Modulation of systemic and mucosal immunity against an inactivated vaccine of          Newcastle disease virus by oral co-administration of live attenuated Salmonella            enterica serovar Typhimurium expressing chicken interleukin-18 and          interferon-α

Md. Masudur RAHMAN; Erdenebelig UYANGAA; Young Woo HAN; Jin HUR; Sang-Youel PARK; John Hwa LEE; Koanhoi KIM; Seong Kug EO

doi:10.1292/jvms.14-0495

Immunology

Modulation of systemic and mucosal immunity against an inactivated vaccine of Newcastle disease virus by oral co-administration of live attenuated Salmonella enterica serovar Typhimurium expressing chicken interleukin-18 and interferon-α

Md. Masudur RAHMAN, Erdenebelig UYANGAA, Young Woo HAN, Jin HUR, Sang-Youel PARK, John Hwa LEE, Koanhoi KIM, Seong Kug EO

Author information

Md. Masudur RAHMAN
College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Republic of Korea
Faculty of Veterinary and Animal Science, Sylhet Agricultural University, Sylhet 3100, Bangladesh
Erdenebelig UYANGAA
College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Republic of Korea
Young Woo HAN
College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Republic of Korea
Jin HUR
College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Republic of Korea
Sang-Youel PARK
College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Republic of Korea
Department of Bioactive Material Sciences, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea
John Hwa LEE
College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Republic of Korea
Department of Bioactive Material Sciences, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea
Koanhoi KIM
Department of Pharmacology, School of Medicine, Pusan National University, Yangsan 626-870, Republic of Korea
Seong Kug EO
College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Republic of Korea
Department of Bioactive Material Sciences, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea

Keywords: chicken interferon-α, chicken interleukin-18, mucosal immune response, Newcastle disease virus

JOURNAL FREE ACCESS

2015 Volume 77 Issue 4 Pages 395-403

DOI https://doi.org/10.1292/jvms.14-0495

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Abstract

Newcastle disease (ND) is a highly contagious disease of chickens causing significant economic losses worldwide. Due to limitations in the efficacy against currently circulating ND viruses, existing vaccination strategies require improvements, and incorporating immunomodulatory cytokines with existing vaccines might be a novel approach. Here, we investigated the systemic and mucosal immunomodulatory properties of oral co-administration of chicken interleukin-18 (chIL-18) and chicken interferon-α (chIFN-α) using attenuated Salmonella enterica serovar Typhimurium on an inactivated ND vaccine. Our results demonstrate that oral administration of S. enterica serovar Typhimurium expressing chIL-18 or chIFN-α provided enhanced systemic and mucosal immune responses, as determined by serum hemagglutination inhibition antibody and NDV Ag-specific IgG as well as NDV Ag-specific IgA in lung and duodenal lavages of chickens immunized with inactivated ND vaccine via the intramuscular or intranasal route. Notably, combined oral administration of S. enterica serovar Typhimurium expressing chIL-18 and chIFN-α significantly enhanced systemic and mucosal immunity in ND-vaccinated chickens, compared to single administration of S. enterica serovar Typhimurium expressing chIL-18 or chIFN-α. In addition, oral co-administration of S. enterica serovar Typhimurium expressing chIL-18 and chIFN-α provided enhanced NDV Ag-specific proliferation of peripheral blood mononuclear cells and Th1-biased cell-mediated immunity, compared to single administration of either construct. Therefore, our results provide valuable insight into the modulation of systemic and mucosal immunity by incorporation of immunomodulatory chIL-18 and chIFN-α using Salmonella vaccines into existing ND vaccines.

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