Correlations of Vibrio fischeri bacteria test data with bioassay data for other organisms.

Linear relationships of the median lethal concentrations of several hundreds of chemicals for a variety of organisms with Vibrio fischeri median effective concentrations are investigated. Significant correlations can be developed for many aquatic species including the fishes fathead minnow, bluegill, catfish, goldfish, goldorfe, guppy, killifish, rainbow trout, sheepshead minnow, and zebrafish; the water flea Daphnia sp.; such crustaceans as Artemia sp. and Crangon sp.; the ciliate Tetrahymena pyriformis; and algae, such as Chlorella sp. These interspecies relationships can be used to estimate order-of-magnitude type toxic effects of many substances for these aquatic organisms. Highly significant relationships can be obtained when selecting compounds on a chemical basis, such as alcohols, ketones, aromatics, etc., which allow the calculation of the compounds' toxicities to the corresponding aquatic species with increased accuracy and confidence. Analogous correlations with mammalian (rat and mouse) oral, intraperitoneal, and intravenous median lethal dose (LD50) data are much weaker than those for most aquatic species. However, there are significant differences between these three routes of administration and the intravenous LD50 data show the best relationship with the Vibrio data.


Introduction
The rising interest in finding alternatives to using large scale, expensive, and timeconsuming aquatic and terrestrial species tests for chemicals and commercial products has led to the investigation of several alternatives. One promising alternative is the use of bacteria for this purpose, especially Vibrio fischeri, formerly known as Photobacterium phosphoreum and commonly referred to as the Microtox test (Azur Environmental, Carlsbad, CA).
The photoluminescent bioassay uses a suspension of V. fischeri bacteria in saline water (to protect this marine bacterium from osmotic damage) and measures the reduction in light output of its natural luminescence on exposure to the toxicant of interest. In contrast to most aquatic bioassays, in which acute toxicities are usually measured over a period of 96 hr, the bacteria test can be performed in a matter of minutes. Furthermore, dormant bacteria concentrates can be kept frozen and ready for use for a prolonged period. Therefore, this test has generated widespread interest and has been adopted as a standardized test in several jurisdictions. It can be performed on demand in the laboratory without the need for keeping fish or mammals in larger scale facilities for extended periods. This convenience is obviously accompanied by savings in cost and time. Materials and specialized spectrophotometers are readily available from several commercial suppliers of the luminescence bioassay test systems, which are marketed under the names Microtox (Azur Environmental), Lumistox (Dr. Lange GmbH, Berlin, Germany), and Biotox (BioOrbit, Turku, Finland).
Considerable toxicity data for V. fischeri/P. phosphoreum are available in the form of monographs (1,2) covering over 1200 chemicals. More recently, interactive databases have become available on CD-ROM. These CD-ROMs are marketed under the names Computox (Environment Canada, Ottawa, Ontario), which covers 1500 substances (3), and TerraTox (Terra-Base Inc., Burlington, Ontario), which covers over 1700 substances (4), as well as specialized software for data analysis and toxicity estimation (4) available under the name TerraFit (TerraBase Inc.) Furthermore, symposium proceedings dealing with the use of photoluminescent bacteria for ecotoxicologic monitoring have been published (5). It is not surprising then that the V fischeri/P. phosphoreum data have become the largest published toxicity test data set for a single aquatic species. The biochemical mechanisms by which compounds exert a toxic or bioluminescence-reducing effect on the bacterium are only partly understood. In principle, the enzyme luciferase catalyzes the oxidation of reduced riboflavin phosphate, which is accompanied by emission of light. This process is linked with the microbial metabolism, and hence is directly linked to the toxic effect of a substance on the bacterium (6). Although different toxic mechanisms may exist in other organisms, substances highly toxic in one organism often also produce effects on quite different organisms. Isenberg, one of the original developers of the photoluminescent bacterial bioassay, describes this as testimony to the unity of life and shows that the median effective concentration (EC50) values for a variety of anesthetic gases are very similar for bacteria, mouse, dog, cat, and human (6).
