites.There is no significant co-relation of conductivity with zooplankton population.The Total dissolved solids (TDS) value ranges from 41.67±2.35ppm in January to 143.34 ±9.43ppm in August for pre-urban sites and for posturban sites it ranges from 48.89 ± 6.94 ppm in January to 180.00 ± 8.82 ppm in August.It shows negative co-relation with Zooplankton population,r =-0.531, p> 0.05 and significant at 5% level for preurban sites however there is no significant corelation in the post urban sites.
It is important to note that there is no significant co-relation of the physico-chemical parameters with zooplankton population in the posturban sites.
Turbidity ranges from 25.22 ± 0.12 NTU in January to 89.75 ±3.56 NTU in August for pre-urban sites and for post-urban sites it ranges from 31.09 ±3.05 NTU in January to 94.24 ± 1.34 NTU in August and no significant co-relation with zooplankton population.

Dissolved
oxygen(DO) recorded minimum of 5.08 ± 0.00 ppm in March to maximum of 7.61±0.14ppminSeptember for pre-urban sites and minimum of 5.14±0.31ppm in May and maximum of 7.38 ± 0.12 ppm in August for posturban sites.
Free CO 2 ranges from 1.43 ± 0.16ppm in December to 13.97 ± 0.16 ppm in August for preurban sites and for post-urban sites it ranges from 3.01 ± 0.83ppm in December to 18.33 ± 1.27 ppm in August .
Bio-chemical oxygen demand (B.O.D)of pre-urban sites showed negative co-relation with zooplankton population,r=-0.537,p>0.05and significant at5%level.The value ranges from 2.29 ± 0.22ppm in December to 5.79 ± 0.00ppm in August for pre-urban sites and from 2.84 ± 1.07 ppm in December to 7.61± 1.10 ppm in August for posturban sites.
Hardness values for pre-urban sites ranges from 32.00± 2.83 ppm in January to 98.00± 2.83 ppm in August.For post-urban sites the value ranges from 37.33 ±4.16 ppm in Januaryto 123.33 ± 2.31 ppm in August.There is no significant corelation with zooplankton population.
There is no significant co-relation of Nitrate, Inorganic phosphate and Potassium with zooplankton population.
The value of nitrate in pre-urban sites ranges from 0.034 ±0.002 ppm in January to 0.238 ± 0.023 ppm in September.For post-urban sites the value ranges from 0.065 ± 0.034 ppm in January to 0.426 ± 0.084 ppm in September.
Inorganic phosphate content for pre-urban sites ranges from 0.014 ± 0.001 ppm in February to0.042 ± 0.001 ppm in September.Whereas for post-urban sites it ranges from 0.024 ± 0.011 ppm in February to 0.075 ± 0.014 ppm in September.
The value of Potasium for pre-urban sites ranges from 1.17 ±0.23 ppm in October to 5.17 ± 0.23 ppm in August whereas for the post-urban sites it rangesfrom 2.11 ± 0.38 ppm in January to 8.67 ± 0.67 ppm in April.
The Rotifera population count for pre-urban sites ranges from 3.60 ± 0.93 U/L in July to 8.82 ± 0.34 U/Lin April.For post-urban sites it ranges from 4.48 ± 0.35U/L in May to 8.62 ± 1.24 U/L in April.
For population count of Cladocerafor the pre-urban sites, the values rangefrom1.59 ± 0.47U/ L in May to 4.38 ± 0.42 U/L in April.For post-urban sites it ranges from 1.94± 0.27 U/L in May to 5.46 ± 1.00 U/L inApril.
The Copepoda population count for preurban sites ranges from0.84±0.17 U/L in December to 1.77 ± 0.13 U/L inApril.For post-urban sites it ranges from 0.72 ± 0.33 U/L in July to 2.42 ± 0.33 U/L in October.
As a whole, the total zooplankton population for pre-urban sites ranges from 6.48 ± 0.34 U/L in Mayto 14.97 ± 0.21 U/L in April.Thus the primary peak of population growth is in April with a secondary peak in November.For post urban sites, the time of primary and secondary peaks are in similar pattern even as the value of count ranges from 7.36 ± 0.21 U/L inMay to 16.02 ± 0.83 U/L in April.The sudden drop of zooplankton population from April to May is due to early seasonal rain followed by flood.ANOVA (Analysis of variance) is calculated for the three zooplankton groups.For pre-urban sites the calculated value of F-ratio is 76.6 and for post-urban sites the F-ratio is 75.43,both of which are very much greater than the table valueof 3.32 at 5% level with d.f.being v 1 =2and v 2 =33.Thus there is significant difference in sample means for the three groups of zooplanton.It is therefore concluded that the change in populationof zooplankton population during the seasons is highly significant.

