Identification of Group I-III Avian Adenovirus by PCR Coupled with Direct Sequencing of the Hexon Gene

Masaji MASE; Hiromichi MITAKE; Toshikazu INOUE; Tadao IMADA

doi:10.1292/jvms.71.1239

Abstract

Polymerase chain reaction (PCR) coupled with direct sequencing of the product of the hexon gene was applied to avian adenoviruses (formerly group I-III). The expected sizes of DNA fragments were successfully amplified by PCR from all of the group I-III avian adenoviruses with our designed primers. The resulting PCR product contained diagnostically relevant hexon sequences that could be used to identify the group and type of avian adenovirus.

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この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja

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