1991 Volume 55 Issue 6 Pages 1547-1552
We have cloned a DNA fragment bearing the putative FLO1 gene that controls flocculence in Saccharomyces cerevisiae and showed that the cloned gene is on chromosome I. The flocculation property conferred by the cloned gene in a YCp-type plasmid (single-copy plasmid) was diminished in MATa/MATα. cells, which suggests that the expression of the putative FLO1 gene may be repressed by the MAT information. Introduction of the DNA fragment in a YRp-type plasmid (multi-copy plasmid) into several non-flocculent industrial yeast strains, including bottom- and top-fermenting brewing yeasts, and those for making whisky, wine, sake, and shochu could convert them to flocculent ones.
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