Discussions
From the results it can be established that zooplankton population of the river has no significant co-relation with most of the physicochemical parameters except for Total dissolved solids (T.D.S.) and Bio-chemical oxygen demand ( B.O.D.) in the pre-urban sites.The negative corelation may rather be established for decrease in population due to flooding of the river than any possible impact of the values of the parameters.Though the F-ratio for variance of pre-urban to post urban sites of Nitrate ( 5.59 at 5% level with d.f.being v 1 = 1 and v 2 = 22 when the table value is 4.30) and Inorganic phosphate (7.241 at 5% level with d.f.being v 1 = 1 and v 2 = 22 when the table value is 4.30) are significant, these parameters do not affect zooplankton population in pre and post urban exposure since there is no significant variance of the later in the two sets of sites.The change in water quality of the river, with reference to the remaining physico-chemical parameters, shows no significant variance after urban exposure (except for Inorganic phosphate and Nitrate).Though total zooplankton population is negatively co-related with TDS and BOD, the two physico-chemical parameters exhibit no variance in pre-and post-urban exposures, and there is no significant variation of the zooplankton population in the two sets of observations.The peculiar river basin setting of Irilriver in the area, that is natural reservoir like feature, help in dilution of the pollutants specially in two of the posturban sites.It is formed due to confluence of heavily silt loaded streams in the midst.Moreover on account of the rivers situation at the periphery of the main urban area and no particular sewage draining into the river, pollution of the river is at a low level.Plankton densities in unregulated tropical rivers are often low ( James& William,1988).Rzoska(1978) found that reproduction of zooplankton in rivers is rarely observed at velocities in excess of 0.4 m. s -1 .The drop of population in May be due to this reason of increased velocity.Velocity of the river water flow is maximal during flood time.NanditaChakrabortyet al., (1995) established role of nutrient gradient in unequal distribution of plankton species in Hooglyriver.However significant co-relation is not observed in the present studies, which may be due to very low level of nutrient content in the study sites.
Pathani and Upadhyay (2006) reported increase of zooplankton population from winter season and reaching maximum in summer.The present finding is in similar patter except for a slight decline in the month of December.It thereby creates two peaks -one primary peak of zooplankton population in April (Spring) and one secondary peak in November (Autumn).The primary peak could have been shifted to later month of summer season if the arrival of seasonal rain was normal.This season early seasonal rain accompanied with high flood in May could have led to decrease in zooplankton population in May.The bimodal pattern of this zooplankton population peak is grossly in association with findings, ofMathew (1978)and Sinha (1992).Bhatt et al., (1984)reported maximal zooplankton population during the period of minimum velocity.The present peak in April is in association with this observation.Exposure to contaminants can severely impact zooplankton (Bradley and Roberts, 1987) but the insignificant variation of zooplankton populationof pre and post urban exposure sites in the present studies is associated with low pollution level even after the river enter the urban area.

CONCLUSION
As for the present time, pollution level of Irilriver is not alarming and conservation of the river is very much needed for preventing further ecological deterioration, because the water of the river is ultimately needed for water supply of the Imphal city.Moreover Iril is the only river in the Imphal area keeping intake its bio-diversity specially the ichthyo-fauna.Monitoring of zooplankton communities is needed to allow us to predictively model the ecosystem ofIrilriverand it will be helpful in modeling for conservation of the river ecosystem.

Fig. 1 :
Fig.1: Mean zooplankton population of the two sets of sites for Irilriver 2012

Table 1 :Physico-chemical properties of Irilriver in Imphal (average values)
N.B.:Pre-urban-before urban exposure ;Post-urban-after urban exposure

Table 2 : Physico-chemical properties of Irilriver in Imphal (average values)
N.B.:Pre-urban-before urban exposure ;Post-urban-after urban exposure

Table 3 : Zooplankton population count of Iril river in Imphal (average values in U/L)
N.B.:Pre-urban-before urban exposure ;Post-urban-after urban exposure