Several studies have dealt with the relative sensitivity and comparability of the luminescent bacteria test with other, mostly aquatic, bioassays. Because this bioassay can be performed with relative ease and speed and at a limited cost, it is of particular interest as a test vehicle for routine monitoring once good correlations with local species have been established. A growing number of publications also use it for the ecotoxicologic assessment of contaminated soils and sediments and as a monitoring tool for site remediation. In research and product development studies, this test allows the rapid screening of novel compounds or a series of related compounds for their relative biologic activity.
Given the large number of available data and the simplicity and robustness of the test, it is of much interest to undertake qualitative and quantitative comparisons of the Microtox test data with those of other bioassays, particularly aquatic species. A variety of earlier studies on this subject include the works by Kaiser et al. (7), Zhao (8), Kaiser (9), Fort (10), Munkittrick (20), Qureshi (21), and Curtis (22). Given the multitude of species, bioassays, and chemical substances, this study emphasizes those species and bioassays where larger numbers of data are in common, perusing the largest available data compilation (4 Although not all of the above investigations allow quantitative comparisons, numerous data allow interspecies comparisons between V. fischeri and acute, subchronic, or chronic tests for a number of other aquatic species. In addition, recent research by Azur Environmental has resulted in the development of a chronic V fischeri bioassay as well as a mutagenicity assay using a dark mutant of the organism. The V. flscheri toxicity assay, commonly; known as the Microtox assay, is becoming a standardized toxicity assay in several countries (5), including Canada (23), France, Germany, Italy, Mexico, the Netherlands, Spain, Sweden, and the United States (24).

Data Sources, Notation, and Treatment
The data used here are those found in the handbook on Ecotoxicity of Chemicals to Photobacterium phosphoreum (2), augmented with new measurements (25), and other information and data as found in the Computox database (3) and the Terra-Tox/TerraFit software suite (4 (14) DeZwart and Slooff (19) DeZwart and Slooff (19) Ribo and Kaiser (20) Ribo and Kaiser (55) Xu et al. (13), Ribo and Kaiser (55) DeZwart and Slooff (19) given to 30-min EC50 data over 15-min data, and 15-min EC50 over 5-min data, where available. All available data were used for the development of the correlations presented here, i.e., no preselection of any kind was made.
For the data analysis and graphing, the TerraTox/TerraFit software suite (4) was used. This program allows automated trimming of data pairs exceeding defined specifications. In this work, data removed in the trimming process are those for which the measured value differed by more than 2G from the regression-derived estimated value for the end point complementary to the bacteria value. This corresponds roughly to a 95% confidence interval. The number of compounds so removed are given in the figure captions.
The data quality for all of the different end points in this paper varies widely and is uncertain in several instances. This variation stems both from the specifics of each test system used, for example, static systems with nominal concentrations and no consideration of abiotic degradation or loss through volatilization, and lack of detailed information on certain experimental conditions, such as pH, purity of chemicals tested, and related aspects. Therefore, in many instances confidence in the data themselves varies; for some of the data, the accuracy may vary by 0.5 orders of magnitude or more from values that would be found with more elaborate test systems and conditions for the same end point. Some comments on data quality and erroneous data are provided by Kaiser (26).

