1993 Volume 46 Issue 6 Pages 507-509
An analytical method for measurement of the synthetic antibacterials, difrazon and morantel, in pork was developed. The study was performed under the following conditions: a Finepak SIL C 18S was used as the analytical column, and the HPLC mobile phase consisted of 40%(v/v) acetonitrile and 0.1%(v/v) orthophosphoric acid in a mixture of 5 mM 1-hexanesulfonic acid sodium salt, pH 2.65. The antibacterials were detected at 380 nm for difurazon and 320 nm for morantel. The calibration curves for authentic difurazon and morantel were linear in the range of 5 to 200 ng. The detection limits were one ng for both antibacterials. The recovery rates of the authentic antibacterials added to pork or kidney tissue were more than 90%.