An alkalophilic actinomycete, strain OPC-15, which was isolated from a soil sample, produced phenazine antibiotics in the mycelium. From the results of taxonomic studies, this strain was designated as Nocardiopsis dassonvillei OPC-15. N. dassonvillei OPC-15 produced different phenazine antibiotics under different culture conditions (incubation period, incubation temperature and temperature shift). 1, 6-Dihydroxyphenazine (III) was obtained from the mycelium on incubation for 6-8 days at 27°C, while 1, 6-dihydroxyphenazine 5, 10-dioxide (I, iodinin) was isolated on incubation for 6 days at 27°C followed by further incubation for 2 days at 4°C.
1, 6-Dihydroxyphenazine 5-monooxide (II) was purified from the mycelium cultured under both these two sets of cultural conditions. These results suggest that the N-oxidation of III was catalyzed enzymatically with the temperature shift from 27°C to 4°C.

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