Correlations with Fathead Minnow Acute Toxicity Data
One of the largest single species aquatic test data sets, the 96-hr acute lethal concentration bioassay data for fathead minnow (Pimephales promelas), has data for approximately 800 chemicals, most of which have been measured by the Center for Lake Superior Environmental Studies, University of Wisconsin-Superior (Superior, WI) (27)(28)(29)(30)(31). At present there are published Microtox data for approximately 1700 compounds; the majority of these values originated at the National Water Research Institute, Burlington, Ontario. Between these two data sets there are now in excess of 400 individual compounds in common and their respective toxicities span 10 orders of magnitude on a molar basis. Figure 1 gives a plot of these fathead minnow and Microtox toxicity data after trimming compounds of > 2ca, which reduces the number of data pairs from 439 to 420. Table 2 gives the statistical details  for this and other correlations and Table 3 shows a list of the compounds removed by trimming with their respective values. They include silver nitrate (which is difficult to determine in the Microtox test because of the normal presence of chloride ion for osmotic protection for the bacteria); four chemicals with terminal = CRR' groups, where R or R'= H, Cl, which are recognized gill membrane irritants (vinylcarbazole, resmethrin, telone II, and allyl alcohol); three amines for which the pH has a critical influence on the apparent toxicity (ethylaniline, ethanolamine, and triethanolamine); several highly specific pesticides (DDT, dieldrin, endosulfan, aldicarb, fenvalerate, terbufos); and four other compounds for which no explanation appears readily available (two esters, one acid, and one phenol derivative). The observed fish toxicity for the amines and oleic acid was significantly lower than predicted from the equation in Table 2; for the other compounds in Table 3, observed fish toxicity was significantly higher. Figure 1 shows a strong correlation between the fathead minnow median lethal concentration (LC50) and bacteria EC50 values over a toxicity range of over 10 orders of magnitude in molar activity. The entire set is collated strictly on the basis of compounds measured in both tests without any other selection process. Although there 0 E cX is a considerable standard deviation of 0.83 pT units, this correlation is sufficiently strong to use it for a quick order-of-magnitude estimation of fish toxicities from those found in the Microtox test. This correlation can be applied to many substances because it also covers a large variety of chemical classes, including alcohols and phenols, aliphatic, aromatic, and heterocyclic compounds, acids, ketones, nitriles, nitro-, fluoro-, chloro-, and bromo-substituted chemicals, and various pesticides, including fungicides, herbicides, insecticides, and others. Where more reliable predictions are desired, selection of the chemicals by class, mode of action (if known), or similar functions will be required to increase the quality of the regression and reduce the standard deviation of the estimate. Such an approach can produce significant improvements and has been successfully demonstrated for many series of homologs for these and other species; some examples are given below.
Using this information and the now well established relationship of Microtox and fathead minnow data, we can make predictions for the latter for compounds that have not been directly measured in that system. For example, the TerraFit prediction system (4) allows the easy computation of approximately 1200 fathead minnow toxicity (pT) data. Of these, values in excess of 4.00 (i.e., highly toxic substances) can be calculated for busan, chloroxuron, several tributyltin derivatives,    and zebrafish (Brachydanio rerio), 96-hr LC50, together with some correlations on smaller data sets from the literature. The new correlations are for data sets ranging from 44 (red killifish) to 439 (fathead minnow) data pairs and cover a toxicity range of 5 (zebrafish) to 10 (several species) orders of magnitude on a molar basis. Table 2 also gives the correlation of V fischeri EC50 values with fish composite values. This composite is made up of the arithmetic mean pT value of all individual fish LC50 data (pT values) where available in the database, using a limit of 1 pT value per end point (32) and all available Microtox pT values. This correlation covers 647 individual chemicals and 10 orders of magnitude each for the fish and Microtox values. The highly significant correlation (p< 0.00 1) obtained after one trimming, covering 616 compounds, is shown in Figure 3. The slope for the composite fish regression (b= 0.81, Table 2) is identical to that for the fathead minnow regression (trimmed once) and approximately in the middle of the range of the slopes of all individual fish species regressions, an indication of the generality of these relationships. Furthermore, as the different fish species analyzed include cold water (e.g., rainbow trout), warm water (e.g., fathead minnow), and tropical fish (zebrafish), as well as marine-(sheepshead minnow) and freshwater-inhabiting species (all others), the results indicate a wide range of applicability of these Vibrio to fish correlations for the order-of-magnitude estimation of the acute toxicity of chemicals. As mentioned for the fathead minnow, for more accurate estimations and reduced standard deviations, selection of smaller sets of relevant chemicals by mode of action, chemical class, or other proximity indicators should precede the analysis and prediction. In this field, attempts are presently underway to develop and apply more powerful algorithms, including multiple linear correlations and neural networks to improve the predictive capabilities of these correlations (33,34).
