Effect of heating, freeze-drying, frozen storage and coagulation by chymosin on the exchangeability of colloidal calcium with soluble calcium in milk was investigated by the radioisotopic method previously reported. Heating at 100°C for 30min caused a slight decrease of the exchangeability. Frozen storage at -20°C for 120 days resulted in a marked decrease of the exchangeability accompanying a decrease of soluble calcium. Coagulation by chymosin gave no significant effect on the amount of soluble calcium or on the exchangeability of colloidal calcium.

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