Diapause Hormone B: Its Selective Extraction and Isolation from the Silkworm, Bombyx mori

Minoru ISOBE; Kinsaku HASEGAWA; Ichiro KUBOTA; Toshio GOTO

doi:10.1271/bbb1961.40.1189

Agricultural and Biological Chemistry

Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369

Diapause Hormone B: Its Selective Extraction and Isolation from the Silkworm, Bombyx mori

Minoru ISOBE, Kinsaku HASEGAWA, Ichiro KUBOTA, Toshio GOTO

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JOURNAL FREE ACCESS

1976 Volume 40 Issue 6 Pages 1189-1199

DOI https://doi.org/10.1271/bbb1961.40.1189

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Abstract

A selective extraction procedure of the diapause hormone-B (DH-B) from male silkworm adult heads is described. By this new method a highly active extract (1 DH unit in 30_??_60μg) can be obtained easily without chromatographic purification. It was further purified by successive gel permeation chromatographies to give finally pure DH-B having an activity of 1 DH unit in 2 μg.

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