Correlations with Toxicity Data for Other Aquatic Organisms A detailed list of correlations of V fischeri EC50 values with acute toxic values to numerous organisms is given in Table 1. Besides the relationships for several fish species, explored in some detail above, a considerable volume of data allows the analysis of interspecies correlations between this bacterium and other aquatic organisms. Table 4 gives several regressions developed for an alga  Table 4 with those for the fish (Table 2) shows generally similar slopes and standard deviations of the regressions. The somewhat higher standard deviations (1.15 to 1.65) for the crayfish and Crangon sp. can be explained by a preponderance of highly toxic substances (pesticides) in these data sets. The rather low slopes of 0.57 to 0.69 observed for the cor-.7 relations with the Tetrahymena data are 5 thought to arise from the high level of organic matter present in the culture medium for this organism, which leads to adsorption and complexation of the toxicants (35). The largest data set in this group, found for D. magna, covers 9 orders of magnitude in toxicity on a molar basis and leads to a highly significant correlation with a standard error of 0.85 pT units. ,st versus Given the similarity of these equations with d, 9. those for the fish, remarks concerning the applicability and accuracy of the predictive use of the regressions for fish apply to the algae and crustacean correlations as well. 0 These data are entirely nonselected and obviously cover compounds of very different nature, from nonpolar narcotics to o highly specific mode of action pesticides. Therefore they provide estimates for the average activity of chemicals, and if more accurate predictions are desired, more narrowly defined subsets are required.

Correlations for Specific
Groups of Substances 6.0 The highly promising results on interspecies toxicity correlations, as outlined above, raise the question as to their possible refinement by narrowing the applications to specific chemical classes or substances of the same mode of action or any other group that can be defined in a biochemical or chemical sense. Indeed, a plot of Microtox versus fathead minnow rersus the data for 18 halogen-free alcohol pairs , 12. shows an excellent relationship over   Table 5; number of data trimmed, 2.  Table 5; number of data trimmed, nil. approximately 6 orders of magnitude; the statistics are given in Table 5. Similar correlations can be developed for other groups for example compounds with the fragment "ol" in the name, i.e., mostly alcohols and phenols; compounds with the fragment "enol" in the name, primarily phenols; or compounds with the fragment "non" in the name, primarily ketones. Other examples of a simple selection of chemicals is the search for substances with the fragment "ene" in the name, mostly polynuclear aromatic hydrocarbons, the plot for which is shown in Figure 4. Another type of selection, based on the number of carbon atoms in the molecule, is shown in Figure 5 for compounds that have exactly six carbon atoms in the chemical formula (C6). The regression statistics for the above selections are given in Table 5 and it is apparent from both these graphs and regression models that highly significant correlations exist between the V. fischeri assay and the fathead minnow data for these groups of chemicals. Therefore, such selections can significantly improve the reliability of predicted values. Of course, similar analyses can be undertaken for each of the many species and end points for which the data are available to develop such models. Because there are also significant correlations between the Vibrio data and the bioassay results for other aquatic species, as shown in Tables 2 and 4, it is expected that these predictive models can be expanded to include several other species and toxicity end points.

Correlations with Toxicity Data for Terrestrial Species
There is a limited number of studies on the interspecies correlations of Vibrio bacteria toxicity data with those of terrestrial species, mainly rat and mouse, as well as some plants, earthworms, frogs, yeasts, and other bacteria (Table 1). In the latter, the data sets studied are too limited to draw any general conclusions. In contrast, for the mammalian species there is a large number (> 500 compounds) of data that have been investigated for the routes of exposure, i.e., oral, intraperitoneal, and intravenous administration (7,10). The results show some general correlations, but the regression slopes are generally low and the standard errors of the estimates too high to be of much practical use, i.e., predictive capability, at this time. There is, however, a marked difference in the slopes of the oral, intraperitoneal, and intravenous routes of administration, with the oral route having the lowest slope and the intravenous route having the highest. This observation reflects the relatively higher degree of metabolization and detoxification or kinetically controlled limited uptake (especially for compounds with high lipophilicity and/or large molecular size) versus the intravenous route of exposure, which bypasses the intestine-blood barrier (10). For 171 chemicals, the correlation coefficient between mammalian (rat and mouse) intravenous median lethal dose (LD50) and V. fischeri EC50 values was r= 0.75 with a standard error of 0.52 (10). Although this standard error is even smaller than for most of the correlations with the aquatic species shown above, the correlation's usefulness is limited because of the much smaller range of mammalian toxicity values in it. This problem is common to all attempts at correlating these mammalian LD50 data with physicochemical or other toxic response data; it is basically caused by the incongruent nature of these data rather than by the organism itself. Where compatible data are used, mammalian and bacteria data show quite close similarities in their response to the chemical challenge (6).

