Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
Analysis of Protein Compositions and Surface Protein Epitopes of Anaplasma centrale and Anaplasma marginale
Yoshio NAKAMURAShin-ichiro KAWAZUTetsuro MINAMI
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1991 Volume 53 Issue 1 Pages 73-79

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Abstract

Protein composition was compared and epitopes were analyzed among the isolates of Anaplasma centrale and A. marginale by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting using bovine antisera and monoclonal antibodies, and enzyme-linked immunosorbent assay. Common and unique proteins were found among the isolates. All isolates tested had a major surface protein with an apparent molecular weight of 38 to 40 kilodalton which had slight molecular size variations between species. This protein was also a dominant immunogen to the host. At least two species-common epitopes, one of which might contain carbohydrate (s), were present on the major surface protein. One species-specific epitope was identified on the major surface protein of A. marginale isolates.

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© The Japanese Society of Veterinary Science
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