Optimal Timing for Canine Artificial Insemination with Frozen Semen and Parentage Testing by Microsatellite Markers in Superfecundency

Shigehisa TSUMAGARI; Yasushi ICHIKAWA; Hiroshi TORIUMI; Kakashi ISHIHAMA; Mitsuo MORITA; Misao KANAMAKI; Masatoshi TAKEISHI

doi:10.1292/jvms.65.1003

Shigehisa TSUMAGARI, Yasushi ICHIKAWA, Hiroshi TORIUMI, Kakashi ISHIHAMA, Mitsuo MORITA, Misao KANAMAKI, Masatoshi TAKEISHI

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Keywords: canine, optimal time, parentage testing

JOURNAL FREE ACCESS

2003 Volume 65 Issue 9 Pages 1003-1005

DOI https://doi.org/10.1292/jvms.65.1003

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Abstract

Parentage testing was performed in sixteen litters by canine artificial inseminations with frozen semen from different sires on Days 5 and 7 after the LH surge. It became apparent that only 25% of dams had superfecundation, but 43.8% of dams were whelped after insemination only on Day 5 after the LH surge and 31.3% of dams after insemination only on Day 7. Of the total 87 puppies, 46% were born after insemination on Day 5 after the LH surge and 54% after insemination on Day 7. This result strongly suggested that canine artificial insemination with frozen semen could be sufficiently successful also on Days 5 and 7 after the LH surge.

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