Practical Applications
There are numerous publications on compound-specific qualitative and quantitative comparisons of Vibrio bacteria test data with those for other organisms. Table  1 lists some of the major studies that deal with individual organic, inorganic, and organometallic compounds. In addition, there are many other publications on similar correlations of the toxic effects of environmental extracts, such as leachates, sediment elutriates, surface and groundwater samples, complex effluents from industrial and municipal origins, treated and untreated waste materials, and others (36). Most authors of these reports recommend use of the bacterial test system as a component in a battery of tests to assess ecotoxicologic effects of complex effluents, contaminated sediments, and so forth. Where only a relative assessment is required, such as the measurement of spatial or temporal variation in a defined area or system, the use of the bacteria assay can be sufficient to delineate the gradient. In fact, some waste treatment authorities in Great Britain measure continuously the toxicity of incoming waste streams prior to treatment to prevent upset and maintain the biologic treatment system (37). In situations of sudden increase in toxicity (usually not identifiable from other measurements, such as biochemical oxygen demand), separation and special treatment are undertaken.
In terms of predictive applications to new chemicals or to those for which little else is known, extrapolation of the bacteria test to fish and other organisms can be done with varying degrees of confidence. The level of confidence for a new substance is a function of both the chemical complexity of the molecule in question and the availability of data for related compounds. For example, the effects of compounds with fewer and/or noninteracting functional groups can be predicted more readily and with a higher confidence than those for which electronic and steric conditions or unknown conformational changes may influence the properties and effects and potential specific interaction with receptor sites.

Conclusions
There are many highly significant interspecies relationships between Vibrio and numerous other aquatic species, which can be exploited to make confident predictions of one end point from another. This works particularly well for compounds of relatively simple chemical structure with one reactive or functional group, such as the hydroxyl or keto moieties. As is the case with many other bioassays, chemicals of more complex structure, such as those with several functional groups interacting, with tautomeric and conformational changes, or with ionization occurring near physiologic pH values, are frequently associated with highly specific effects on a particular organism or biochemical function, and hence are more difficult to model. Although the available evidence suggests that there is a well known mean of effects for all compounds of similar polarity, molecular weight, size, functionality, etc., the potential deviation from this mean appears to increase with this complexity. As a result, less reliable predictions can be made for such substances, irrespective of whether basic physicochemical structure-activity relationships are used, such as correlations with the octanol/water partition coefficient or from interspecies correlations. There are also some noteworthy studies on Vibrio bacteria-mammalian toxicity relationships that indicate limited usefulness because of the nature of the mammalian data. However, the quality of these relationships increases markedly from oral LD50 to intraperitoneal LD50 to intravenous LD50 data. Therefore, future research efforts should be directed toward the latter type of mammalian data. Also, research into the development of multiple linear regression and neural net-type-based relationships is under way. In terms of speed, simplicity, and cost effectiveness, the Microtox test provides an excellent means of quickly measuring the acute toxic effect of either individual substances or complex mixtures on the Vibrio bacterium and, by way of proven inference, on a wide variety of higher organisms, particularly in the aquatic sphere. This test has become a standardized system for ecotoxicologic assessment in a variety of jurisdictions and is successfully being used to assess and improve practical treatment of effluents, soils, sediments, leachates, and other contamination problems.